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Literature summary for 2.4.2.36 extracted from
- An Entamoeba histolytica ADP-ribosyl transferase from the diphtheria toxin family modifies the bacterial elongation factor Tu (2016), Mol. Biochem. Parasitol., 207, 68-74 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C43 (DE3) cells | Entamoeba histolytica |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 12% (w/v) polyethylene glycol 6000, 0.1 M MES pH 5.5, 0.1 M ammonium acetate | Entamoeba histolytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E571A | catalytically inactive | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + diphthamide-[Escherichia coli elongation factor Tu] | Entamoeba histolytica | - |
nicotinamide + N-(ADP-D-ribosyl)diphthamide-[Escherichia coli elongation factor Tu] | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | C4LWB1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + diphthamide-[Escherichia coli elongation factor Tu] | - |
Entamoeba histolytica | nicotinamide + N-(ADP-D-ribosyl)diphthamide-[Escherichia coli elongation factor Tu] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Entamoeba histolytica |
| ? | x * 43654, calculated from amino acid sequence | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EHI 155600 | locus name | Entamoeba histolytica |
| EhToxin-l | - |
Entamoeba histolytica |
| EhToxin-like | - |
Entamoeba histolytica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is an important virulence factor from Aeromonas pathogenic species | Entamoeba histolytica |
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Release 2023.1 (January 2023)
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