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Literature summary for 2.4.3.2 extracted from

  • Chandrasekaran, E.V.; Xue, J.; Xia, J.; Chawda, R.; Piskorz, C.; Locke, R.D.; Neelamegham, S.; Matta, K.L.
    Analysis of the specificity of sialyltransferases toward mucin core 2, globo, and related structures. Identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the biosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha2,3(O)sialyltransferase (2005), Biochemistry, 44, 15619-15635 .
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 methyl beta-D-Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal pH 6.0, 37°C, alpha2,3(O) sialyltransferase Rattus norvegicus
1.25
-
 methyl beta-D-(3-O-sulfo)Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal pH 6.0, 37°C, alpha2,3(O) sialyltransferase Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, 3 months, retains full activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 CMP-N-acetyl-beta-neuraminate + 2 methyl beta-D-(3-O-sulfo)Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal
-
 Rattus norvegicus 2 CMP + methyl alpha-Neu5Ac-(2->2)-beta-D-(3-O-sulfo)Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal + methyl alpha-Neu5Ac-(2->4)-beta-D-(3-O-sulfo)Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal
-
 ?
CMP-N-acetyl-beta-neuraminate + allyl beta-D-Gal-(1->3)-beta-D-GalNAc 24.1% of the activity compared to allylbeta-D-Gal-(1->4)-beta-D-GlcNAc, alpha2,3(N) sialyltransferase. 112% of the activity compared to benzyl beta-D-(3-O-Me)Gal-(1->4)-beta-D-GlcNAc-(1->6)-[beta-D-Gal-(1->3)]-alpha-D-GalNAc, alpha2,3(O) sialyltransferase Rattus norvegicus CMP + allyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
 ?
CMP-N-acetyl-beta-neuraminate + allyl beta-D-Gal-(1->3)-beta-D-GlcNAc 123.1% of the activity compared to allylbeta-D-Gal-(1->4)-beta-D-GlcNAc, alpha2,3(N) sialyltransferase. 42.1% of the activity compared to benzyl beta-D-(3-O-Me)Gal-(1->4)-beta-D-GlcNAc-(1->6)-[beta-D-Gal-(1->3)]-alpha-D-GalNAc, alpha2,3(O) sialyltransferase Rattus norvegicus CMP + allyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GlcNAc
-
 ?
CMP-N-acetyl-beta-neuraminate + beta-D-(2-O-Me)Gal-(1->3)-beta-D-GlcNAc 115.3% of the activity compared to allylbeta-D-Gal-(1->4)-beta-D-GlcNAc, alpha2,3(N) sialyltransferase. 2.9% of the activity compared to benzyl beta-D-(3-O-Me)Gal-(1->4)-beta-D-GlcNAc-(1->6)-[beta-D-Gal-(1->3)]-alpha-D-GalNAc, alpha2,3(O) sialyltransferase Rattus norvegicus CMP + alpha-Neu5Ac-(2->3)-beta-D-(2-O-Me)Gal-(1->3)-beta-D-GlcNAc
-
 ?
CMP-N-acetyl-beta-neuraminate + methyl beta-D-Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal 106.8% of the activity compared to allylbeta-D-Gal-(1->4)-beta-D-GlcNAc, alpha2,3(N) sialyltransferase. 120.8% of the activity compared to benzyl beta-D-(3-O-Me)Gal-(1->4)-beta-D-GlcNAc-(1->6)-[beta-D-Gal-(1->3)]-alpha-D-GalNAc, alpha2,3(O) sialyltransferase Rattus norvegicus CMP + methyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal
-
 ?

Synonyms

Synonyms Comment Organism
alpha2,3(N) sialyltransferase
-
 Rattus norvegicus
alpha2,3(N)ST
-
 Rattus norvegicus
alpha2,3(O) sialyltransferase
-
 Rattus norvegicus
alpha2,3(O)ST
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Rattus norvegicus