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Literature summary for 2.4.1.346 extracted from

  • Batt, S.M.; Jabeen, T.; Mishra, A.K.; Veerapen, N.; Krumbach, K.; Eggeling, L.; Besra, G.S.; Fuetterer, K.
    Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB (2010), J. Biol. Chem., 285, 37741-37752.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.346

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Corynebacterium glutamicum
expression in Escherichia coli Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment) Organism
PEG 3350, pH 5.5 Corynebacterium glutamicum
structure of mannosyltransferase PimB in complex with nucleotide to a resolution of 2.0 A. Both the nucleotide conformation as well as the structural framework of the active site display flexibility Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
D13A 1.9% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
D13A 1.9% of wild-type activity Corynebacterium glutamicum
D13N 1.5% of wild-type activity Corynebacterium glutamicum
D13N 1.5% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
D13Y 0.3% of wild-type activity Corynebacterium glutamicum
D13Y 0.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
E290D 2.9% of wild-type activity Corynebacterium glutamicum
E290D 2.9% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
E290N 1.1% of wild-type activity Corynebacterium glutamicum
E290N 1.1% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
E290Q 4.6% of wild-type activity Corynebacterium glutamicum
E290Q 4.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
G123P 86.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
G123P 86.6% of wild-type activity Corynebacterium glutamicum
G20W 3.2% of wild-type activity Corynebacterium glutamicum
G20W 3.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
G291S 5.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
G291S 5.3% of wild-type activity Corynebacterium glutamicum
H118S 0.7% of wild-type activity Corynebacterium glutamicum
H118S 0.7% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
H120S 81.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
H120S 81.2% of wild-type activity Corynebacterium glutamicum
I21A 2.0% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
I21A 2.0% of wild-type activity Corynebacterium glutamicum
I21S 2.4% of wild-type activity Corynebacterium glutamicum
I21S 2.4% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
K211Q 3.5% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
K211Q 3.5% of wild-type activity Corynebacterium glutamicum
N12A 31.2% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
N12A 31.2% of wild-type activity Corynebacterium glutamicum
Q22A 14.3% of wild-type activity Corynebacterium glutamicum
Q22A 14.3% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
R206S 5.6% of wild-type activity Corynebacterium glutamicum
R206S 5.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
R210S 8.7% of wild-type activity Corynebacterium glutamicum
R210S 8.7% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
S205G 119.6% of wild type activity (blank has 0.5% activity) Corynebacterium glutamicum
S205G 119.6% of wild-type activity Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum Q8NNK8
-
-
Corynebacterium glutamicum DSM 20300 Q8NNK8
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannopyranose + phosphatidyl-myo-inositol attaches mannosyl selectively to the 6-OH of the inositol moiety Corynebacterium glutamicum GDP + ?
-
 ?
GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
GDP-alpha-D-mannose + 2-O-(6-O-acyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum DSM 20300 GDP + 2-O-(6-O-acyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
GDP-alpha-D-mannose + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum GDP + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
GDP-alpha-D-mannose + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 Corynebacterium glutamicum DSM 20300 GDP + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
 ?
additional information PimB attaches the mannosyl moiety selectively to the 6-OH of the inositol moiety of phosphatidylinositol Corynebacterium glutamicum ?
-
 ?
additional information PimB attaches the mannosyl moiety selectively to the 6-OH of the inositol moiety of phosphatidylinositol Corynebacterium glutamicum DSM 20300 ?
-
 ?

Synonyms

Synonyms Comment Organism
mannosyltransferase PimB'
-
 Corynebacterium glutamicum
PimB
-
 Corynebacterium glutamicum