Cloned (Comment) | Organism |
---|---|
gene dhs, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3), coexpression with His-tagged DOHH (deoxyhypusine hydroxylase) in Escherichia coli strain BL21-CodonPlus (DE3), quantitative real-time PCR expression analysis | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, recombinant His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FI94 | gene dhs | - |
Arabidopsis thaliana Col-0 | Q9FI94 | gene dhs | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and ultrafiltration, followed by cleavage of the His-tag through protease 3C and elimination via affinity chromatography | Arabidopsis thaliana |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, recombinant His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
deoxyhypusinesynthase | - |
Arabidopsis thaliana |
DHS | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | the plant stress hormone abscisic acid causes a reduction in the hypusination of eIF5A1 probably through the post-transcriptional alteration of DHS activity | Arabidopsis thaliana |