General Stability | Organism |
---|---|
glycerol, 50%, stabilizes during storage | Bos taurus |
high protein concentrations stabilize | Bos taurus |
use of plastic containers instead of glass stabilizes | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions | Bos taurus |
42900 | - |
low molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one | Bos taurus |
44000 | - |
1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions | Bos taurus |
45000 | - |
low molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one | Bos taurus |
53500 | - |
1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions | Bos taurus |
56000 | - |
1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions | Bos taurus |
57900 | - |
high molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one | Bos taurus |
80000 | - |
high molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CDP-hexanolamine-agarose affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colostrum | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | 28 | purified enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, in 50% glycerol, 12 mM cacodylate, pH 5.3, 2 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + asialo-orosomucoid | - |
Bos taurus | CMP + orosomucoid | - |
? | |
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions | Bos taurus |
monomer | 1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions | Bos taurus |
monomer | 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions | Bos taurus |
monomer | 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 5.5 | most stable | Bos taurus |