BRENDA - Enzyme Database show
show all sequences of 2.4.2.29

Studies with a homogeneous enzyme from rabbit erythrocytes catalyzing the insertion of guanine into tRNA

Howes, N.K.; Farkas, W.R.; J. Biol. Chem. 253, 9082-9087 (1978)

Data extracted from this reference:

General Stability
General Stability
Organism
linear KCl gradient to elute the enzyme from phosphocellulose results in complete loss of activity
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000033
-
tRNAAsp
yeast tRNAAsp
Oryctolagus cuniculus
0.00015
-
guanine
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
60000
-
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
104000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Oryctolagus cuniculus
post-translational modification of tRNA
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
erythrocyte
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
-80°C, 10% glycerol, 25% loss of activity per month
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
guanine + tRNAguanine
yeast tRNAAsp
644918
Oryctolagus cuniculus
tRNAguanine + guanine
-
-
-
?
additional information
post-translational modification of tRNA
644918
Oryctolagus cuniculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
General Stability (protein specific)
General Stability
Organism
linear KCl gradient to elute the enzyme from phosphocellulose results in complete loss of activity
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000033
-
tRNAAsp
yeast tRNAAsp
Oryctolagus cuniculus
0.00015
-
guanine
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
60000
-
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
104000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Oryctolagus cuniculus
post-translational modification of tRNA
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
erythrocyte
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 10% glycerol, 25% loss of activity per month
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
guanine + tRNAguanine
yeast tRNAAsp
644918
Oryctolagus cuniculus
tRNAguanine + guanine
-
-
-
?
additional information
post-translational modification of tRNA
644918
Oryctolagus cuniculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 60000 + 1 * 43000, denaturing PAGE in presence of urea
Oryctolagus cuniculus
Other publictions for EC 2.4.2.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735961
Barandun
Replacement of water molecules ...
Zymomonas mobilis
Chemistry
21
126-135
2015
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8
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2
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1
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2
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8
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8
8
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2
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2
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-
736521
Sawhney
Identification of Plasmodium f ...
Plasmodium falciparum
J. Biomol. Struct. Dyn.
33
2404-2420
2015
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-
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1
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1
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3
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1
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1
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1
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1
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728740
Nomura
Purification and comparison of ...
Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834
Protein Expr. Purif.
88
13-19
2013
-
-
1
-
-
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-
1
-
1
3
-
-
4
-
-
1
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-
-
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-
1
1
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1
1
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1
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1
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1
3
-
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1
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-
-
1
1
-
-
-
1
1
-
-
-
1
1
-
-
-
735359
Barandun
High-affinity inhibitors of Zy ...
Zymomonas mobilis
Acta Crystallogr. Sect. D
69
1798-1807
2013
-
-
-
1
-
-
-
-
-
-
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1
-
2
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-
-
-
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-
-
1
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1
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1
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-
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-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
722058
Barandun
From lin-benzoguanines to lin- ...
Zymomonas mobilis
Chemistry
18
9246-9257
2012
-
-
-
-
-
-
14
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
14
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
721496
Eric Thomas
Differential heterocyclic subs ...
Escherichia coli, Homo sapiens
Biochem. Biophys. Res. Commun.
410
34-39
2011
-
-
-
-
4
-
4
6
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
2
-
-
6
2
-
-
-
10
-
-
-
-
-
-
-
4
-
-
4
10
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
6
2
-
-
-
-
-
-
-
6
6
706730
Chen
Characterization of the human ...
Homo sapiens
RNA
16
958-968
2010
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702263
Garcia
Identification of the rate-det ...
Escherichia coli
Biochemistry
48
11243-11251
2009
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
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-
-
3
-
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-
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2
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1
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2
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1
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-
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3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
703202
Ritschel
Crystal structure analysis and ...
Zymomonas mobilis
ChemBioChem
10
716-727
2009
-
-
1
1
-
-
10
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
-
-
1
-
1
-
-
-
10
10
-
-
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-
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-
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703213
Kohler
High-affinity inhibitors of tR ...
Zymomonas mobilis
Chemistry
15
10809-10817
2009
-
-
-
1
-
-
30
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
1
-
-
-
30
11
-
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703226
Ritschel
How to replace the residual so ...
Zymomonas mobilis
ChemMedChem
4
2012-2023
2009
-
-
-
1
1
-
4
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
4
-
4
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
705164
Ritschel
An integrative approach combin ...
Zymomonas mobilis
J. Mol. Biol.
393
833-847
2009
-
-
1
1
2
-
5
3
-
1
3
-
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
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-
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-
-
1
-
1
2
-
-
5
-
3
-
1
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
681439
Stengl
Crystal structures of tRNA-gua ...
Zymomonas mobilis
J. Mol. Biol.
370
492-511
2007
-
1
-
1
-
-
18
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
1
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-
-
-
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-
-
17
-
-
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1
-
-
2
-
-
-
18
17
-
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-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684430
Hoertner
Potent inhibitors of tRNA-guan ...
Shigella flexneri
Angew. Chem.
46
8266-8269
2007
-
-
-
-
-
-
12
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
12
-
-
-
-
-
-
-
-
-
-
12
12
-
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-
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
688390
Tidten
Glutamate versus glutamine exc ...
Zymomonas mobilis
J. Mol. Biol.
374
764-776
2007
-
-
1
1
1
-
-
8
-
-
-
-
-
3
-
-
-
-
-
-
-
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2
-
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8
-
-
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-
1
-
1
1
-
-
-
-
8
-
-
-
-
-
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-
-
-
-
-
-
2
-
-
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-
8
-
-
-
-
-
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-
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-
689250
Hurt
Site-specific modification of ...
Escherichia coli
Nucleic Acids Res.
35
4905-4913
2007
-
-
-
-
-
-
-
3
-
-
-
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6
-
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3
-
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3
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3
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-
-
3
-
-
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3
-
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-
678191
Todorov
Role of aspartate 143 in Esche ...
Escherichia coli
Biochemistry
45
617-625
2006
-
-
-
-
-
-
1
10
-
-
-
-
-
2
-
-
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-
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-
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3
-
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6
-
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-
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2
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-
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1
2
10
-
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-
-
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-
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-
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
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-
680057
Meyer
-
Synthesis, biological evaluati ...
Zymomonas mobilis
Helv. Chim. Acta
89
573-597
2006
-
-
-
-
-
-
13
-
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-
1
-
-
-
-
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-
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-
-
-
-
-
-
-
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-
-
13
-
-
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-
-
-
-
-
-
-
13
13
-
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-
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-
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-
-
658289
Todorov
The role of aspartic acid 143 ...
Escherichia coli
Biophys. J.
89
1965-1977
2005
-
-
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4
-
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1
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5
-
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2
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4
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1
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2
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-
679204
Stengl
Mechanism and substrate specif ...
Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Zymomonas mobilis
Chembiochem
6
1926-1939
2005
-
-
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1
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4
-
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1
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658379
Ishiwata
Elevated expression level of 6 ...
Homo sapiens
Cancer Lett.
212
113-119
2004
-
-
-
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1
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1
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1
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2
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1
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1
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1
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2
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1
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-
-
-
-
-
-
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-
644928
Brenk
Virtual screening for submicro ...
Shigella flexneri
J. Med. Chem.
46
1133-1143
2003
-
-
-
-
-
-
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644939
Goodenough-Lashua
tRNA-guanine transglycosylase ...
Escherichia coli, Shigella flexneri
Bioorg. Chem.
31
331-344
2003
-
1
-
-
-
-
1
-
-
-
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2
-
5
-
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-
-
-
-
-
-
-
-
-
644940
Kittendorf
An essential role for aspartat ...
Escherichia coli, Zymomonas mobilis
J. Biol. Chem.
278
42369-42376
2003
-
-
-
1
6
-
-
6
-
-
1
-
-
5
-
-
-
1
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658446
Brenk
Flexible adaptations in the st ...
Zymomonas mobilis
ChemBioChem
4
1066-1077
2003
-
-
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1
1
-
4
4
-
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-
1
-
4
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644931
Nonekowski
The Escherichia coli tRNA-guan ...
Escherichia coli, Pyrococcus horikoshii
J. Biol. Chem.
277
7178-7182
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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-
-
-
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-
-
-
644929
Ishiwata
Increased expression of queuos ...
Homo sapiens
J. Biochem.
129
13-17
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
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-
644933
Kittendorf
tRNA-guanine transglycosylase ...
Escherichia coli
Biochemistry
40
14123-14133
2001
-
-
1
-
4
-
-
8
-
-
-
-
-
3
-
-
1
-
-
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-
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-
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-
8
-
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-
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-
644937
Nonekowski
tRNA recognition by tRNA-guani ...
Escherichia coli
RNA
7
1432-1441
2001
-
-
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-
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-
-
1
-
3
-
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-
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-
-
-
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-
644938
Kung
tRNA-guanine transglycosylase ...
Escherichia coli
RNA
6
233-244
2000
-
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-
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-
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-
-
-
-
-
-
6
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
644926
Kung
tRNA-guanine transglycosylase ...
Escherichia coli
FEBS Lett.
431
427-432
1998
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644927
Romier
Slight sequence variations of ...
Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Synechocystis sp., Thermotoga maritima
FEBS Lett.
416
93-98
1997
-
-
-
-
-
-
-
-
-
-
8
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644930
Romier
Crystal structure of tRNA-guan ...
Zymomonas mobilis
EMBO J.
15
2850-2857
1996
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644936
Deshpande
Cloning and characterization o ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
326
1-7
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644932
Curnow
tRNA-guanine transglycosylase ...
Escherichia coli
J. Biol. Chem.
270
17264-17267
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644935
Hoops
Mechanism-based inactivation o ...
Escherichia coli
Biochemistry
34
15539-15544
1995
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
644934
Reuter
Serine 90 is required for enzy ...
Escherichia coli
Biochemistry
33
7041-7046
1994
-
-
1
-
3
-
-
4
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644920
Garcia
tRNA-guanine transglycosylase ...
Escherichia coli
J. Mol. Biol.
231
489-497
1993
-
-
1
-
-
-
2
-
-
-
-
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644919
Gunduz
-
Transfer -RNA guanine transgly ...
Salmonella enterica subsp. enterica serovar Typhimurium
Period. Biol.
90
321-326
1988
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644922
Haumont
Enzymatic formation of queuosi ...
Xenopus laevis
Eur. J. Biochem.
168
219-225
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644921
Farkas
Substrate and inhibitor specif ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
781
64-75
1984
-
-
-
-
-
-
20
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
20
7
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644924
Carbon
Site-directed in vitro replace ...
Xenopus laevis
EMBO J.
2
1093-1097
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644923
Walden
Purification and properties of ...
Triticum aestivum
J. Biol. Chem.
257
13218-13222
1982
-
-
-
-
-
-
8
1
-
9
2
-
-
1
-
-
1
-
-
1
-
-
3
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
8
1
1
-
9
2
-
-
-
-
1
-
1
-
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
644925
Shindo-Okada
Transfer ribonucleic acid guan ...
Rattus norvegicus
Biochemistry
19
395-400
1980
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
2
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
1
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644917
Okada
Novel mechanism of post-transc ...
Escherichia coli
J. Biol. Chem.
254
3067-3073
1979
-
-
-
-
-
-
-
2
-
-
-
2
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644918
Howes
Studies with a homogeneous enz ...
Oryctolagus cuniculus
J. Biol. Chem.
253
9082-9087
1978
-
-
-
-
-
1
-
2
1
-
3
1
-
1
-
-
1
-
-
1
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
-
3
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-