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Literature summary for 2.4.2.18 extracted from
- Prediction of quaternary structure by analysis of hot spot residues in protein-protein interfaces the case of anthranilate phosphoribosyltransferases (2019), Proteins, 87, 815-825 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells | Staphylococcus aureus |
| expressed in Escherichia coli M15 cells | Staphylococcus haemolyticus |
| expressed in Escherichia coli M15 cells | Methanococcus voltae |
| expressed in Escherichia coli M15 cells | Saccharolobus solfataricus |
| expressed in Escherichia coli M15 cells | Pelodictyon luteolum |
| expressed in Escherichia coli M15 cells | Petrotoga mobilis |
| expressed in Escherichia coli M15 cells | Acetomicrobium mobile |
| expressed in Escherichia coli M15 cells | Fructobacillus fructosus |
| expressed in Escherichia coli M15 cells | Methanocella conradii |
| expressed in Escherichia coli M15 cells | Coprococcus eutactus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Petrotoga mobilis |  |
| 0.0024 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Methanocella conradii | |
| 0.019 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Acetomicrobium mobile | |
| 0.02 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Methanococcus voltae | |
| 0.032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Saccharolobus solfataricus | |
| 0.085 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Coprococcus eutactus | |
| 0.086 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Pelodictyon luteolum | |
| 0.116 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Staphylococcus haemolyticus | |
| 0.15 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Staphylococcus aureus | |
| 0.371 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Fructobacillus fructosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, 0.05 mM used in assay conditions | Saccharolobus solfataricus | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Staphylococcus aureus | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Staphylococcus haemolyticus | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Methanococcus voltae | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Pelodictyon luteolum | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Petrotoga mobilis | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Acetomicrobium mobile | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Fructobacillus fructosus | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Methanocella conradii | |
| Mg2+ | dependent on, 2 mM used in assay conditions | Coprococcus eutactus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
calculated from amino acid sequence | Coprococcus eutactus |
| 78200 | - |
calculated from amino acid sequence | Methanocella conradii |
| 78700 | - |
calculated from amino acid sequence | Petrotoga mobilis |
| 79200 | - |
calculated from amino acid sequence | Saccharolobus solfataricus |
| 83700 | - |
calculated from amino acid sequence | Methanococcus voltae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Staphylococcus aureus | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Staphylococcus haemolyticus | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Methanococcus voltae | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Pelodictyon luteolum | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Petrotoga mobilis | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Acetomicrobium mobile | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Fructobacillus fructosus | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Methanocella conradii | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Coprococcus eutactus | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus P2 | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Staphylococcus aureus USA300 | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Pelodictyon luteolum DSM270 | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Staphylococcus haemolyticus JCSC1435 | - |
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetomicrobium mobile | - |
- |
- |
| Coprococcus eutactus | - |
- |
- |
| Fructobacillus fructosus | - |
- |
- |
| Methanocella conradii | - |
- |
- |
| Methanococcus voltae | - |
- |
- |
| Pelodictyon luteolum | - |
- |
- |
| Pelodictyon luteolum DSM270 | - |
- |
- |
| Petrotoga mobilis | - |
- |
- |
| Saccharolobus solfataricus | P50384 | - |
- |
| Saccharolobus solfataricus P2 | P50384 | - |
- |
| Staphylococcus aureus | - |
- |
- |
| Staphylococcus aureus USA300 | - |
- |
- |
| Staphylococcus haemolyticus | - |
- |
- |
| Staphylococcus haemolyticus JCSC1435 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mono Q column chromatography | Methanococcus voltae |
| Mono Q column chromatography | Saccharolobus solfataricus |
| Mono Q column chromatography | Petrotoga mobilis |
| Mono Q column chromatography | Acetomicrobium mobile |
| Mono Q column chromatography | Fructobacillus fructosus |
| Mono Q column chromatography | Coprococcus eutactus |
| MonoQ column chromatography | Staphylococcus aureus |
| MonoQ column chromatography | Staphylococcus haemolyticus |
| MonoQ column chromatography | Pelodictyon luteolum |
| MonoQ column chromatography | Methanocella conradii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Staphylococcus aureus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Staphylococcus haemolyticus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Methanococcus voltae | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Pelodictyon luteolum | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Petrotoga mobilis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Acetomicrobium mobile | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Fructobacillus fructosus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Methanocella conradii | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Coprococcus eutactus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus P2 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Staphylococcus aureus USA300 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Pelodictyon luteolum DSM270 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Staphylococcus haemolyticus JCSC1435 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 37500, calculated from amino acid sequence | Coprococcus eutactus |
| homodimer | 2 * 41900, calculated from amino acid sequence | Methanococcus voltae |
| monomer | 1 * 38500, calculated from amino acid sequence | Staphylococcus aureus |
| monomer | 1 * 39400, calculated from amino acid sequence | Staphylococcus haemolyticus |
| monomer | 1 * 39800, calculated from amino acid sequence | Pelodictyon luteolum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AnPRT | - |
Staphylococcus aureus |
| AnPRT | - |
Staphylococcus haemolyticus |
| AnPRT | - |
Methanococcus voltae |
| AnPRT | - |
Saccharolobus solfataricus |
| AnPRT | - |
Pelodictyon luteolum |
| AnPRT | - |
Petrotoga mobilis |
| AnPRT | - |
Acetomicrobium mobile |
| AnPRT | - |
Fructobacillus fructosus |
| AnPRT | - |
Methanocella conradii |
| AnPRT | - |
Coprococcus eutactus |
| TrpD | - |
Staphylococcus aureus |
| TrpD | - |
Staphylococcus haemolyticus |
| TrpD | - |
Methanococcus voltae |
| TrpD | - |
Saccharolobus solfataricus |
| TrpD | - |
Pelodictyon luteolum |
| TrpD | - |
Petrotoga mobilis |
| TrpD | - |
Acetomicrobium mobile |
| TrpD | - |
Fructobacillus fructosus |
| TrpD | - |
Methanocella conradii |
| TrpD | - |
Coprococcus eutactus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44.7 | - |
melting temperature | Staphylococcus aureus |
| 44.8 | - |
melting temperature | Staphylococcus haemolyticus |
| 49.8 | - |
melting temperature | Pelodictyon luteolum |
| 55.3 | - |
melting temperature | Coprococcus eutactus |
| 56.2 | - |
melting temperature | Petrotoga mobilis |
| 62.1 | - |
melting temperature | Methanococcus voltae |
| 68.3 | - |
melting temperature | Methanocella conradii |
| 91.1 | - |
melting temperature | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00016 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Acetomicrobium mobile | |
| 0.00067 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Methanococcus voltae | |
| 0.13 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Methanocella conradii | |
| 0.18 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Saccharolobus solfataricus | |
| 0.25 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Petrotoga mobilis | |
| 0.27 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Coprococcus eutactus | |
| 0.28 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Pelodictyon luteolum | |
| 0.42 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Staphylococcus haemolyticus | |
| 0.43 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Staphylococcus aureus | |
| 0.44 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.5 and 25°C | Fructobacillus fructosus | |
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