Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
C46A | the mutant shows wild type activity | Bombyx mori |
N183/341Q | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
N183D | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
N183D/N341D | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
N183Q | the mutant shows wild type activity | Bombyx mori |
N341D | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
N341Q | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
R359A | inactive | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Bombyx mori |
120000 | - |
gel filtration | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine | Bombyx mori | - |
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | A0A060PLD2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Asn-183 and Asn-341 are the consensus sites for N-glycosylation | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Bombyx mori | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 66000, SDS-PAGE | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
alpha1,6-fucosyltransferase | - |
Bombyx mori |
alpha1,6FucT | - |
Bombyx mori |