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Literature summary for 2.4.3.3 extracted from

  • Sherblom, A.P.; Bourassa, C.R.
    Specificity of submaxillary gland sialyltransferases (1983), Biochim. Biophys. Acta, 761, 94-102.
    View publication on PubMed
Search for more literature for 2.4.3.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 CMP-N-acetylneuraminate
-
 Ovis aries
2.2
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
2.7
-
 CMP-N-acetylneuraminate
-
 Bos taurus
2.8
-
 CMP-N-acetylneuraminate
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Sus scrofa the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Bos taurus the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Ovis aries the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Ovis aries
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
submaxillary gland
-
 Rattus norvegicus
-
submaxillary gland
-
 Sus scrofa
-
submaxillary gland
-
 Bos taurus
-
submaxillary gland
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003 0.005 crude extract, acceptor asialo-fetuin, pH 7.1, 37°C Rattus norvegicus
0.0003 0.005 crude extract, acceptor asialo-fetuin, pH 7.1, 37°C Sus scrofa
0.0003 0.005 crude extract, acceptor asialo-fetuin, pH 7.1, 37°C Bos taurus
0.0003 0.005 crude extract, acceptor asialo-fetuin, pH 7.1, 37°C Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-fetuin formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc Rattus norvegicus CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-fetuin formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc Sus scrofa CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-fetuin formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc Bos taurus CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-fetuin formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc Ovis aries CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis Sus scrofa CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis Bos taurus CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis Ovis aries CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + lactose poor acceptor Rattus norvegicus CMP + 6'-sialyllactose
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein preference of enzyme for a monosaccharide versus a disaccharide acceptor Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein preference of enzyme for a monosaccharide versus a disaccharide acceptor Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein preference of enzyme for a monosaccharide versus a disaccharide acceptor Ovis aries CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Rattus norvegicus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Ovis aries CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Ovis aries CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + submaxillary asialo-mucin ovine submaxillary asialo-mucin Sus scrofa CMP + submaxillary mucin formation of NeuAcalpha(2-6)GalNAc ?
CMP-N-acetylneuraminate + submaxillary asialo-mucin ovine submaxillary asialo-mucin Bos taurus CMP + submaxillary mucin formation of NeuAcalpha(2-6)GalNAc ?
CMP-N-acetylneuraminate + submaxillary asialo-mucin ovine submaxillary asialo-mucin Ovis aries CMP + submaxillary mucin formation of NeuAcalpha(2-6)GalNAc ?
additional information no acceptor: lactose Sus scrofa ?
-
 ?
additional information no acceptor: lactose Bos taurus ?
-
 ?
additional information no acceptor: lactose Ovis aries ?
-
 ?
additional information very poor acceptor: ovine submaxillary asialo-mucin Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus
37
-
 assay at Sus scrofa
37
-
 assay at Bos taurus
37
-
 assay at Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
 acceptor: asialo-fetuin Sus scrofa
7.1
-
 acceptor: asialo-fetuin Bos taurus
7.1
-
 acceptor: asialo-fetuin Ovis aries
7.5
-
 acceptor: asialo-fetuin Rattus norvegicus