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Literature summary for 2.4.3.1 extracted from

  • Koles, K.; Irvine, K.D.; Panin, V.M.
    Functional characterization of Drosophila sialyltransferase (2004), J. Biol. Chem., 279, 4346-4357.
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
a full-length Drosophila SiaT protein with a C-terminal HA tag is transiently expressed in Drosophila S2 cells. A fraction of SiaT-HA protein expressed in S2 cell culture is secreted into the cell medium in soluble form Drosophila sp. (in: flies)

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Drosophila sp. (in: flies)
Cu2+
-
 Drosophila sp. (in: flies)
Ni2+
-
 Drosophila sp. (in: flies)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Drosophila sp. (in: flies)
1.4
-
 Galbeta(1-4)GlcNAc non-reducing terminus of asialofetuin glycans Drosophila sp. (in: flies)

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Drosophila sp. (in: flies) 5794
-

Organism

Organism UniProt Comment Textmining
Drosophila sp. (in: flies)
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
sialoprotein recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans Drosophila sp. (in: flies)

Purification (Commentary)

Purification (Comment) Organism
-
 Drosophila sp. (in: flies)

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system expressed during embryonic development in a tissue- and stage-specific fashion, with elevated expression in a subset of cells within the central nervous system Drosophila sp. (in: flies)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine 36% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R exhibits highest activity toward GalNAcbeta1,4GlcNAc carbohydrate structures at the non-reducing termini of oligosaccharides and glycoprotein glycans. Oligosaccharides are preferred over glycoproteins as acceptors, and no activity toward glycolipid acceptors is detected. Recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R
-
 ?
CMP-N-acetylneuraminate + Galbeta(1-3)-GlcNAcbeta(1-3)Galbeta(1-4)Glc 30% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + NeuNAcalpha(2-6)-Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
CMP-N-acetylneuraminate + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc 79% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-(2-6)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
CMP-N-acetylneuraminate + GalNAcbeta1,4-GlcNAc-R
-
 Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-GalNAcbeta1,4-GlcNAc-R 190% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine ?

Synonyms

Synonyms Comment Organism
SiaT
-
 Drosophila sp. (in: flies)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 activity at 25°C is about 75% of the activity at 100°C Drosophila sp. (in: flies)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Drosophila sp. (in: flies)

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 about 40% of maximal activity at pH 5.0 and at pH 7.5 Drosophila sp. (in: flies)