Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Streptomyces roseochromogenus subsp. oscitans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 7 mM used in assay conditions | Streptomyces roseochromogenus subsp. oscitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces roseochromogenus subsp. oscitans | - |
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces roseochromogenus subsp. oscitans | Q8GHB2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Streptomyces roseochromogenus subsp. oscitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 3'-hydroxydaidzein | 2% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + 4'-hydroxy-6-methoxyflavone | 1% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone | 2% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone | 2% conversion | Streptomyces roseochromogenus subsp. oscitans | diphosphate + 2-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-7-methoxy-4H-chromen-4-one + 7-methoxy-2-[4-[(3-methylbut-2-en-1-yl)oxy]phenyl]-4H-chromen-4-one | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | - |
Streptomyces roseochromogenus subsp. oscitans | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | 84% conversion | Streptomyces roseochromogenus subsp. oscitans | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 7,4'-dihydroxyflavone | 4% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + coumestrol | 1% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + daidzein | less than 1% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + equol | 8% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + equol | 2% conversion | Streptomyces roseochromogenus subsp. oscitans | diphosphate + 3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3,4-dihydro-2H-chromen-7-ol + 3-(4-hydroxyphenyl)-6-(3-methylbut-2-en-1-yl)-3,4-dihydro-2H-chromen-7-ol | - |
? | |
dimethylallyl diphosphate + genistein | less than 1% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + luteolin | 3% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
dimethylallyl diphosphate + resveratrol | 2% conversion | Streptomyces roseochromogenus subsp. oscitans | ? | - |
? | |
additional information | the enzyme shows no activity with isoflavone and glycitein | Streptomyces roseochromogenus subsp. oscitans | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Streptomyces roseochromogenus subsp. oscitans |
Synonyms | Comment | Organism |
---|---|---|
CloQ | - |
Streptomyces roseochromogenus subsp. oscitans |