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Literature summary for 2.4.3.1 extracted from

  • Laporte, B.; Gonzalez-Hilarion, S.; Maftah, A.; Petit, J.M.
    The second bovine beta-galactoside-alpha2,6-sialyltransferase (ST6Gal II): genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells (2009), Glycobiology, 19, 1082-1093.
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
two pFlag-bST6Gal II vector constructions separately transfected in COS-1 cells for expression of either membrane-bound or soluble active forms of ST6Gal II Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.97
-
 GalNAcbeta(1,4)GlcNAc-octyl soluble protein, at 37°C Bos taurus
1.09
-
 GalNAcbeta(1,4)GlcNAc-octyl membrane-bound protein, at 37°C Bos taurus
2.04
-
 Galbeta(1,4)GlcNAc-octyl soluble protein, at 37°C Bos taurus
2.12
-
 Galbeta(1,4)GlcNAc-octyl membrane-bound protein, at 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol has a short cytosolic region of 11 amino acids (M1-G11) Bos taurus 5829
-
membrane is a type II membrane-bound protein with a short cytosolic region of 11 amino acids (M1-G11), a single transmembrane region of 19 amino acids (M12-F30) and a luminal domain of 465 amino acids (T31-A495) Bos taurus 16020
-

Organism

Organism UniProt Comment Textmining
Bos taurus A5D7T4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-
colon
-
 Bos taurus
-
lung high level Bos taurus
-
mammary epithelial cell
-
 Bos taurus
-
mammary gland low level Bos taurus
-
additional information is absent from thymus and the adipose tissue Bos taurus
-
salivary gland low level Bos taurus
-
skeletal muscle very low level Bos taurus
-
spleen
-
 Bos taurus
-
testis very low level Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialo-alpha1-acidglycoprotein + CMP-Neu5Ac membrane-bound and soluble protein, 34% and 25% relative activity, respectively Bos taurus ?
-
 ?
Galbeta(1,4)GlcNAc-octyl + CMP-Neu5Ac membrane-bound and soluble protein, 100% relative activity Bos taurus ?
-
 ?
GalNAcbeta(1,4)GlcNAc-octyl + CMP-Neu5Ac membrane-bound and soluble protein, 312% and 346% relative activity, respectively Bos taurus ?
-
 ?
additional information the enzyme uses the LacdiNAc structure (GalNAcbeta1,4GlcNAc) as a better acceptor substrate than the type II (Galbeta1-4GlcNAc) disaccharide. The enzyme's efficiency is improved when the acceptor substrate is provided as a free oligosaccharide rather than as a protein-bound oligosaccharide. No activity with alpha1-acidglycoprotein and Galbeta1–3GlcNAc-octyl Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-galactoside-alpha2,6-sialyltransferase
-
 Bos taurus
ST6Gal II
-
 Bos taurus

Expression

Organism Comment Expression
Bos taurus ST6Gal II gene is upregulated in cells stimulated by interleukin-6, whereas interleukin-1 and TNFalpha treatments have no effect on the expression of ST6Gal II. Inflammatory response induced by interleukin-6 treatment provokes an overexpression of ST6Gal II at 24 h and 48 h of treatment, its expression level in the treated cells is 2.5- and 5.6fold higher than in the control, respectively up

General Information

General Information Comment Organism
physiological function alpha2,6-sialylation mediated by ST6Gal II can contribute to organism's response to infections Bos taurus