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Information on EC 2.5.1.46 - deoxyhypusine synthase
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2.5.1.46 deoxyhypusine synthaseIUBMB Comments
The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor. The reaction occurs in four steps: NAD+-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (Lys329 for the human enzyme; 1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d). Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor. Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29, deoxyhypusine monooxygenase.
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Word Map
- 2.5.1.46
- polyamine
- gc7
- homospermidine
-
pyrrolizidine
- n1-guanyl-1,7-diaminoheptane
-
nepsilon-4-amino-2-hydroxybutyllysine
-
aminobutylation
-
hypusine-containing
- 1,7-diaminoheptane
-
deoxyhypusine-containing
- butylamine
-
polyamine-derived
-
eif-4d
- medicine
- 1,3-diaminopropane
- triamine
-
wolff
- drug development
- analysis
The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota
Reaction Schemes
Synonyms
deoxyhypusine synthase, dhs34, cpdhs, pf3d7_1412600, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
deoxyhypusine synthase
deoxyhypusine synthase (Caulobacter crescentus gene CC0359)
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deoxyhypusine synthase (Halobacterium strain NRC-1 gene dhs)
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-
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-
deoxyhypusine synthase (human clone 30649 gene DHPS subunit reduced)
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-
deoxyhypusine synthase (Nicotiana tabacum gene DHS1)
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-
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deoxyhypusine synthase (Senecio vernalis gene DHS1)
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-
deoxyhypusinesynthase
DHPS
DHS
DHS34
DHSc
DHSp
Dys1p
EC 1.1.1.249
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-
formerly
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HVO 2297
synthase, deoxyhypusine
-
-
-
-
TbDHSc
TbDHSp
DHS
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dehydrospermidine + [enzyme]-lysine = N-(4-aminobutylidene)-[enzyme]-lysine + propane-1,3-diamine
(1b)
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-
-
N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + NADH + H+ = [eIF5A-precursor]-deoxyhypusine + NAD+
(1d)
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-
-
N-(4-aminobutylidene)-[enzyme]-lysine + [eIF5A-precursor]-lysine = N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + [enzyme]-lysine
(1c)
-
-
-
spermidine + NAD+ = dehydrospermidine + NADH
(1a)
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-
-
[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
first reaction
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[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
the eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor, the second reaction being catalysed by EC 1.14.99.29, deoxyhypusine monooxygenase. The reaction of this enzyme occurs in four steps: i. NAD-dependent dehydrogenation of spermidine, ii. formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue, Lys329 for the human enzyme, iii. transfer of the same 4-aminobutylidene group from the enzyme intermediate to the eIF5A precursor, iv. reduction of the eIF5A-imine intermediate to form a deoxyhypusine residue. Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminobutyl group transfer
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-
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-
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SYSTEMATIC NAME
IUBMB Comments
[eIF5A-precursor]-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming)
The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor. The reaction occurs in four steps: NAD+-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (Lys329 for the human enzyme; 1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d). Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor. Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29, deoxyhypusine monooxygenase.
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CAS REGISTRY NUMBER
COMMENTARY
127069-31-2
deoxyhypusine synthase
171041-87-5
human clone 30649 gene DHPS subunit reduced
253180-41-5
Nicotiana tabacum gene DHS1
259168-76-8
Senecio vernalis gene DHS1
302492-11-1
Halobacterium strain NRC-1 gene dhs
332963-67-4
Caulobacter crescentus gene CC0359
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-ethylspermidine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-deoxyhypusine + ?
-
-
-
?
1-methylspermidine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-deoxyhypusine + ?
-
-
-
?
8-ethylspermidine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-ethyldeoxyhypusine + ?
-
-
-
?
8-methylspermidine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-methyldeoxyhypusine + ?
-
-
-
?
aminopropylcadaverine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-homodeoxyhypusine + ?
-
-
-
?
homospermidine + [eIF5A-precursor]-lysine
[eIF5A-precursor]-deoxyhypusine + ?
-
-
-
?
N-(3-aminopropyl)-1,4-diamino-cis-but-2-ene + putrescine
(1Z)-N4-(4-aminobutyl)but-1-ene-1,4-diamine + 1,3-diaminopropane
-
-
-
?
N-(3-aminopropyl)-1,4-diamino-cis-but-2-ene + [eIF5A-precursor]-lysine
?
-
-
-
?
N-(3-aminopropyl)-1,4-diamino-trans-but-2-ene + putrescine
(1E)-N4-(4-aminobutyl)but-1-ene-1,4-diamine + 1,3-diaminopropane
-
-
-
?
N-(3-aminopropyl)-1,4-diamino-trans-but-2-ene + [eIF5A-precursor]-lysine
?
-
-
-
?
[eIF5A-1-precursor]-lysine + spermidine
[eIF5A-1-precursor]-deoxyhypusine + propane-1,3-diamine
[eIF5A-2-precursor]-lysine + spermidine
[eIF5A-2-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
[eIF5A-precursor]-lysine + homospermidine
[eIF5A-precursor]-deoxyhypusine + putrescine
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
[eIF5A-precursor]-lysine42 + spermidine
[eIF5A-precursor]-lysine42-deoxyhypusine + propane-1,3-diamine
[TveIF-5A-precursor]-lysine + spermidine
[TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
spermidine
1,3-diaminopropane + DELTA1-pyrroline
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reaction in absence of eIF5A-precursor
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?
spermidine
1,3-diaminopropane + DELTA1-pyrroline
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reaction in absence of eIF5A-precursor
-
?
spermidine
1,3-diaminopropane + DELTA1-pyrroline
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reaction in absence of eIF5A-precursor
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?
spermidine
1,3-diaminopropane + DELTA1-pyrroline
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reaction in absence of eIF5A-precursor
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?
spermidine + NAD+
dehydrospermidine + NADH
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-
-
?
[eIF5A-1-precursor]-lysine + spermidine
[eIF5A-1-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-1-precursor]-lysine + spermidine
[eIF5A-1-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor] + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor] + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor] + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor] + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-L-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + (R)-3-methylspermidine
?
enzyme DHS prefers the (R)- over the (S)-enantiomer as a substrate
-
-
?
[eIF5A-precursor]-lysine + (R)-3-methylspermidine
?
no activity with the (S)-enantiomer
-
-
?
[eIF5A-precursor]-lysine + 2-methylspermidine
?
-
the S-enantiomer of 2-MeSpd, which supports long-term growth, is a good substrate for DHS in vitro, whereas the R isomer is not
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
transfer of a 4-aminobutyl moiety group
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
transfer of a 4-aminobutyl moiety group
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
deoxyhypusine synthase is the first of the two enzymes that catalyze the maturation of eukaryotic initiation factor 5A. Synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
bovine enzyme is approximately 26times as effective as the human enzyme in catalyzing the reaction
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
the enzyme catalyzes the first reaction in a two-step conversion of inactive eIF-5A to its active form
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
hypusination of Lys56
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
the enzyme catalyzes the first reaction in a two-step conversion of inactive eIF-5A to its active form
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
ir
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
reversal of reaction in presence of NAD+
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
r
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
first step in the biosynthesis of hypusine
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
first step in the biosynthesis of hypusine
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
posttranslational activation of eIF-5A which has a specialized role in proliferation and in serum starvation-induced apoptosis in endothelial cells
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
overall reaction
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
bovine enzyme is approximately 26times as effective as the human enzyme in catalyzing the reaction
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
DHS catalyzes a NAD-dependent cleavage between N4 and C5 of spermidine and the transfer of the butylamine moiety to the ?-amino group of a specific lysine residue of the eIF5A precursor, eIF5A(Lys), to form the deoxyhypusine residue. The enzyme is modification is strictly specific for one single lysine residue of the cellular protein eukaryotic initiation factor 5A (eIF5A) and does not work on short peptides
1,3-diaminopropane is released as a byproduct from spermidine cleavage
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
formation of [N(epsilon)-(4-amino-2-hydroxybutyl)lysine]
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
overall reaction
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
the enzyme is involved in synthesis of synthesis of homospermidine
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
the enzyme catalyzes the first reaction in a two-step conversion of inactive eIF-5A to its active form
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
posttranslational activation of the initiation factor 5A
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
-
-
-
?
[eIF5A-precursor]-lysine + spermidine
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
-
?
[eIF5A-precursor]-lysine42 + spermidine
[eIF5A-precursor]-lysine42-deoxyhypusine + propane-1,3-diamine
-
substrate [eIF5A-precursor]-lysine42 of Haloferax volcanii
overall reaction
-
?
[eIF5A-precursor]-lysine42 + spermidine
[eIF5A-precursor]-lysine42-deoxyhypusine + propane-1,3-diamine
-
substrate [eIF5A-precursor]-lysine42 of Haloferax volcanii
overall reaction
-
?
[TveIF-5A-precursor]-lysine + spermidine
[TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[TveIF-5A-precursor]-lysine + spermidine
[TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue
-
-
?
[TveIF-5A-precursor]-lysine + spermidine
[TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
-
-
?
[TveIF-5A-precursor]-lysine + spermidine
[TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue
-
-
?
additional information
?
-
-
deoxyhypusine synthase-suppressed Brassica napus plants have larger leaves as well as enhanced seed yield, this appears to reflect increased tolerance to chronic sublethal stress
-
-
?
additional information
?
-
-
recombinant deoxyhypusine synthase from Haloferax volcanii does not show any activity under varying aIF5A concentration and substrate concentration unsing spermidine, agmatine, cadaverine, and putrescine. In vivo, the eIF5A-precursor in Haloferax volcanii is shown to be deoxyhypusinylated
-
-
?
additional information
?
-
-
recombinant deoxyhypusine synthase from Haloferax volcanii does not show any activity under varying aIF5A concentration and substrate concentration unsing spermidine, agmatine, cadaverine, and putrescine. In vivo, the eIF5A-precursor in Haloferax volcanii is shown to be deoxyhypusinylated
-
-
?
additional information
?
-
-
as racemic mixtures, only 2-MeSpd and 3-MeSpd support long-term growth (9-18 days) of spermidine-depleted DU-145 cells, whereas gamma-MeSpd and omega-MeSpd do not
-
-
?
additional information
?
-
-
development of a radioactive assay method for detection of spermidine incorporation into eIF5A, overview
-
-
?
additional information
?
-
(R)-3-methylspermidine competes with spermidine for cellular uptake less efficiently than the (S)-3-methylspermidine
-
-
?
additional information
?
-
(R)-3-methylspermidine competes with spermidine for cellular uptake less efficiently than the (S)-3-methylspermidine
-
-
?
additional information
?
-
-
the DHSL20 protein lacks a critical lysine residue, and the recombinant protein shows no DHS activity in vitro, while DHS34 contains the critical lysine residue, and the recombinant DHS34 effectively catalyzes deoxyhypusine synthesis
-
-
?
additional information
?
-
the DHSL20 protein lacks a critical lysine residue, and the recombinant protein shows no DHS activity in vitro, while DHS34 contains the critical lysine residue, and the recombinant DHS34 effectively catalyzes deoxyhypusine synthesis
-
-
?
additional information
?
-
the DHSL20 protein lacks a critical lysine residue, and the recombinant protein shows no DHS activity in vitro, while DHS34 contains the critical lysine residue, and the recombinant DHS34 effectively catalyzes deoxyhypusine synthesis
-
-
?
additional information
?
-
no synthesis of [eIF5A-precursor]-hypusine
-
-
?
additional information
?
-
-
no synthesis of [eIF5A-precursor]-hypusine
-
-
?
additional information
?
-
no synthesis of [eIF5A-precursor]-hypusine
-
-
?
additional information
?
-
-
mutants of substrate [eIF5A-1-precursor]-lysine with changes in residue K50, and mutants G52A and K55A are no substrates. The hypusine site loop has critical importance in eIF5A function and hypusine modification
-
-
?
additional information
?
-
the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29
-
-
?
additional information
?
-
-
the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29
-
-
?
additional information
?
-
formation of a TvDHS-TveIF-5A complex
-
-
?
additional information
?
-