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Literature summary for 2.4.3.1 extracted from
- Specific stimulation of alpha 2-6 sialyltransferase activity by a novel cytosolic factor from rat colon (1996), Int. J. Biochem. Cell Biol., 28, 1337-1348.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| natural factor from rat colon | stimulation is decreased by CTP, no activation at 0.75 mM CTP | Rattus norvegicus | |
| natural factor from rat colon | partially purified | Rattus norvegicus | |
| natural factor from rat colon | specific, 4fold stimulation by decreasing the Km value for the substrates and increasing Vmax, present in the cytosol, temperature dependent, protease sensitive, independent of metal ions or detergent, unaffceted by monosaccharides | Rattus norvegicus | |
| natural factor from rat colon | may also be present in rat brain and kidney | Rattus norvegicus | |
| Triton X-100 | - |
Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | also decreases the stimulation by the natural cytosolic rat colon factor | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
CMP-N-acetylneuraminate | with stimulation by the natural cytosolic crat colon factor | Rattus norvegicus | |
| 0.23 | - |
asialo-alpha1-acid glycoprotein | with stimulation by the natural cytosolic crat colon factor | Rattus norvegicus | |
| 0.24 | - |
CMP-N-acetylneuraminate | without stimulation by the natural cytosolic crat colon factor | Rattus norvegicus | |
| 0.53 | - |
asialo-alpha1-acid glycoprotein | without stimulation by the natural cytosolic crat colon factor | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley | - |
| Rattus norvegicus | - |
purified enzyme | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| jejunum | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of stimulated and unstimulated enzyme with different substrates | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Rattus norvegicus | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Rattus norvegicus Sprague-Dawley | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
Rattus norvegicus Sprague-Dawley | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | synthetic disaccharides | Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Rattus norvegicus Sprague-Dawley | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Rattus norvegicus Sprague-Dawley | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | synthetic disaccharides | Rattus norvegicus Sprague-Dawley | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Rattus norvegicus |
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