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Literature summary for 2.4.3.1 extracted from

  • Janesch, B.; Saxena, H.; Sim, L.; Wakarchuk, W.W.
    Comparison of alpha2,6-sialyltransferases for sialylation of therapeutic proteins (2019), Glycobiology, 29, 735-747 .
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10B cells Photobacterium sp. JT-ISH-224
expressed in Escherichia coli DH10B cells Helicobacter cetorum
expressed in Escherichia coli or HEK-293F cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00086
-
 CMP-N-acetyl-beta-neuraminate at pH 7.4 and 37°C Helicobacter cetorum
0.00146
-
 CMP-N-acetyl-beta-neuraminate at pH 7.4 and 37°C Homo sapiens
0.0852
-
 CMP-N-acetyl-beta-neuraminate at pH 7.4 and 37°C Photobacterium sp. JT-ISH-224

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78800
-
 fused to maltose-binding protein, gel filtration Helicobacter cetorum

Organism

Organism UniProt Comment Textmining
Helicobacter cetorum
-
-
-
Helicobacter cetorum MIT 00-7128
-
-
-
Homo sapiens P15907
-
-
Photobacterium sp. JT-ISH-224
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Helicobacter cetorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + asialo-alpha-1-antitrypsin
-
 Homo sapiens CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialo-alpha-1-antitrypsin
-
 Photobacterium sp. JT-ISH-224 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialo-alpha-1-antitrypsin
-
 Helicobacter cetorum CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialo-alpha-1-antitrypsin
-
 Helicobacter cetorum MIT 00-7128 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Homo sapiens CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Photobacterium sp. JT-ISH-224 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Helicobacter cetorum CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Helicobacter cetorum MIT 00-7128 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + human butyrylcholinesterase
-
 Homo sapiens CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + human butyrylcholinesterase
-
 Photobacterium sp. JT-ISH-224 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + human butyrylcholinesterase
-
 Helicobacter cetorum CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + human butyrylcholinesterase
-
 Helicobacter cetorum MIT 00-7128 CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + N-acetyl-D-lactosamine
-
 Homo sapiens ?
-
 ?
CMP-N-acetyl-beta-neuraminate + N-acetyl-D-lactosamine
-
 Photobacterium sp. JT-ISH-224 ?
-
 ?
CMP-N-acetyl-beta-neuraminate + N-acetyl-D-lactosamine
-
 Helicobacter cetorum ?
-
 ?
CMP-N-acetyl-beta-neuraminate + N-acetyl-D-lactosamine
-
 Helicobacter cetorum MIT 00-7128 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40600, calculated from amino acid sequence Homo sapiens
? x * 88300, fused to maltose-binding protein, calculated from amino acid sequence Photobacterium sp. JT-ISH-224
homodimer 2 * 36700, calculated from amino acid sequence Helicobacter cetorum

Synonyms

Synonyms Comment Organism
alpha2,6-sialyltransferase
-
 Photobacterium sp. JT-ISH-224
alpha2,6-sialyltransferase
-
 Helicobacter cetorum
alpha2,6-ST
-
 Photobacterium sp. JT-ISH-224
alpha2,6-ST
-
 Helicobacter cetorum
alpha2,6-ST I
-
 Homo sapiens
ST6Gal1
-
 Homo sapiens