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Literature summary for 2.4.3.1 extracted from

  • Weinstein, J.; de Souza-e-Silva, U.; Paulson, J.C.
    Sialylation of glycoprotein oligosaccharides N-linked to asparagine. Enzymatic characterization of a Gal beta 1-3(4)GlcNAc alpha 2-3 sialyltransferase and a Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase from rat liver (1982), J. Biol. Chem., 257, 13845-13853.
    View publication on PubMed
Search for more literature for 2.4.3.1

Inhibitors

Inhibitors Comment Organism Structure
CDP Mn2+ restores activity Rattus norvegicus
CTP
-
 Rattus norvegicus
EDTA weak Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Rattus norvegicus
0.0053
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
0.016
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
0.14
-
 asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus
0.21
-
 asialo-transferrin
-
 Rattus norvegicus
1.62
-
 N-acetyllactosamine
-
 Rattus norvegicus
1.67
-
 lacto-N-neotetraose
-
 Rattus norvegicus
129
-
 lactose
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Rattus norvegicus 5794
-
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-agalacto-prothrombin
-
 Rattus norvegicus CMP + agalacto-prothrombin
-
 ?
CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Rattus norvegicus CMP + orosomucoid
-
 ?
CMP-N-acetylneuraminate + asialo-prothrombin
-
 Rattus norvegicus CMP + prothrombin
-
 ?
CMP-N-acetylneuraminate + asialo-transferrin
-
 Rattus norvegicus CMP + transferrin
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to terminal positions on N-linked glycans Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + lacto-N-neotetraose
-
 Rattus norvegicus ?
-
 ?
CMP-N-acetylneuraminate + lactose poor substrate Rattus norvegicus CMP + 6'-sialyllactose
-
 ?
additional information no activity with submaxillary asialo-mucin Rattus norvegicus ?
-
 ?
additional information very low activity with prothrombin and native alpha1-acid glycoprotein, no activity with transferrin, asialo-agalacto-alpha1-acid glycoprotein Rattus norvegicus ?
-
 ?
additional information no activity with antifreeze glycoprotein Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
Galbeta1,4-GlcNAc alpha2,6 sialyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
 30 s, 90% loss of activity Rattus norvegicus