BRENDA - Enzyme Database
show all sequences of 2.4.2.59

From suicide enzyme to catalyst the iron-dependent sulfide transfer in Methanococcus jannaschii thiamin thiazole biosynthesis

Eser, B.; Zhang, X.; Chanani, P.; Begley, T.; Ealick, S.; J. Am. Chem. Soc. 138, 3639-3642 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
may substitute for iron
Methanocaldococcus jannaschii
Co2+
may substitute for iron
Methanocaldococcus jannaschii
Fe2+
highest levels of activity in presence of FE2+ or Fe3+, iron is involved in sulfur transfer
Methanocaldococcus jannaschii
Fe3+
highest levels of activity in presence of FE2+ or Fe3+, iron is involved in sulfur transfer
Methanocaldococcus jannaschii
Mg2+
may substitute for iron
Methanocaldococcus jannaschii
Mn2+
may substitute for iron
Methanocaldococcus jannaschii
additional information
Cu+ or Cu2+ do not support activity
Methanocaldococcus jannaschii
Ni2+
may substitute for iron
Methanocaldococcus jannaschii
Zn2+
may substitute for iron
Methanocaldococcus jannaschii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q58018
-
-
Methanocaldococcus jannaschii DSM 2661
Q58018
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + glycine + sulfide
-
745168
Methanocaldococcus jannaschii
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
-
-
?
NAD+ + glycine + sulfide
-
745168
Methanocaldococcus jannaschii DSM 2661
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
may substitute for iron
Methanocaldococcus jannaschii
Co2+
may substitute for iron
Methanocaldococcus jannaschii
Fe2+
highest levels of activity in presence of FE2+ or Fe3+, iron is involved in sulfur transfer
Methanocaldococcus jannaschii
Fe3+
highest levels of activity in presence of FE2+ or Fe3+, iron is involved in sulfur transfer
Methanocaldococcus jannaschii
Mg2+
may substitute for iron
Methanocaldococcus jannaschii
Mn2+
may substitute for iron
Methanocaldococcus jannaschii
additional information
Cu+ or Cu2+ do not support activity
Methanocaldococcus jannaschii
Ni2+
may substitute for iron
Methanocaldococcus jannaschii
Zn2+
may substitute for iron
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + glycine + sulfide
-
745168
Methanocaldococcus jannaschii
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
-
-
?
NAD+ + glycine + sulfide
-
745168
Methanocaldococcus jannaschii DSM 2661
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H2O
-
-
-
?
Other publictions for EC 2.4.2.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745168
Eser
From suicide enzyme to cataly ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Am. Chem. Soc.
138
3639-3642
2016
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744796
Hoppenau
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Verticillium dahliae VdTHI4, ...
Verticillium dahliae, Verticillium dahliae JR2
Environ. Exp. Bot.
108
14-22
2014
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