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Information on EC 2.4.3.3 - alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase
for references in articles please use BRENDA:EC2.4.3.3
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.3 Sialyltransferases
2.4.3.3 alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase
IUBMB Comments
N-acetyl-α-D-galactosamine linked to threonine or serine is also an acceptor, when substituted at the 3-position.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
showSynonyms
sialyltransferase, st6gal-i, st6galnac i, st6galnac-i, alpha-2,6-st, st6galnac-ii, st6galnaci, alpha-n-acetylgalactosaminide alpha-2,6-sialyltransferase, sialyltransferase 7a, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-2,6-ST
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alpha-N-acetylgalactosaminylprotein alpha2-->6 sialyltransferase
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alpha2,6-sialyltransferase
CMP-N-acetylneuraminate:alpha-N-acetylgalactosaminide alpha2-->6 sialyltransferase
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CMP-Neu5Ac:GalNAc alpha2,6-sialyltransferase
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CMP-NeuAc:galactoside (alpha2-6)-sialyltransferase
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CMP-sialic acid:alpha-N-acetylgalactosaminide(R-->Galbeta1-->3GalNAcalpha1-->O-Ser/Thr) alpha2-->6 sialyltransferase
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mucin sialyltransferase
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sialyltransferase
sialyltransferase 7A
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sialyltransferase, cytidine monophosphoacetylneuraminate-alpha-acetylgalactosaminide alpha2-->6-
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ST6
ST6GalNAc-I
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ST6GalNAc-II
ST6GalNAcI
ST6GalNAc-II
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein
mechanism
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CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein alpha-2,6-N-acetylneuraminyltransferase
N-acetyl-alpha-D-galactosamine linked to threonine or serine is also an acceptor, when substituted at the 3-position.
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CAS REGISTRY NUMBER
COMMENTARY
71124-50-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP-9-acetamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-acetamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: less effective than CMP-N-acetylneuraminate
Products: -
Products: -
?
CMP-9-aminoacetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-aminoacetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-9-aminoacetylneuraminate can replace CMP-N-acetylneuraminate only with asialofetuin as acceptor
Products: -
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?
CMP-9-azido-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-azido-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-9-azido-N-acetylneuraminate can replace CMP-N-acetylneuraminate with asialofetuin or antifreeze glycoprotein as acceptor
Products: -
Products: -
?
CMP-9-benzamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-benzamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: less effective than CMP-N-acetylneuraminate
Products: -
Products: -
?
CMP-9-hexanoylamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-hexanoylamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: less effective than CMP-N-acetylneuraminate
Products: -
Products: -
?
CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-N-acetyl-beta-neuraminate + asialofetuin
CMP + fetuin
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Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + N-acetyllactosamin-butyl
CMP + ?
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Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + recombinant alpha-1-antitrypsin
CMP + ?
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Substrates: in addition to the expected alpha-2,6-sialylation of galactose, a second disialyl galactose motif Neu5Ac-alpha2,3(Neu5Ac-alpha2,6)Gal is produced by the enzyme activity
Products: -
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?
CMP-N-acetylneuraminate + asialo-milk glycopeptide
CMP + milk glycopeptide
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Substrates: -
Products: -
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?
CMP-N-acetylneuraminate + asialo-orosomucoid
CMP + orosomucoid
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-polysialoglycoprotein
CMP + alpha-sialosyl-(2-6)-asialo-polysialoglycoprotein
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Substrates: from egg, Sia residue is alpha-2,6-linked to the proximal GalNAc residue in asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr
Products: -
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?
CMP-N-acetylneuraminate + erythrocyte hemagglutination inhibitor
?
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: -
Products: -
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?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-N-acetylneuraminate + GTTPSPVPT[GalNAc]TSTTSAP
CMP + GTTPSPVPT[Neu5Acalpha(2-6)GalNAc]TSTTSAP
Substrates: hexadecaglycopeptide acceptor, corresponding to the MUC5AC tandem repeat sequence substituted by a GalNAc residue on Thr-9, hST6GalNAc-I
Products: -
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?
CMP-N-acetylneuraminate + lactose
CMP + 6'-sialyllactose
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Substrates: poor acceptor
Products: -
Products: -
?
CMP-N-acetylneuraminate + native polysialoglycoprotein
?
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Substrates: from egg, almost identical amount of Neu5Ac is incorporated into native and asialo-polysialoglycoprotein acceptor, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr
Products: -
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?
CMP-N-acetylneuraminate + NeuAcalpha(2-3)Galbeta(1-3)GalNAc-R
CMP + NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
CMP-N-acetylneuraminate + p-nitrophenyl-beta-D-galactoside
CMP + alpha-sialosyl-(2-6)-p-nitrophenyl-beta-D-galactoside
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Substrates: -
Products: -
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?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-N-acetylneuraminate + VPSTPPTPSPSTPPTPSPSCCHPR-GalNAc
CMP + ?
A0A024R8M1
Substrates: -
Products: -
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?
CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-Neu5Ac + fetuin
CMP + ?
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Substrates: isoform ST6GalNAc-I, 84% of reactivity with asialofetuin, isoform ST6GalNAc-II, 88% of reactivity with asialofetuin
Products: -
Products: -
?
CMP-Neu5Ac + HIV 120gp (17-mer)
CMP + ?
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Substrates: isoform ST6GalNAc-I, 34% of reactivity with asialofetuin, isoform ST6GalNAc-II, 38% of reactivity with asialofetuin
Products: -
Products: -
?
CMP-Neu5Ac + HIV 120gp (21-mer)
CMP + ?
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Substrates: isoform ST6GalNAc-I, 62% of reactivity with asialofetuin, isoform ST6GalNAc-II, 58% of reactivity with asialofetuin
Products: -
Products: -
?
CMP-sialic acid-PEG4-biotin + desialyated bovine serum albumin
?
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Substrates: bacterially produced ST6 is active and capable of catalyzing the transfer of sialic acid onto a desialylated O-glycoprotein, bovine submaxillary mucin
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?
cytidine-5'-yl-5-(acetamido)-9-(3-[4-chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate + asialofetuin
CMP + ?
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Substrates: CMP-Neu9NSydCl
Products: -
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?
cytidine-5'-yl-5-(acetamido)-9-(3-[4-chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate + butyl beta-N-acetyllactosaminide
CMP + ?
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Substrates: CMP-Neu9NSydCl
Products: -
Products: -
?
cytidine-5'-yl-5-(acetamido)-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate + asialofetuin
CMP + ?
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Substrates: CMP-Neu9Az
Products: -
Products: -
?
cytidine-5'-yl-5-(acetamido)-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate + N-acetyllactosamin-butyl
CMP + ?
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Substrates: CMP-Neu9Az
Products: -
Products: -
?
CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: poor donor, 10% as effective as CMP-N-acetylneuraminate
Products: -
Products: -
?
CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: poor donor, 10% as effective as CMP-N-acetylneuraminate
Products: -
Products: -
?
CMP-N-acetylneuraminate + antifreeze glycoprotein
?
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Substrates: from antarctic fish
Products: -
Products: -
r
CMP-N-acetylneuraminate + antifreeze glycoprotein
?
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Substrates: antifreeze glycoprotein 8, best acceptor
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: ST6GalNAc-II
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
Substrates: STM, i.e. hST6GalNAc-II, more effective than native fetuin
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: good substrate
Products: -
Products: -
?
CMP-N-acetylneuraminate + asialo-fetuin
CMP + fetuin
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Substrates: formation of Galbeta(1-3)(NeuAcalpha(2-6))GalNAc
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, formation of TF Sia6Core-1, Galbeta(1-3)(NeuAcalpha(2,6))GalNAc-R, or of TF DiSiaCore-1, NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2,6))GalNAc-R, by the sialyltransferase ST6GalNAc-II
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, but not p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + Galbeta(1-3)GalNAc-R
CMP + Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, but not p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + GalNAc-R
CMP + NeuAcalpha(2-6)GalNAc-R
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Substrates: formation of the TF-related blood group antigen sialosyl-Tn, NeuAcalpha(2-6)GalNAc-R, by the sialyltransferase ST6GalNAc-I
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?
CMP-N-acetylneuraminate + GalNAc-R
CMP + NeuAcalpha(2-6)GalNAc-R
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Substrates: R: protein
Products: -
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?
CMP-N-acetylneuraminate + GalNAc-R
CMP + NeuAcalpha(2-6)GalNAc-R
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Substrates: R: protein, but not p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + GalNAc-R
CMP + NeuAcalpha(2-6)GalNAc-R
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Substrates: R: protein
Products: -
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?
CMP-N-acetylneuraminate + GalNAc-R
CMP + NeuAcalpha(2-6)GalNAc-R
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Substrates: R: protein, but not p-nitrophenol
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: one of the last steps of the biosynthesis of oligosaccharides of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ST6GalNAc-II expression is significantly increased in colorectal carcinomas in cases with metastases to lymph nodes along the vascular trunk, involved in biosynthesis of the TF glycotype
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ST6GalNAc-I is responsible for the synthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAc, synthesis of Sialyl-Tn structures in mucin O-glycosylation stops further processing and elongation of the carbohydrate chain
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: pathway of biosynthesis of glycans present in mucins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: involved in the sialylation of submaxillary gland mucin in vivo
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: last step in the synthesis of ovine submaxillary mucin, catalyzes the chain-terminating process in oligosaccharide synthesis of glycoproteins, may well be involved in the process of secretion
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: pathway of biosynthesis of glycans present in mucins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: involved in the biosynthesis of oligosaccharide sequences of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: one of the last steps of the biosynthesis of oligosaccharides of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: involved in biosynthesis of O-linked oligosaccharide chains of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + native fetuin
?
Substrates: STM, i.e. hST6GalNAc-II, 83% as effective as asialo-fetuin
Products: -
Products: -
?
CMP-N-acetylneuraminate + NeuAcalpha(2-3)Galbeta(1-3)GalNAc-R
CMP + NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, formation of TF Sia6Core-1, Galbeta(1-3)(NeuAcalpha(2,6))GalNAc-R, or of TF DiSiaCore-1, NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2,6))GalNAc-R, by the sialyltransferase ST6GalNAc-II
Products: -
Products: -
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CMP-N-acetylneuraminate + NeuAcalpha(2-3)Galbeta(1-3)GalNAc-R
CMP + NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, also p-nitrophenol, but with very low activity possibly due to another factor present in the fraction
Products: -
Products: -
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CMP-N-acetylneuraminate + NeuAcalpha(2-3)Galbeta(1-3)GalNAc-R
CMP + NeuAcalpha(2-3)Galbeta(1-3)(NeuAcalpha(2-6))GalNAc-R
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Substrates: R: protein, also p-nitrophenol, but with very low activity possibly due to another factor present in the fraction
Products: -
Products: -
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CMP-N-acetylneuraminate + protein MUC1-MFP6
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Substrates: recombinant protein substrate expressed in MDA-MB-231 breast cancer cells, glycoprofiling, overview
Products: -
Products: -
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CMP-N-acetylneuraminate + protein MUC1-MFP6
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Substrates: recombinant protein substrate, glycoprofiling, overview
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: preference of enzyme for a monosaccharide versus a disaccharide acceptor
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: antifreeze glycoprotein
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-orosomucoid
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ST6GalNAc-II demands an acceptor with a protein backbone
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: -
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: acceptor substrate specificity
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: hST6GalNAc-I transfers a sialic acid residue in alpha2,6-linkage to GalNAc-O-Ser/Thr, substrate specificity of hST6GalNAc-I and -II
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: Galbeta(1-3)GalNAc-O-Ser/Thr or Neu5Acalpha(2-3)Galbeta(1-3)GalNAc-O-Ser/Thr acceptor specificity
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: native fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: acceptor substrate specificity
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: native and asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: acceptor substrate specificity
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: preference of enzyme for a monosaccharide versus a disaccharide acceptor
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: mucin-type acceptors
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: erythrocyte hemagglutination inhibitor, milk glycopeptide
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: bovine submaxillary asialo-mucin
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: specific for alpha-N-acetylgalactosamine attached through alpha-glycosidic linkage to threonine or serine in polypeptide chain
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: porcine submaxillary asialo-mucin
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: porcine submaxillary asialo-mucin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: native submaxillary mucin, less effective than asialo-mucin
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: native fetuin
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: -
Products: -
ir
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: absolute requirement for protein as the aglycon
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: acceptor substrate specificity
Products: -
Products: -
r
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: acceptor substrate specificity
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: preference of enzyme for a monosaccharide versus a disaccharide acceptor
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: antifreeze glycoprotein is the best substrate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: sialyl-2,3-antifreeze glycoprotein, better than native form
Products: -
Products: -
r
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: antifreeze glycoprotein from antarctic fish
Products: -
Products: -
r
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: R: H or a beta-galactoside
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: asialo-fetuin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: transfers sialic acid to the core region of O-linked glycans
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: specific for alpha-N-acetylgalactosamine attached through alpha-glycosidic linkage to threonine or serine in polypeptide chain
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: porcine submaxillary asialo-mucin
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-9-acetamidoneuraminate, CMP-9-benzamidoneuraminate and CMP-9-hexanoylamidoneuraminate are less effective, CMP-9-azido-N-acetylneuraminate can replace CMP-N-acetylneuraminate with asialofetuin or antifreeze glycoprotein as acceptor, CMP-9-aminoacetylneuraminate only with asialofetuin as acceptor
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: antifreeze glycoprotein
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: -
Products: -
r
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: -
Products: -
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: absolute requirement for protein as the aglycon
Products: -
Products: -
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin
Products: formation of NeuAcalpha(2-6)GalNAc
Products: formation of NeuAcalpha(2-6)GalNAc
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: bovine submaxillary asialo-mucin
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
ir
CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: porcine submaxillary asialo-mucin
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
ir
CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin
Products: formation of NeuAcalpha(2-6)GalNAc
Products: formation of NeuAcalpha(2-6)GalNAc
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin is the most effective acceptor
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
ir
CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: porcine submaxillary asialo-mucin
Products: -
Products: -
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin
Products: -
Products: -
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine and porcine submaxillary asialo-mucin are good acceptors
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
Products: linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: ovine submaxillary asialo-mucin
Products: formation of NeuAcalpha(2-6)GalNAc
Products: formation of NeuAcalpha(2-6)GalNAc
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CMP-N-acetylneuraminate + submaxillary asialo-mucin
CMP + submaxillary mucin
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Substrates: porcine submaxillary asialo-mucin
Products: -
Products: -
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CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency
Products: -
Products: -
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CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: -
Products: -
Products: -
?
CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: -
Products: -
Products: -
?
CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Substrates: CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency
Products: -
Products: -
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additional information
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Substrates: STM contains 2 catalytic domains, the L-sialylmotif, aa 148-194, and S-sialylmotif, aa 303-324, no acceptors: asialo-BSM, alpha1 acid glycoprotein, glycosphingolipids
Products: -
Products: -
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additional information
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Substrates: STM contains 2 catalytic domains, the L-sialylmotif, aa 148-194, and S-sialylmotif, aa 303-324, no acceptors: asialo-BSM, alpha1 acid glycoprotein, glycosphingolipids
Products: -
Products: -
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additional information
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Substrates: no acceptor: TF-PAA, i.e. Galbeta(1-3)GalNAc conjugated to poly[N-(2-hydroxyethyl)acrylamide]
Products: -
Products: -
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additional information
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-
-
Substrates: enzyme transfers sialic acid to O-linked oligosaccharide of glycoprotein, isoform ST6GalNAc-I prefers sialyl Tn antigen, i.e. Neu5Acalpha2-6GalNAc-O-Ser/Thr, isoform ST6GalNAc-II prefers T antigen as substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme participates in the core O-glycan biosynthesis, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme transfers a sialic acid residue in alpha-2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure
Products: -
Products: -
?
additional information
?
-
-
Substrates: human ST6Gal-I demonstrates acceptor specificity towards various terminal LacNAc (type 2) structures including several glycoproteins containing these terminal structures. Significant activities towards GalNAcbeta1-4GlcNAc, i.e.LacDiNAc and GlcNAcbeta1-4GlcNAcbeta1-4GlcNAc, i.e. chitotriose structures are found and less activities with fucosides Fucalpha1-2Galbeta1-4GlcNAc. The enzyme also accepts chitobiose structures as (GlcNAcbeta1-2Manalpha)2,3-Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Gly-L-Glu-L-Asn. In addition, hST6Gal-I sialylates sulfated lactose and H-type 2 glycans
Products: -
Products: -
?
additional information
?
-
A0A024R8M1
Substrates: the enzyme can sialylate GalNAc of galactose-deficient immunoglobulin A1
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme can sialylate GalNAc of galactose-deficient immunoglobulin A1
Products: -
Products: -
?
additional information
?
-
-
Substrates: sialylation of Gal-deficient asialo-immunoglobulin A1 myeloma protein by the enzyme generates sialylated immunoglobulin A1 that mimics the Gal-deficient immunoglobulin A1 glycoforms in patients with IgA nephropathy, characterized by alpha2,6-sialylated galactose-deficient N-acetylgalactosamine. The enzyme adds three to six N-acetylneuraminic acid residues to synthetic hinge region peptides with three to seven N-acetylgalactosamine residues, with at least one N-acetylgalactosamine remaining without N-acetylneuraminic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with cytidine-5'-yl-5-(3-[4-Chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5-dideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate (CMP-Neu5SydCl)
Products: -
Products: -
?
additional information
?
-
-
Substrates: no acceptor: beta-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenol
Products: -
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?
additional information
?
-
-
Substrates: no acceptor: beta-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenol
Products: -
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?
additional information
?
-
-
Substrates: no acceptors: asialo-collacalia mucoid, asialo-proteose peptone, asialo-prothrombin, asialo-thyroglobulin, asialo-transferrin, asialo-chondromucoprotein fractions from cartilage, human blood group substance A or B, various sugars
Products: -
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?
additional information
?
-
-
Substrates: no acceptor: N-acetyl-D-galactosaminyl-p-nitrophenol
Products: -
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?
additional information
?
-
-
Substrates: very poor acceptor: ovine submaxillary asialo-mucin
Products: -
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?
additional information
?
-
-
Substrates: no acceptor: beta-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no donor: CMP-9-amino-acetylneuraminate
Products: -
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?
additional information
?
-
-
Substrates: no acceptor: human asialo-transferrin
Products: -
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?
additional information
?
-
-
Substrates: no acceptor: N-acetyl-D-galactosaminyl-p-nitrophenol
Products: -
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?
additional information
?
-
-
Substrates: no acceptors: glycoproteins with Asn-linked oligosaccharides, glycosides, mono- and oligosaccharides
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
CMP-N-acetylneuraminate + protein MUC1-MFP6
?
-
Substrates: recombinant protein substrate expressed in MDA-MB-231 breast cancer cells, glycoprofiling, overview
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: one of the last steps of the biosynthesis of oligosaccharides of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines
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Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: ST6GalNAc-II expression is significantly increased in colorectal carcinomas in cases with metastases to lymph nodes along the vascular trunk, involved in biosynthesis of the TF glycotype
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Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
Substrates: hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: ST6GalNAc-I is responsible for the synthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAc, synthesis of Sialyl-Tn structures in mucin O-glycosylation stops further processing and elongation of the carbohydrate chain
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis
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Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: pathway of biosynthesis of glycans present in mucins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: involved in the sialylation of submaxillary gland mucin in vivo
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: last step in the synthesis of ovine submaxillary mucin, catalyzes the chain-terminating process in oligosaccharide synthesis of glycoproteins, may well be involved in the process of secretion
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Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: pathway of biosynthesis of glycans present in mucins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: involved in the biosynthesis of oligosaccharide sequences of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: one of the last steps of the biosynthesis of oligosaccharides of glycoproteins
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: the relative preference of enzyme for a monosaccharide versus a substituted GalNAc may play a role in regulation of chain length during mucin synthesis
Products: -
Products: -
?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
Substrates: involved in biosynthesis of O-linked oligosaccharide chains of glycoproteins
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme participates in the core O-glycan biosynthesis, overview
Products: -
Products: -
?
additional information
?
-
A0A024R8M1
Substrates: the enzyme can sialylate GalNAc of galactose-deficient immunoglobulin A1
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme can sialylate GalNAc of galactose-deficient immunoglobulin A1
Products: -
Products: -
?
additional information
?
-
-
Substrates: sialylation of Gal-deficient asialo-immunoglobulin A1 myeloma protein by the enzyme generates sialylated immunoglobulin A1 that mimics the Gal-deficient immunoglobulin A1 glycoforms in patients with IgA nephropathy, characterized by alpha2,6-sialylated galactose-deficient N-acetylgalactosamine. The enzyme adds three to six N-acetylneuraminic acid residues to synthetic hinge region peptides with three to seven N-acetylgalactosamine residues, with at least one N-acetylgalactosamine remaining without N-acetylneuraminic acid
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
activity decreases with increasing divalent cation concentration
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Antifreeze glycoprotein
-
competitive to ovine submaxillary asialo-mucin, non-competitive to CMP-NeuAc
-
Triton X-100
-
above critical micelle concentration of 0.016%, 15% inhibition, reversible
additional information
-
not inhibited by EDTA; not inhibited by Mg2+; not inhibited by native ovine submaxillary mucin
-
additional information
-
not inhibited by EDTA; not inhibited by Mg2+; not inhibited by Zn2+, Mn2+
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
ST6 activity yield produced in Sf9 insect cells is higher as compared to bacterial cells
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.119
CMP-N-acetyl-beta-neuraminate
-
pH and temperature not specified in the publication
0.086
cytidine-5'-yl-5-(acetamido)-9-(3-[4-chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
0.385
cytidine-5'-yl-5-(acetamido)-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
0.52
CMP-N-acetylneuraminate
-
pH 6.5, 37°C, antifreeze glycoprotein 8 as acceptor
0.66
CMP-N-acetylneuraminate
-
pH 6.5, 37°C, ovine submaxillary asialo-mucin as acceptor
1.9
submaxillary asialo-mucin
-
ovine, calculated in terms of N-acetylgalactosamine acceptor sites, 37°C
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.183
CMP-N-acetyl-beta-neuraminate
-
pH and temperature not specified in the publication
0.0167
cytidine-5'-yl-5-(acetamido)-9-(3-[4-chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
0.3
cytidine-5'-yl-5-(acetamido)-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.53
CMP-N-acetyl-beta-neuraminate
-
pH and temperature not specified in the publication
1.93
cytidine-5'-yl-5-(acetamido)-9-(3-[4-chloro-1,2,3-oxadiazol-3-ium-5-olate]benzamido)-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
0.78
cytidine-5'-yl-5-(acetamido)-9-azido-3,5,9-trideoxy-beta-D-glycero-D-galacto-non-2-ulopyranosid-2''-yl phosphate
-
pH and temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0003 - 0.005
0.00435
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.1
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
malignant hematopoietic cell. Sialylated histo-blood group carbohydrate CD175 is present in all hematopoietic cell lines and native lymphocytes examined. CD175 and sialylated CD175 are preferentially expressed on erythroblastic leukemia cell lines. Presence of sialylated CD175 is consistent with the expression of the gene encoding alpha2,6-sialyltransferase ST6GalNAc-1
brenda
-
sialylated histo-blood group carbohydrate CD175 is present in all hematopoietic cell lines and native lymphocytes examined. CD175 and sialylated CD175 are preferentially expressed on erythroblastic leukemia cell lines. Presence of sialylated CD175 is consistent with the expression of the gene encoding alpha2,6-sialyltransferase ST6GalNAc-1
brenda
normal human mammary epithelial cell strains 70N, 76N and 81N, STM RNA expression is highest in 76N, followed by 81N and 70N
brenda
additional information
additional information
-
ratio of ST6GalNAc-II expression in colorectal tumor cells versus normal mucosa cells is 1.47
brenda
additional information
STM tissue distribution, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines, STM gene is down-regulated in T-47D, BT-474 and MDA-MB-435 breast tumor cell lines as compared to normal mammary epithelial cell strains, only RNA traces in other tumor cell lines tested, e.g. MDA-231, MDA-468 and MCF-7, only MDA-MB-361 metastatic tumor cell line expresses STM mRNA levels as high as in 76N
brenda
additional information
-
STM tissue distribution, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines, STM gene is down-regulated in T-47D, BT-474 and MDA-MB-435 breast tumor cell lines as compared to normal mammary epithelial cell strains, only RNA traces in other tumor cell lines tested, e.g. MDA-231, MDA-468 and MCF-7, only MDA-MB-361 metastatic tumor cell line expresses STM mRNA levels as high as in 76N
brenda
additional information
no expression of hST6GalNAc-I in MDA-MB-231, BT-20, MCF-7 and T47-D cells
brenda
additional information
-
low expression of ST6GalNAc-I in gastric carcinoma cell lines MKN45, KATO-III, GP202 and GP220, correlating with very low or absent detection of the Sialyl-Tn antigen
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles
-
brenda
-
in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles
-
brenda
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (2360 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100000 - 172000
-
enzyme exists in several active molecular weight forms from 100000-172000, gel filtration or PAGE
160000
-
x * 160000, enzyme consists of several electrophoretic forms, 160 kDa: largest one, SDS-PAGE
39000
-
x * 65000, isoform ST6GalNAc-I, x * 39000, isoform ST6GalNAc-II, SDS-PAGE
65000
65000
-
x * 65000, isoform ST6GalNAc-I, x * 39000, isoform ST6GalNAc-II, SDS-PAGE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 160000, enzyme consists of several electrophoretic forms, 160 kDa: largest one, SDS-PAGE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
amino acid sequence of STM has a potential site for casein kinase II phosphorylation and 3 consensus motifs for N-glycosylation at positions Asn-85, Asn-130 and Asn-161
additional information
-
amino acid sequence of STM has a potential site for casein kinase II phosphorylation and 3 consensus motifs for N-glycosylation at positions Asn-85, Asn-130 and Asn-161
additional information
-
no proteolytic cleavage at N-terminus of both isoform ST6GalNAc-I and ST6GalNAc-II
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin, 1 mg/ml, stabilizes purified enzyme
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM sodium cacodylate, pH 6.5, 50 mM NaCl, 50% glycerol, 0.02% NaN3, at least 6 months, stable
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a codon-optimized gene encoding the catalytic domain of ST6 (amino acids Lys36-end) cloned into pTrc99a downstream from the tac promoter, FLAG tag and an octahistidine tag fused at the N- and C-termini, respectively. Catalytic domain of ST6 cloned into the high-copy number plasmid pCWin2MBP, which expresses an N-terminal fusion of ST6 with the Escherichia coli maltose-binding protein under the control of a dual tac promoter. Expression of soluble ST6 in a variety of Escherichia coli strains with oxidizing cytoplasm. Fusion of the ST6 enzyme to maltose-binding protein expressed in Origami 2 cells
-
cDNA encoding the full-length hST6GalNAc-I from HT-29-MTX cells is cloned, stable transfection of MDA-MB-231 breast cancer cells expressing enzyme induces the expression of sialyl-Tn antigen at the cell surface associated with morphological changes, decreased growth and increased migration of the cells
SIATL1 gene encoding STM is mapped to the long arm of chromosome 17 at q23-qter, full-length cDNA from normal human mammary epithelial cell strain 76N is cloned, sequenced and encodes a protein of 374 amino acids, expression in COS-7 cells
ST6GalNAc-II cDNA is cloned, LS147T and SW-480 human colorectal carcinoma cells are transfected with human ST6GalNAc-II cDNA leading to an increased cell surface expression of Thomsen-Friedenreich-related blood group antigen TF, which depends in SW-480 cells on the ratio of core 2 beta-1,6-N-acetylglucosaminyltransferase and ST6GalNAc-II
-
stable transfection of the breast cancer cell line MDA-MB-231 with ST6GalNAc I cDNA induces sialyl-Tn antigen expression, modifications of the O-glycosylation pattern of a MUC1-related recombinant protein secreted by MDA-MB-231 sialyl-Tn positive cells occurs, sialyl-Tn expression and concomitant changes in the overall O-glycan profiles induce a decrease of adhesion and an increase of migration of MDA-MB-231, overview
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
while wild-type Escherichia coli strain DHB4 produces very small amounts of soluble ST6, engineered strains of Escherichia coli (strains SMG96, FA113, MJF277.2, and DR611), which possess certain types of oxidative cytoplasm or which co-express specific molecular chaperones (DnaK/DnaJ, GroEL/GroES, and Skp), can result in significant yields of ST6. DR611 cells are able to produce dramatically increased amounts of soluble ST6. Fusion of the ST6 enzyme to maltose-binding protein results in a marked increase in the amount of soluble ST6 in Origami 2 cells, as well as in the DR611 strain
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
-
Escherichia coli system may be used as a starting point for the evolution of sialyltransferases with better expression characteristics or altered donor/acceptor specificities
medicine
synthesis
-
use of glycan microarrays for high-throughput acceptor specificity screening of recombinant sialyltransferases. Donor substrate is cytidine 5'-monophospho-N-acteylneuraminate, which has been biotinylated at position 9. human alpha2,6sialyltransferase-I also sialylates chitobiose structures including N-glycans
medicine
-
St6GalNAc-II expression provides a prognostic factor for patient survival in human colorectal carcinomas, overexpression is related to poor patient survival
medicine
-
sialylated histo-blood group carbohydrate CD175 is present in all hematopoietic cell lines and native lymphocytes examined. CD175 and sialylated CD175 are preferentially expressed on erythroblastic leukemia cell lines. Presence of sialylated CD175 is consistent with the expression of the gene encoding alpha2,6-sialyltransferase ST6GalNAc-1
medicine
-
patients with IgA nephropathy display an aberrant glycosylation of IgA. EBV-immortalized IgA1-producing cells from these patients indicate a decrease in beta1,3-glucosyltransferase activity and an increase in N-acetylgalactosamine-specific alpha2,6-sialyltransferase activity, with concomitant increase in alpha2,6-sialyltransferase gene expression. EBV-immortalized cells from patients with lupus nephritis and healthy individuals do not produce IgA with the defective galactosylation pattern
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
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brenda
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brenda
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brenda
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brenda
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brenda
Sadler, J.E.; Rearick, J.I.; Paulson, J.C.; Hill, R.L.
Purification to homogeneity of a beta-galactoside alpha2 leads to 3 sialyltransferase and partial purification of an alpha-N-acetylgalactosaminide alpha2 leads to 6 sialyltransferase from porcine submaxillary glands
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brenda
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1989
Sus scrofa
brenda
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The sialic acids. XVI. Isolation of a mucin sialyltransferase from sheep submaxillary gland
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248
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1973
Ovis aries
brenda
Kitazume, S.; Kitajima, K.; Inoue, S.; Inoue, Y.; Troy, F.A.2nd.
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269
10330-10340
1994
Oncorhynchus mykiss
brenda
Schneider, F.; Kemmner, W.; Haensch, W.; Franke, G.; Gretschel, S.; Karsten, U.; Schlag, P.M.
Overexpression of sialyltransferase CMP-sialic acid:Galbeta1,3GalNAc-R alpha6-sialyltransferase is related to poor patient survival in human colorectal carcinomas
Cancer Res.
61
4605-4611
2001
Homo sapiens
brenda
Sotiropoulou, G.; Kono, M.; Anisowicz, A.; Stenman, G.; Tsuji, S.; Sager, R.
Identification and functional characterization of a human GalNAc alpha2,6-sialyltransferase with altered expression in breast cancer
Mol. Med.
8
42-55
2002
Homo sapiens (Q9UJ37), Homo sapiens
brenda
Julien, S.; Krzewinski-Recchi, M.A.; Harduin-Lepers, A.; Gouyer, V.; Huet, G.; Le Bourhis, X.; Delannoy, P.
Expression of sialyl-Tn antigen in breast cancer cells transfected with the human CMP-Neu5Ac:GalNAc alpha2,6-sialyltransferase (ST6GalNac I) cDNA
Glycoconjugate J.
18
883-893
2001
Homo sapiens (Q9NSC7)
-
brenda
Marcos, N.T.; Cruz, A.; Silva, F.; Almeida, R.; David, L.; Mandel, U.; Clausen, H.; von Mensdorff-Pouilly, S.; Reis, C.A.
Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I expression in gastric carcinoma cell lines
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51
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2003
Homo sapiens
brenda
Marcos, N.T.; Pinho, S.; Grandela, C.; Cruz, A.; Samyn-Petit, B.; Harduin-Lepers, A.; Almeida, R.; Silva, F.; Morais, V.; Costa, J.; Kihlberg, J.; Clausen, H.; Reis, C.A.
Role of the human ST6GalNAc-I and ST6GalNAc-II in the synthesis of the cancer-associated sialyl-Tn antigen
Cancer Res.
64
7050-7057
2004
Homo sapiens
brenda
Julien, S.; Adriaenssens, E.; Ottenberg, K.; Furlan, A.; Courtand, G.; Vercoutter-Edouart, A.S.; Hanisch, F.G.; Delannoy, P.; Le Bourhis, X.
ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumourigenicity
Glycobiology
16
54-64
2006
Homo sapiens
brenda
Blixt, O.; Allin, K.; Bohorov, O.; Liu, X.; Andersson-Sand, H.; Hoffmann, J.; Razi, N.
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25
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2008
Homo sapiens
brenda
Cao, Y.; Merling, A.; Karsten, U.; Goletz, S.; Punzel, M.; Kraft, R.; Butschak, G.; Schwartz-Albiez, R.
Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells
Int. J. Cancer
123
89-99
2008
Homo sapiens
brenda
Suzuki, H.; Moldoveanu, Z.; Hall, S.; Brown, R.; Vu, H.L.; Novak, L.; Julian, B.A.; Tomana, M.; Wyatt, R.J.; Edberg, J.C.; Alarcon, G.S.; Kimberly, R.P.; Tomino, Y.; Mestecky, J.; Novak, J.
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118
629-639
2008
Homo sapiens
brenda
Skretas, G.; Carroll, S.; DeFrees, S.; Schwartz, M.F.; Johnson, K.F.; Georgiou, G.
Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli
Microb. Cell Fact.
8
50
2009
Homo sapiens, Gallus gallus
brenda
Stuchlova Horynova, M.; Vrablikova, A.; Stewart, T.J.; Takahashi, K.; Czernekova, L.; Yamada, K.; Suzuki, H.; Julian, B.A.; Renfrow, M.B.; Novak, J.; Raska, M.
N-acetylgalactosaminide alpha2,6-sialyltransferase II is a candidate enzyme for sialylation of galactose-deficient IgA1, the key autoantigen in IgA nephropathy
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30
234-238
2015
Homo sapiens (A0A024R8M1), Homo sapiens
brenda
Takahashi, K.; Raska, M.; Horynova, M.; Hall, S.; Poulsen, K.; Kilian, M.; Hiki, Y.; Yuzawa, Y.; Moldoveanu, Z.; Julian, B.; Renfrow, M.; Novak, J.
Enzymatic sialylation of IgA1 O-glycans: Implications for studies of IgA nephropathy
PLoS ONE
9
e99026
2014
Homo sapiens
brenda
Chinoy, Z.S.; Bodineau, C.; Favre, C.; Moremen, K.W.; Duran, R.V.; Friscourt, F.
Selective engineering of linkage-specific alpha2,6-N-linked sialoproteins using sydnone-modified sialic acid bioorthogonal reporters
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58
4281-4285
2019
Homo sapiens
brenda
Pallister, E.G.; Choo, M.S.F.; Tai, J.N.; Leong, D.S.Z.; Tang, W.Q.; Ng, S.K.; Huang, K.; Marchesi, A.; Both, P.; Gray, C.; Rudd, P.M.; Flitsch, S.L.; Nguyen-Khuong, T.
Exploiting the disialyl galactose activity of alpha2,6-sialyltransferase from Photobacterium damselae to generate a highly sialylated recombinant alpha-1-antitrypsin
Biochemistry
59
3123-3128
2020
Photobacterium damselae
brenda
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