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Literature summary for 2.4.1.5 extracted from

  • Lan, W.; Chen, S.
    Chemical kinetics, thermodynamics and inactivation kinetics of dextransucrase activity by ultrasound treatment (2020), React. Kinet. Mech. Catal., 129, 843-864 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information ultrasound treatment significantly increases dextransucrase activity. The optimal ultrasound conditions are an ultrasound intensity of 1.8 W/cm3 and ultrasound time of 3 min, resulting in the dextransucrase activity increasing by 27.6% Leuconostoc mesenteroides

Application

Application Comment Organism
food industry important enzyme in food industry. Ultrasound is a tool for increasing the activity, thermal stability and rate of catalysis of dextransucrase and supplies a potential method for expanding the application of dextransucrase Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.99
-
sucrose 25°C, pH 5.4, ultrasound-treated enzyme Leuconostoc mesenteroides
12.29
-
sucrose 25°C, pH 5.4, untreated enzyme Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides CICC-21724
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
Leuconostoc mesenteroides D-fructose + dextran
-
?
sucrose
-
Leuconostoc mesenteroides CICC-21724 D-fructose + dextran
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the percentage of thermal stability in ultrasound-treated dextransucrase is increased, while the rate of deactivation is decreased. The degree of thermal inactivation is inversely proportional to the dextransucrase concentration Leuconostoc mesenteroides