Literature summary for 2.4.1.333 extracted from

Kobayashi, K.; Nakajima, M.; Aramasa, H.; Kimura, S.; Iwata, T.; Nakai, H.; Taguchi, H.
Large-scale preparation of beta-1,2-glucan using quite a small amount of sophorose (2019), Biosci. Biotechnol. Biochem., 83, 1867-1874 .

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Application

Application	Comment	Organism
synthesis	synthesis of beta-1,2-glucan with sophorose as an acceptor through three synthesis steps using sucrose phosphorylase and 1,2-beta-oligoglucan phosphorylase. The first step is performed in 200 microl reaction solution containing 5 mM sophorose and 1.0 M sucrose. beta-1,2-Glucan in a part of the resultant solution is hydrolyzed to beta-1,2-glucooligosaccharides by beta-1,2-glucanase. The second synthesis is performed in 25 times the volume for the first synthesis. The hydrolysate solution (1% volume of the reaction solution) is used as an acceptor. After treatment with the beta-1,2-glucanase again, the third synthesis is performed in 200 times the volume of the second synthesis (1 l). More than 140 g of beta-1,2-glucan is synthesized using approximately 20 microg of sophorose as the starting acceptor material	Listeria innocua Clip11262

Organism

Organism	UniProt	Comment	Textmining
Listeria innocua Clip11262	Q92AT0	-	-
Listeria innocua Clip11262 ATCC BAA-680	Q92AT0	-	-

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Release 2023.1 (January 2023)