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Literature summary for 2.4.2.64 extracted from

  • Todorov, K.A.; Garcia, G.A.
    Role of aspartate 143 in Escherichia coli tRNA-guanine transglycosylase: alteration of heterocyclic substrate specificity (2006), Biochemistry, 45, 617-625.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.64

Inhibitors

Inhibitors Comment Organism Structure
xanthine for the mutant enzyms D143S and D143T, xanthine is competitive, with respect to guanine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 xanthine 37°C, pH 7.3, mutant enzyme D143N Escherichia coli
0.0114
-
 guanine 37°C, pH 7.3, mutant enzyme D143A Escherichia coli
0.013
-
 xanthine 37°C, pH 7.3, mutant enzyme D143A Escherichia coli
0.0218
-
 guanine 37°C, pH 7.3, mutant enzyme D143N Escherichia coli
0.057
-
 guanine 37°C, pH 7.3, mutant enzyme D143S Escherichia coli
0.105
-
 guanine 37°C, pH 7.3, mutant enzyme D143T Escherichia coli
0.224
-
 xanthine 37°C, pH 7.3, wild-type enzyme Escherichia coli
0.49
-
 hypoxanthine 37°C, pH 7.3, mutant enzyme D143N Escherichia coli
0.8 11 hypoxanthine 37°C, pH 7.3, wild-type enzyme Escherichia coli
1.55
-
 hypoxanthine 37°C, pH 7.3, mutant enzyme D143A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNAqueuine + guanine
-
 Escherichia coli tRNAguanine + queuine
-
 ?
tRNAqueuine + hypoxanthine
-
 Escherichia coli tRNAhypoxanthine + queuine
-
 ?
tRNAqueuine + xanthine
-
 Escherichia coli tRNAxanthine + queuine
-
 ?

Synonyms

Synonyms Comment Organism
TGT
-
 Escherichia coli
tRNA-guanine transglycosylase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000055
-
 xanthine 37°C, pH 7.3, mutant enzyme D143A Escherichia coli
0.0000088
-
 xanthine 37°C, pH 7.3, mutant enzyme D143N Escherichia coli
0.000069
-
 xanthine 37°C, pH 7.3, wild-type enzyme Escherichia coli
0.00012
-
 hypoxanthine 37°C, pH 7.3, mutant enzyme D143N Escherichia coli
0.00019
-
 hypoxanthine 37°C, pH 7.3, mutant enzyme D143A Escherichia coli
0.00297
-
 hypoxanthine 37°C, pH 7.3, wild-type enzyme Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.09
-
 xanthine 37°C, pH 7.3, mutant enzyme D143T Escherichia coli
0.12
-
 xanthine 37°C, pH 7.3, mutant enzyme D143S Escherichia coli