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Information on EC 2.5.1.111 - 4-hydroxyphenylpyruvate 3-dimethylallyltransferase
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2.5.1.111 4-hydroxyphenylpyruvate 3-dimethylallyltransferaseIUBMB Comments
The enzyme's product feeds into the biosynthesis of the aminocoumarin antibiotics clorobiocin and novobiocin .
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Word Map
- 2.5.1.111
-
geranylation
-
isoprenylation
-
dmapp
- dimethylallyltryptophan
- clorobiocin
-
isonitrile
-
hapalindole-type
-
aptase
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
aromatic prenyltransferase, isoprenyl transferase, famd1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
4HPP dimethylallyltransferase
apT
aromatic prenyltransferase
CloQ
FamD1
NovQ
CloQ
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate = diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl diphosphate:4-hydroxyphenylpyruvate 3-dimethylallyltransferase
The enzyme's product feeds into the biosynthesis of the aminocoumarin antibiotics clorobiocin and novobiocin [1].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene
diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene
dimethylallyl diphosphate + 3'-hydroxydaidzein
?
2% conversion
-
-
?
dimethylallyl diphosphate + 4'-hydroxy-6-methoxyflavone
?
1% conversion
-
-
?
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone
?
2% conversion
-
-
?
dimethylallyl diphosphate + 4'-hydroxy-7-methoxyflavone
diphosphate + 2-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-7-methoxy-4H-chromen-4-one + 7-methoxy-2-[4-[(3-methylbut-2-en-1-yl)oxy]phenyl]-4H-chromen-4-one
2% conversion
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
dimethylallyl diphosphate + 7,4'-dihydroxyflavone
?
4% conversion
-
-
?
dimethylallyl diphosphate + apigenin
diphosphate + 3'-C-dimethylallyl apigenin
7.6% yield
-
-
?
dimethylallyl diphosphate + caffeate
diphosphate + 3-O,4-O-bis-(dimethylallyl)caffeate
20% yield
-
-
?
dimethylallyl diphosphate + caffeate
diphosphate + 3-O-(dimethylallyl)caffeate
17.3% yield
-
-
?
dimethylallyl diphosphate + caffeate
diphosphate + 4-O-(dimethylallyl)caffeate
26.1% yield
-
-
?
dimethylallyl diphosphate + coumestrol
?
1% conversion
-
-
?
dimethylallyl diphosphate + daidzein
?
less than 1% conversion
-
-
?
dimethylallyl diphosphate + equol
?
8% conversion
-
-
?
dimethylallyl diphosphate + equol
diphosphate + 3-[4-hydroxy-3-(3-methylbut-2-en-1-yl)phenyl]-3,4-dihydro-2H-chromen-7-ol + 3-(4-hydroxyphenyl)-6-(3-methylbut-2-en-1-yl)-3,4-dihydro-2H-chromen-7-ol
2% conversion
-
-
?
dimethylallyl diphosphate + genistein
?
less than 1% conversion
-
-
?
dimethylallyl diphosphate + luteolin
?
3% conversion
-
-
?
dimethylallyl diphosphate + naringenin
diphosphate + 3'-C-dimethylallyl naringenin
87.2% yield
-
-
?
dimethylallyl diphosphate + naringenin
diphosphate + 4'-O-dimethylallyl naringenin
11.1% yield
-
-
?
dimethylallyl diphosphate + resveratrol
?
2% conversion
-
-
?
dimethylallyl diphosphate + resveratrol
diphosphate + 3'-dimethylallyl resveratrol
12.3% yield
-
-
?
geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile
dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile
dimethylallyl diphosphate is preferred over geranyl diphosphate
-
-
?
dimethylallyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2E)-3-[2-(3-methylbut-2-en-1-yl)-1H-indol-3-yl]prop-2-enenitrile + (2E)-3-[2-(3-methylbut-2-en-1-yl)-3H-indol-3-yl]prop-2-enenitrile
dimethylallyl diphosphate is preferred over geranyl diphosphate
-
-
?
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene
diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene
49.8% yield
-
-
?
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene
diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene
49.8% yield
-
-
?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene
31% yield
-
-
?
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene
diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene
31% yield
-
-
?
dimethylallyl diphosphate + 4-coumarate
diphosphate + 4-O-dimethylallylcoumarate
16.8% yield
-
-
?
dimethylallyl diphosphate + 4-coumarate
diphosphate + 4-O-dimethylallylcoumarate
34.4% yield
-
-
?
dimethylallyl diphosphate + 4-coumarate
diphosphate + 4-O-dimethylallylcoumarate
16.8% yield
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
Streptomyces niveus CPCC 240348
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic clorobiocin, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
84% conversion
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed
-
-
?
dimethylallyl diphosphate + hapalindole U
diphosphate + ambiguine H
-
-
-
?
dimethylallyl diphosphate + hapalindole U
diphosphate + ambiguine H
-
-
-
?
dimethylallyl diphosphate + menadione hydroquinol
diphosphate + vitamin MK1
-
-
-
?
dimethylallyl diphosphate + menadione hydroquinol
diphosphate + vitamin MK1
Streptomyces niveus CPCC 240348
-
-
-
?
geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile
dimethylallyl diphosphate is preferred over geranyl diphosphate
-
-
?
geranyl diphosphate + (2Z)-3-(1H-indol-3-yl)prop-2-enenitrile
diphosphate + (2Z)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1H-indol-3-yl)prop-2-enenitrile + (2E)-3-(2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-3H-indol-3-yl)prop-2-enenitrile
dimethylallyl diphosphate is preferred over geranyl diphosphate
-
-
?
additional information
?
-
no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene
-
-
?
additional information
?
-
no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene
-
-
?
additional information
?
-
the enzyme shows no activity with isoflavone and glycitein
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
Streptomyces niveus CPCC 240348
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic clorobiocin, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
-
-
?
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
the addition of Ca2+ (2.5 mM) instead of Mg2+ results in 70% of the activity obtained with Mg2+
Mg2+
additional information
Mg2+
activates, 2.5 mM is the most effecive concentration. In the absence of divalent cations and in the presence of 5 mM EDTA, the enzyme retains 25% of its original activity. The enzyme is not dependent on the presence of Mg2+
additional information
Mg2+-independent enzyme
additional information
both the zinc and the magnesium contents are below 0.1 mol per mol of CloQ
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
menadione hydroquinol
the relative activity is drastically decreased when the reaction system is supplemented with more than 1.5 mM of menadione hydroquinol. 3 mM menadione hydroquinol almost absolutely inhibits the prenylation by precipitating the enzyme in the reaction system
additional information
even in the presence of 5mM EDTA, NovQ possessed full enzymatic activity
-
additional information
nearly full activity is obtained in the presence of 10 mM EDTA
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Coma
ComQXPA quorum sensing systems may not be unique to Bacillus subtilis: a census in prokaryotic genomes.
Neoplasms
Antroquinonol blocks Ras and Rho signaling via the inhibition of protein isoprenyltransferase activity in cancer cells.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.016
4-hydroxyphenylpyruvate
pH 7.5, 30°C, wild-type enzyme
0.025
4-hydroxyphenylpyruvate
pH 7.5, 30°C
0.025
4-hydroxyphenylpyruvate
pH 7.5, 30°C, C215 mutant enzyme
0.025
dimethylallyl diphosphate
pH 7.5, 30°C, wild-type
0.035
dimethylallyl diphosphate
pH 7.5, 30°C
0.1044
menadione hydroquinol
mutant enzyme E281R, at pH 8.0 and 32°C
0.1537
menadione hydroquinol
mutant enzyme E281Q, at pH 8.0 and 32°C
0.2106
menadione hydroquinol
mutant enzyme E281M, at pH 8.0 and 32°C
0.2181
menadione hydroquinol
mutant enzyme E281K, at pH 8.0 and 32°C
0.2933
menadione hydroquinol
mutant enzyme E281V, at pH 8.0 and 32°C
0.3623
menadione hydroquinol
mutant enzyme E281A, at pH 8.0 and 32°C
0.4097
menadione hydroquinol
mutant enzyme E281S, at pH 8.0 and 32°C
0.4799
menadione hydroquinol
mutant enzyme E281T, at pH 8.0 and 32°C
0.5202
menadione hydroquinol
mutant enzyme E281D, at pH 8.0 and 32°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.151
4-hydroxyphenylpyruvate
pH 7.5, 30°C, C215 mutant enzyme
0.159
4-hydroxyphenylpyruvate
pH 7.5, 30°C, wild-type enzyme
0.253
dimethylallyl diphosphate
pH 7.5, 30°C, wild-type
0.003
menadione hydroquinol
mutant enzyme E281R, at pH 8.0 and 32°C
0.005
menadione hydroquinol
mutant enzyme E281A, at pH 8.0 and 32°C
0.014
menadione hydroquinol
mutant enzyme E281S, at pH 8.0 and 32°C
0.022
menadione hydroquinol
mutant enzyme E281K, at pH 8.0 and 32°C
0.026
menadione hydroquinol
mutant enzyme E281T, at pH 8.0 and 32°C
0.038
menadione hydroquinol
mutant enzyme E281V, at pH 8.0 and 32°C
0.039
menadione hydroquinol
mutant enzyme E281M, at pH 8.0 and 32°C
0.045
menadione hydroquinol
mutant enzyme E281D, at pH 8.0 and 32°C
0.083
menadione hydroquinol
mutant enzyme E281Q, at pH 8.0 and 32°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10.12
dimethylallyl diphosphate
pH 7.5, 30°C, wild-type
6.04
4-hydroxyphenylpyruvate
pH 7.5, 30°C, C215 mutant enzyme
9.94
4-hydroxyphenylpyruvate
pH 7.5, 30°C, wild-type enzyme
0.014
menadione hydroquinol
mutant enzyme E281A, at pH 8.0 and 32°C
0.029
menadione hydroquinol
mutant enzyme E281R, at pH 8.0 and 32°C
0.054
menadione hydroquinol
mutant enzyme E281T, at pH 8.0 and 32°C
0.064
menadione hydroquinol
mutant enzyme E281S, at pH 8.0 and 32°C
0.087
menadione hydroquinol
mutant enzyme E281D, at pH 8.0 and 32°C
0.13
menadione hydroquinol
mutant enzyme E281V, at pH 8.0 and 32°C
0.185
menadione hydroquinol
mutant enzyme E281M, at pH 8.0 and 32°C
0.54
menadione hydroquinol
mutant enzyme E281Q, at pH 8.0 and 32°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
cloQ-defective mutant of the clorobiocin producer Streptomyces roseochromogenes, which is blocked in the biosynthesis of the prenylated 4-hydroxybenzoic acid moiety of clorobiocin produces three new antibiotics, vanillobiocin, isovanillobiocin and declovanillobiocin. The isolated compounds are similar to clorobiocin, but contain vanillic acid as the acyl component instead of the prenylated 4-hydroxybenzoic acid present in clorobiocin. Isovanillobiocin differs from vanillobiocin by the position of the pyrrole unit attached to the sugar moiety of the antibiotic. Declovanillobiocin lacks the chlorine atom at the aminocoumarin ring. All three compounds have lower antibiotic activity against Bacillus subtilis than clorobiocin
physiological function
physiological function
involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic clorobiocin, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate
physiological function
the enzyme is involved in the biosynthesis of the 4-hydroxy-3-dimethylallylphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
physiological function
-
the enzyme is involved in the biosynthesis of the 4-hydroxy-3-dimethylallylphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CLOQ_STRRC
324
0
35626
Swiss-Prot
-
A0A1J0AKI6_FISAU
Fischerella ambigua (strain UTEX 1903)
343
0
39012
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M imidazole, pH 8.0
crystals are grown by hanging-drop vapor diffusion at 20 °C. Structure of of the apoenzyme, of CloQ complexed with 4-hydroxyphenylpyruvate, of mutant enzyme C215S complexed with 4-hydroxyphenylpyruvate and of the mutant enzyme R66S complexed with 4-hydroxyphenylpyruvate. Structure of CloQ complexed with 4-hydroxyphenylpyruvate reveals the formation of a covalent link between the substrate and Cys215 to yield a thiohemiketal species
crystals are grown by vapour diffusion. The protein crystallizes in space group I4(1)22, with unit-cell parameters a = b = 135.19, c = 98.13 A. Native data from a single crystal are recorded to a resolution of 2.2 A in-house
molecular mechanics and hybrid quantum mechanics/molecular mechanics molecular dynamics simulations. Positively charged basic residues line the inside of the beta-barrel of CloQ to activate the diphosphate leaving group to replace the function of the Mg(2+) cofactor in other APTases. The effect of the replacement of the Mg(2+) cofactor with basic residues yields a similar activation barrier for prenylation to Mg(2+)-dependent APTases like NphB. The topology of the binding pocket for 4-hydroxyphenylpyruvate is important for selective prenylation at the ortho position of the ring. Methylation at this position alters the conformation of the substrate for O-prenylation at the phenol group
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E281A
the mutant shows about 10% activity compared to the wild type enzyme
E281D
the mutant shows about 78% activity compared to the wild type enzyme
E281K
the mutant shows about 60% activity compared to the wild type enzyme
E281M
the mutant shows about 98% activity compared to the wild type enzyme
E281Q
the mutant shows 205% activity compared to the wild type enzyme
E281R
the mutant shows about 5% activity compared to the wild type enzyme
E281S
the mutant shows about 30% activity compared to the wild type enzyme
E281T
the mutant shows about 40% activity compared to the wild type enzyme
E281V
the mutant shows about 78% activity compared to the wild type enzyme
E281A
Streptomyces niveus CPCC 240348
-
the mutant shows about 10% activity compared to the wild type enzyme
-
E281D
Streptomyces niveus CPCC 240348
-
the mutant shows about 78% activity compared to the wild type enzyme
-
E281K
Streptomyces niveus CPCC 240348
-
the mutant shows about 60% activity compared to the wild type enzyme
-
E281Q
Streptomyces niveus CPCC 240348
-
the mutant shows 205% activity compared to the wild type enzyme
-
E281R
Streptomyces niveus CPCC 240348
-
the mutant shows about 5% activity compared to the wild type enzyme
-
C215A
activity is comparable to that of the wild-type enzyme
C215S
activity is comparable to that of the wild-type enzyme
E281G
R160A
mutant enzyme shows 17.3% of wild-type activity
R160Q
mutant enzyme shows 19.7% of wild-type activity
R66S
mutant enzyme shows 1.9% of wild-type activity
E281G
mutant enzyme shows 1% of wild-type activity
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) pLysS cells
overexpressed in Escherichia coli as an N-terminal His8-tagged protein
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme expression increases by 36% when 2% (v/v) methanol is added every 24 h
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
NovQ is capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids
synthesis
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NovQ is capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids
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REF.
AUTHORS
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