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Literature summary for 2.4.2.6 extracted from
- Ca2+-induced stabilization of the nucleoside 2'-deoxyribosyltransferase from Lactobacillus hilgardii ZJS01 Characteristics and application in nucleosides synthesis (2018), Int. J. Biol. Macromol., 106, 963-968 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | compared with NPTs (EC 2.4.2.5), NDTs present the advantage of catalyzing transglycosylation reactions between purine or pyrimidine bases and nucleosides in one enzyme one-pot mode | Lentilactobacillus hilgardii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ndt, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lentilactobacillus hilgardii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong inhibition | Lentilactobacillus hilgardii |  |
| Mg2+ | strong inhibition | Lentilactobacillus hilgardii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes the enzyme 1t 10-20 mM, Ca2+ markedly improves the thermostability of LhNDT, significantly stimulates the LhNDT activity up to 184% at 10 mM | Lentilactobacillus hilgardii | |
| Co2+ | significantly stimulates the LhNDT activity up to 146% at 10 mM | Lentilactobacillus hilgardii | |
| Mn2+ | significantly stimulates the LhNDT activity up to 127% at 10 mM | Lentilactobacillus hilgardii | |
| additional information | no significant changes are observed with Ba2+ and Cr3+ | Lentilactobacillus hilgardii | |
| Ni2+ | significantly stimulates the LhNDT activity up to 138% at 10 mM | Lentilactobacillus hilgardii | |
| Zn2+ | significantly stimulates the LhNDT activity up to 124% at 10 mM | Lentilactobacillus hilgardii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
recombinant His-tagged enzyme, gel filtration | Lentilactobacillus hilgardii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-ribosyl-base1 + base2 | Lentilactobacillus hilgardii | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
| 2-deoxy-D-ribosyl-base1 + base2 | Lentilactobacillus hilgardii ZJS01 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentilactobacillus hilgardii | A0A286R847 | - |
- |
| Lentilactobacillus hilgardii ZJS01 | A0A286R847 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Lentilactobacillus hilgardii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyuridine + 2,6-diaminopurine | - |
Lentilactobacillus hilgardii | 2,6-diaminopurine-2'-deoxyriboside + uracil | - |
? | |
| 2'-deoxyuridine + 2,6-diaminopurine | - |
Lentilactobacillus hilgardii ZJS01 | 2,6-diaminopurine-2'-deoxyriboside + uracil | - |
? | |
| 2'-deoxyuridine + 5-azacytosine | - |
Lentilactobacillus hilgardii | 5-aza-2'-deoxycytidine + uracil | - |
? | |
| 2'-deoxyuridine + 5-azacytosine | - |
Lentilactobacillus hilgardii ZJS01 | 5-aza-2'-deoxycytidine + uracil | - |
? | |
| 2'-deoxyuridine + 5-fluorocytosine | - |
Lentilactobacillus hilgardii | 5-fluoro-2'-deoxycytidine + uracil | - |
? | |
| 2'-deoxyuridine + 5-fluorocytosine | - |
Lentilactobacillus hilgardii ZJS01 | 5-fluoro-2'-deoxycytidine + uracil | - |
? | |
| 2-deoxy-D-ribosyl-base1 + base2 | - |
Lentilactobacillus hilgardii | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
| 2-deoxy-D-ribosyl-base1 + base2 | - |
Lentilactobacillus hilgardii ZJS01 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
| additional information | NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines | Lentilactobacillus hilgardii | ? | - |
- |
|
| additional information | NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines | Lentilactobacillus hilgardii ZJS01 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21000, recombinant His-tagged enzyme, SDS-PAGE and gel filtration | Lentilactobacillus hilgardii |
| More | LhNDT is a monomer as revealed by gel filtration with or without addition of Ca2+ in elution buffer | Lentilactobacillus hilgardii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GQR93_14380 | locus name | Lentilactobacillus hilgardii |
| LhNDT | - |
Lentilactobacillus hilgardii |
| nucleoside 2'-deoxyribosyltransferase | - |
Lentilactobacillus hilgardii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | assay at | Lentilactobacillus hilgardii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
LhNDT is thermosensitive. Ca2+ markedly improves the thermostability of LhNDT. The enzyme activity decreases sharply when no Ca2+ is added and is completely lost within 6 h at 35°C. Such inactivity is reversed significantly with the addition of Ca2+ at concentrations ranging from 5 mM to 20 mM, and 80 % of the initial activity could be maintained for at least 12 h at a concentration higher than 10 mM. Stability is most significantly enhanced in the presence of 15 mM Ca2+ (the specific concentration of Ca2+ is 0.106 mmol/mg). | Lentilactobacillus hilgardii |
| 30 | 35 | with the addition of 15 mM Ca2+, the thermostability of LhNDT is greatly promoted by 68 and 47 times at 30°C and 35°C, respectively. LhNDT retains approx. 40% activity after 1 h at 35°C | Lentilactobacillus hilgardii |
| 60 | - |
half-life of the free LrNDT is 17.9 min | Lentilactobacillus hilgardii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Lentilactobacillus hilgardii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.4 | purified recombinant LhNDT retains more than 90% of its original activity between after 1 h | Lentilactobacillus hilgardii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | NDTs are classified into two classes depending on their substrate specificity: NDT type I (PDT), specific for purine exchange, and NDT type II (NDT), which catalyzes the transfer between purines and/or pyrimidines. LhNDT is classified as a NDT type II | Lentilactobacillus hilgardii |
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