BRENDA - Enzyme Database show
show all sequences of 2.5.1.44

Purification and characterization of homospermidine synthase in Acinetobacter tartarogenes ATCC 31105

Yamamoto, S.; Nagata, S.; Kusaba, K.; J. Biochem. 114, 45-49 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
preincubation, 1 mM, 30 min prior to assay causes 50% inhibition
Acinetobacter tartarogenes
N-ethylmaleimide
preincubation, 1 mM, 30 min prior to assay causes 91% inhibition
Acinetobacter tartarogenes
NADH
Ki: 0.006 mM
Acinetobacter tartarogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
putrescine
-
Acinetobacter tartarogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
required for full activity, optimal activity at 50 mM
Acinetobacter tartarogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Acinetobacter tartarogenes
102000
-
gel filtration
Acinetobacter tartarogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Acinetobacter tartarogenes
-
sym-homospermidine + NH3
-
Acinetobacter tartarogenes
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter tartarogenes
-
ATCC 31105
-
Purification (Commentary)
Commentary
Organism
-
Acinetobacter tartarogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.41
-
-
Acinetobacter tartarogenes
Storage Stability
Storage Stability
Organism
1°C, 20 mM, Tris/HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM putrescine, 0.02% NaN3
Acinetobacter tartarogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
-
288700
Acinetobacter tartarogenes
sym-homospermidine + NH3
-
288700
Acinetobacter tartarogenes
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Acinetobacter tartarogenes
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
-
Acinetobacter tartarogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Acinetobacter tartarogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Acinetobacter tartarogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
preincubation, 1 mM, 30 min prior to assay causes 50% inhibition
Acinetobacter tartarogenes
N-ethylmaleimide
preincubation, 1 mM, 30 min prior to assay causes 91% inhibition
Acinetobacter tartarogenes
NADH
Ki: 0.006 mM
Acinetobacter tartarogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
putrescine
-
Acinetobacter tartarogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
required for full activity, optimal activity at 50 mM
Acinetobacter tartarogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
2 * 52000, SDS-PAGE
Acinetobacter tartarogenes
102000
-
gel filtration
Acinetobacter tartarogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 putrescine
Acinetobacter tartarogenes
-
sym-homospermidine + NH3
-
Acinetobacter tartarogenes
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acinetobacter tartarogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.41
-
-
Acinetobacter tartarogenes
Storage Stability (protein specific)
Storage Stability
Organism
1°C, 20 mM, Tris/HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM putrescine, 0.02% NaN3
Acinetobacter tartarogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 putrescine
-
288700
Acinetobacter tartarogenes
sym-homospermidine + NH3
-
288700
Acinetobacter tartarogenes
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 52000, SDS-PAGE
Acinetobacter tartarogenes
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
-
Acinetobacter tartarogenes
Other publictions for EC 2.5.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739378
Lopez-Gomez
-
Homospermidine synthase contri ...
Rhizobium tropici, Rhizobium tropici Rt CIAT899
Plant Soil
404
413-425
2016
-
-
1
-
1
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739672
Krossa
Comprehensive structural chara ...
Blastochloris viridis
Sci. Rep.
6
19501
2016
-
-
-
1
2
-
-
-
-
1
-
2
-
5
-
-
-
2
-
-
-
-
6
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
3
3
-
-
-
739108
Li
Different polyamine pathways f ...
Paramecium tetraurelia, Paramecium tetraurelia d4-2
Mol. Microbiol.
97
791-807
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
704662
Shaw
Evolution and multifarious hor ...
Acinetobacter tartarogenes, Blastochloris viridis, Bradyrhizobium japonicum, Opitutus terrae, Paramecium tetraurelia, Ralstonia phage phiRSL1, Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
-
-
7
-
-
-
-
3
-
-
-
-
-
15
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
7
-
-
-
-
-
7
7
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
7
-
6
-
-
3
6
-
-
-
-
1
1
-
3
3
288701
Tholl
Purification, molecular clonin ...
Blastochloris viridis
Eur. J. Biochem.
240
373-379
1996
-
-
1
-
-
-
-
1
-
-
3
1
-
6
-
-
1
-
-
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
3
1
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288702
Ober
-
Homospermidine synthase of Rho ...
Blastochloris viridis
J. Gen. Appl. Microbiol.
42
411-420
1996
-
-
-
-
-
-
-
4
-
1
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
1
-
1
-
-
-
-
-
1
-
-
9
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288699
Böttcher
-
Biosynthesis of pyrrolizidine ...
Blastochloris viridis
Can. J. Chem.
72
80-85
1994
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
1
1
1
9
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
1
-
-
-
1
-
1
1
1
9
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288700
Yamamoto
Purification and characterizat ...
Acinetobacter tartarogenes
J. Biochem.
114
45-49
1993
-
-
-
-
-
-
3
1
-
1
2
1
-
6
-
-
1
-
-
-
1
1
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
-
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288698
Srivenugopal
Enzymic synthesis of sym-homos ...
Lathyrus sativus, Santalum album
Biochem. J.
190
461-464
1980
-
-
-
-
-
-
4
1
-
-
1
2
-
2
-
-
1
-
-
2
2
-
2
-
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
1
2
-
-
-
1
-
2
2
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
288697
Tait
The formation of homospermidin ...
Blastochloris viridis, Blastochloris viridis N.C.I.B. 10028
Biochem. Soc. Trans.
7
199-200
1979
-
-
-
-
-
-
4
2
-
3
1
2
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
3
1
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-