Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as His-tagged protein | Eucommia ulmoides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isopentenyl diphosphate | - |
Eucommia ulmoides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
dimethylallyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.0097 | - |
geranyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.011 | - |
geranyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.0146 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.0202 | - |
isopentenyl diphosphate | fixed substrate: farnesyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.028 | - |
isopentenyl diphosphate | fixed substrate: farnesyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.0298 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.0302 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.0309 | - |
dimethylallyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.0483 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.187 | - |
(2E,6E)-farnesyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.275 | - |
(2E,6E)-farnesyl diphosphate | fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides | |
Co2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides | |
Mg2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides | |
Mn2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides | |
Ni2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides | |
Zn2+ | the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) | Eucommia ulmoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Eucommia ulmoides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | Eucommia ulmoides | - |
diphosphate + geranylgeranyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | Eucommia ulmoides | - |
diphosphate + geranyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | Eucommia ulmoides | - |
diphosphate + (2E,6E)-farnesyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eucommia ulmoides | Q94IE8 | - |
- |
Eucommia ulmoides | Q94IE9 | - |
- |
Purification (Comment) | Organism |
---|---|
purified by Co2+ affinity chromatography | Eucommia ulmoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Eucommia ulmoides | diphosphate + geranylgeranyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate | Eucommia ulmoides | diphosphate + geranylgeranyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | - |
Eucommia ulmoides | diphosphate + geranyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate | Eucommia ulmoides | diphosphate + geranyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Eucommia ulmoides | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate | Eucommia ulmoides | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EuFPS1 | - |
Eucommia ulmoides |
EuFPS2 | - |
Eucommia ulmoides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | enzyme EuFPS1 | Eucommia ulmoides |
20 | 30 | enzyme EuFPS2 | Eucommia ulmoides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
1 h, stable. Activity dramatically decreases at higher temperatures | Eucommia ulmoides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | enzyme EuFPS1 | Eucommia ulmoides |
7 | 9.5 | enzyme EuFPS2 | Eucommia ulmoides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.102 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: dimethylallyl diphosphate, fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.1052 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, substrate: isopentenyl diphosphate, fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.1187 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: dimethylallyl diphosphate, fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.1264 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.2249 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: geranyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides | |
0.2373 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: farnesyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.2473 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: geranyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C | Eucommia ulmoides | |
0.2673 | - |
isopentenyl diphosphate | fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: farnesyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C | Eucommia ulmoides |
General Information | Comment | Organism |
---|---|---|
physiological function | essential enzyme in the biosynthesis of prenyl precursors for the production of primary and secondary metabolites, including sterols, dolichols, carotenoids and ubiquinones, and for the modification of proteins | Eucommia ulmoides |