Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.3.1 extracted from

  • Kuhn, B.; Benz, J.; Greif, M.; Engel, A.M.; Sobek, H.; Rudolph, M.G.
    The structure of human alpha-2,6-sialyltransferase reveals the binding mode of complex glycans (2013), Acta Crystallogr. Sect. D, 69, 1826-1838.
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
the truncated enzyme DELTA89 is expressed in HEK cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion, method truncated enzyme DELTA89 in complex with CMP using 0.1 M HEPES-NaOH pH 7.0, 20% (w/v) PEG 550 MME, 0.01 MCaCl2, 0.01 MMgCl2, or in complex with cytidine and phosphate using 0.1 M MES-NaOH pH 5.8, 20% (w/v) PEG 2000 MME, 0.01 M CaCl2, 0.01 M MgCl2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
 Homo sapiens
CTP
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Homo sapiens
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P15907
-
-

Purification (Commentary)

Purification (Comment) Organism
S-Sepharose column chromatography, heparin Sepharose column chromatography, and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-9-fluoresceinyl-N-acetylneuraminate + asialofetuin
-
 Homo sapiens ?
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Homo sapiens CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, truncated enzyme DELTA89, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-2,6-sialyltransferase
-
 Homo sapiens
beta-galactoside alpha-2,6-sialyltransferase I
-
 Homo sapiens
ST6Gal-I
-
 Homo sapiens