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Literature summary for 2.4.2.6 extracted from

  • Crespo, N.; Sanchez-Murcia, P.A.; Gago, F.; Cejudo-Sanches, J.; Galmes, M.A.; Fernandez-Lucas, J.; Mancheno, J.M.
    2'-Deoxyribosyltransferase from Leishmania mexicana, an efficient biocatalyst for one-pot, one-step synthesis of nucleosides from poorly soluble purine bases (2017), Appl. Microbiol. Biotechnol., 101, 7187-7200 .
    View publication on PubMed
Search for more literature for 2.4.2.6

Application

Application Comment Organism
synthesis potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine Leishmania mexicana

Cloned(Commentary)

Cloned (Comment) Organism
gene pdt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana U1103
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana MHOM
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana 2001
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana GT
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania mexicana E9AWJ0
-
-
Leishmania mexicana 2001 E9AWJ0
-
-
Leishmania mexicana GT E9AWJ0
-
-
Leishmania mexicana MHOM E9AWJ0
-
-
Leishmania mexicana U1103 E9AWJ0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana 2',3'-dideoxyguanosine + adenine
-
 ?
2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana U1103 2',3'-dideoxyguanosine + adenine
-
 ?
2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana MHOM 2',3'-dideoxyguanosine + adenine
-
 ?
2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana 2001 2',3'-dideoxyguanosine + adenine
-
 ?
2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana GT 2',3'-dideoxyguanosine + adenine
-
 ?
2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana 2',3'-dideoxyinosine + adenine
-
 ?
2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana U1103 2',3'-dideoxyinosine + adenine
-
 ?
2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana MHOM 2',3'-dideoxyinosine + adenine
-
 ?
2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana 2001 2',3'-dideoxyinosine + adenine
-
 ?
2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana GT 2',3'-dideoxyinosine + adenine
-
 ?
2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana U1103 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana MHOM 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana 2001 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana GT 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2',3'-dideoxyinosine + guanine low activity Leishmania mexicana 2',3'-dideoxyguanosine + hypoxanthine
-
 ?
2'-deoxyadenosine + guanine
-
 Leishmania mexicana 2'-deoxyguanosine + adenine
-
 ?
2'-deoxyguanosine + adenine
-
 Leishmania mexicana 2'-deoxyadenosine + guanine
-
 ?
2'-deoxyinosine + adenine
-
 Leishmania mexicana 2'-deoxyadenosine + hypoxanthine
-
 ?
2'-deoxyinosine + adenine
-
 Leishmania mexicana U1103 2'-deoxyadenosine + hypoxanthine
-
 ?
2'-deoxyinosine + adenine
-
 Leishmania mexicana MHOM 2'-deoxyadenosine + hypoxanthine
-
 ?
2'-deoxyinosine + adenine
-
 Leishmania mexicana 2001 2'-deoxyadenosine + hypoxanthine
-
 ?
2'-deoxyinosine + adenine
-
 Leishmania mexicana GT 2'-deoxyadenosine + hypoxanthine
-
 ?
2'-deoxyinosine + guanine
-
 Leishmania mexicana 2'-deoxyguanosine + hypoxanthine
-
 ?
2'-fluoro-2'-deoxyadenosine + guanine
-
 Leishmania mexicana 2'-fluoro-2'-deoxyguanosine + adenine
-
 ?
2'-fluoro-2'-deoxyinosine + adenine high activity Leishmania mexicana 2'-fluoro-2'-deoxyadenosine + hypoxanthine
-
 ?
2'-fluoro-2'-deoxyinosine + guanine high activity Leishmania mexicana 2'-fluoro-2'-deoxyguanosine + hypoxanthine
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana U1103 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana MHOM 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana 2001 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana GT 2-deoxy-D-ribosyl-base2 + base1
-
 ?
9-beta-D-arabinofuranosyl adenine + guanine low activity Leishmania mexicana ?
-
 ?
9-beta-D-arabinofuranosyl adenine + hypoxanthine low activity Leishmania mexicana ?
-
 ?
9-beta-D-arabinofuranosyl guanine + adenine
-
 Leishmania mexicana ?
-
 ?
9-beta-D-arabinofuranosyl guanine + hypoxanthine low activity Leishmania mexicana ?
-
 ?
9-beta-D-arabinofuranosyl hypoxanthine + adenine
-
 Leishmania mexicana ?
-
 ?
9-beta-D-arabinofuranosyl hypoxanthine + guanine low activity Leishmania mexicana ?
-
 ?

Synonyms

Synonyms Comment Organism
2'-deoxyribosyltransferase
-
 Leishmania mexicana
LmPDT
-
 Leishmania mexicana
PDT
-
 Leishmania mexicana
type I NDT
-
 Leishmania mexicana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
 Leishmania mexicana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 over 50% of maximal activity within this range Leishmania mexicana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant enzyme LmPDT remains stable during 36 h at different pH values Leishmania mexicana
95
-
 purified recombinant enzyme LmPDT, 5 min, inactivation Leishmania mexicana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Leishmania mexicana

pH Range

pH Minimum pH Maximum Comment Organism
4 10 over 6% of maximal activity within this range Leishmania mexicana

General Information

General Information Comment Organism
physiological function N-deoxyribosyltransferases catalyze the direct transfer of the 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor through a one-step reaction Leishmania mexicana