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Literature summary for 2.4.3.1 extracted from

  • Kajiura, H.; Hamaguchi, Y.; Mizushima, H.; Misaki, R.; Fujiyama, K.
    Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active alpha2,6-sialyltransferase (2015), Glycobiology, 25, 1441-1453.
    View publication on PubMed
Search for more literature for 2.4.3.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 138% activity at 10 mM Bombyx mori

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Bombyx mori

Protein Variants

Protein Variants Comment Organism
N284A the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
N284Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
CTP complete inhibition at 10 mM Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 CMP-N-acetylneuraminate wild type enzyme, at pH 6.0 and 37°C Bombyx mori
1.18
-
 CMP-N-acetylneuraminate mutant enzyme N284Q, at pH 6.0 and 37°C Bombyx mori
2.02
-
 CMP-N-acetylneuraminate mutant enzyme N284A, at pH 6.0 and 37°C Bombyx mori
35.7
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl mutant enzyme N284Q, at pH 6.0 and 37°C Bombyx mori
40.2
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl wild type enzyme, at pH 6.0 and 37°C Bombyx mori
52.8
-
 Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl mutant enzyme N284A, at pH 6.0 and 37°C Bombyx mori

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Bombyx mori 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require ions for activity Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Bombyx mori
53100
-
 x * 53100, calculated from amino acid sequence Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Bombyx mori
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+ affinity column chromatography, and gel filtration Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Bombyx mori
-
larva
-
 Bombyx mori
-
malpighian tubule
-
 Bombyx mori
-
midgut
-
 Bombyx mori
-
silk gland
-
 Bombyx mori
-
testis
-
 Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + (Galbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc 34.9 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
CMP-N-acetylneuraminate + (GalNAcbeta(1,4))2(GlcNAcbeta(1,2))2(Man)3(GlcNAc)2-Asn-Fmoc 140 activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide 2.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactopyranosyl-(1->3)-N-acetyl-beta-D-glucosaminide
-
 ?
CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide 100% activity Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide
-
 ?
CMP-N-acetylneuraminate + 4-nitrophenyl N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide best acceptor with 371.5% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori CMP + 4-nitrophenyl N-acetyl-alpha-neuraminyl-(2->6)-N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminide
-
 ?
CMP-N-acetylneuraminate + asialofetuin
-
 Bombyx mori ?
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Bombyx mori CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + Galbeta-4-nitrophenyl 26.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
CMP-N-acetylneuraminate + GalNAcbeta-4-nitrophenyl 120.4% activity compared to Galbeta(1,4)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?
additional information no activity towards Glcalpha-4-nitrophenyl, Galalpha-4-nitrophenyl, GalNAcalpha-4-nitrophenyl, Galbeta(1,3)-GalNAcbeta-4-nitrophenyl and GalNAcbeta(1,3)-GlcNAcbeta-4-nitrophenyl Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Bombyx mori
? x * 53100, calculated from amino acid sequence Bombyx mori

Synonyms

Synonyms Comment Organism
alpha2,6-sialyltransferase
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bombyx mori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 35
-
 Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 30 the enzyme is stable below 30°C for 1 h but shows significantly decreased activities at higher temperatures Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Bombyx mori