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Information on EC 2.4.1.333 - 1,2-beta-oligoglucan phosphorylase
for references in articles please use BRENDA:EC2.4.1.333
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.333 1,2-beta-oligoglucan phosphorylase
IUBMB Comments
The enzyme has been isolated from the bacterium Listeria innocua. It catalyses the reversible phosphorolysis of β-(1→2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 D-glucose units. In the synthetic reaction starting from sophorose and α-D-glucose 1-phosphate the average polymerisation degree is 39.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
showSynonyms
lin1839, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,2-beta-D-oligoglucan phosphorylase
-
-
-
-
Cphy_0694
EiSOGP
lin1839
SOGP
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[(1->2)-beta-D-glucosyl]n + phosphate = [(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
1,2-beta-D-glucan:phosphate alpha-D-glucosyltransferase
The enzyme has been isolated from the bacterium Listeria innocua. It catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 D-glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucopyranose 1-phosphate
Substrates: -
Products: -
Products: -
ir
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
sophorose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
ir
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
O-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucopyranosyl]2-(1->2)-3-deoxy-D-glucofuranose + phosphate
sophorose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
?
O-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucopyranosyl]2-(1->2)-3-deoxy-D-glucofuranose + phosphate
Substrates: -
Products: -
Products: -
?
O-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucopyranosyl]2-(1->2)-3-deoxy-D-glucofuranose + phosphate
Substrates: -
Products: -
Products: -
?
O-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucopyranosyl]2-(1->2)-3-deoxy-D-glucofuranose + phosphate
Substrates: -
Products: -
Products: -
?
O-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucopyranosyl]2-(1->2)-3-deoxy-D-glucofuranose + phosphate
Listeria innocua Clip11262 ATCC BAA-680
Substrates: -
Products: -
Products: -
?
sophorose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
sophorose + alpha-D-glucose 1-phosphate
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
sophorose + alpha-D-glucose 1-phosphate
sophorotriose + phosphate
Substrates: -
Products: -
Products: -
?
sophorose + alpha-D-glucose 1-phosphate
sophorotriose + phosphate
Substrates: -
Products: -
Products: -
?
sophorose + alpha-D-glucose 1-phosphate
sophorotriose + phosphate
Substrates: -
Products: -
Products: -
?
sophorose + alpha-D-glucose 1-phosphate
sophorotriose + phosphate
Listeria innocua Clip11262 ATCC BAA-680
Substrates: -
Products: -
Products: -
?
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the average product shows a polymerisation degree of 39 glucose units
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the average product shows a polymerisation degree of 39 glucose units
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the enzyme catalyses the reversible phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
additional information
?
-
Substrates: the 3'-hydroxy group of sophorose is important for substrate recognition. No substrates: O-3-deoxy-beta-D-glucopyranosyl-(1->2)-D-glucopyranose, O-3-deoxy-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose
Products: -
Products: -
?
additional information
?
-
Substrates: the 3'-hydroxy group of sophorose is important for substrate recognition. No substrates: O-3-deoxy-beta-D-glucopyranosyl-(1->2)-D-glucopyranose, O-3-deoxy-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose
Products: -
Products: -
?
additional information
?
-
Substrates: the 3'-hydroxy group of sophorose is important for substrate recognition. No substrates: O-3-deoxy-beta-D-glucopyranosyl-(1->2)-D-glucopyranose, O-3-deoxy-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose
Products: -
Products: -
?
additional information
?
-
Listeria innocua Clip11262 ATCC BAA-680
Substrates: the 3'-hydroxy group of sophorose is important for substrate recognition. No substrates: O-3-deoxy-beta-D-glucopyranosyl-(1->2)-D-glucopyranose, O-3-deoxy-beta-D-glucopyranosyl-(1->2)-3-deoxy-D-glucofuranose
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n + phosphate
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
Substrates: the enzyme catalyses reversible the phosphorolysis of beta-(1->2)-D-glucans. The minimum length of the substrate for the phosphorolytic reaction is 3 glucose units. In the synthetic reaction starting from sophorose and alpha-D-glucose 1-phosphate the average polymerisation degree is 39
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the average product shows a polymerisation degree of 39 glucose units
Products: -
Products: -
r
[(1->2)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->2)-beta-D-glucosyl]n + phosphate
Substrates: the average product shows a polymerisation degree of 39 glucose units
Products: -
Products: -
r
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.8 - 2.5
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
1.2 - 6.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
5.5
2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
8.5
sophorose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
1.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
2.5
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
1.2
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
1.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
2.2
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
6.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
1
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
2.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
4.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
6
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
31 - 48
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
30 - 90
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
40
2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
97
sophorose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
31
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
48
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
30
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
42
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
46
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
90
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
47
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
55
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
110
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
17 - 19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
13 - 24
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
7.3
2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
11
sophorose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
17
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
13
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
21
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
23
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
24
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
9.8
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
phosphorolysis, pH 7, 40°C
18
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate alpha-D-glucopyranose 1-phosphate
19
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
pH 7.5, 30°C, cosubstrate phosphate, release of alpha-D-glucopyranose 1-phosphate
20
2-O-beta-D-glucopyranosyl-2-O-beta-D-glucopyranosyl-D-glucopyranose
synthesis, pH 7, 40°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 12OLP_LISIN
Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
1086
0
122895
Swiss-Prot
-
A9KJS6_LACP7
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
1113
0
126332
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the tertiary structure consists of two N-terminal beta-sandwich domains, a catalytic (alpha/alpha)6 barrel domain, and a beta sheet domain. Sophorose binds at subsites +1 to +2. The Glc moiety at subsite +1 is flipped compared with the corresponding ligands in other GH94 enzymes
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
synthesis of beta-1,2-glucan with sophorose as an acceptor through three synthesis steps using sucrose phosphorylase and 1,2-beta-oligoglucan phosphorylase. The first step is performed in 200 microl reaction solution containing 5 mM sophorose and 1.0 M sucrose. beta-1,2-Glucan in a part of the resultant solution is hydrolyzed to beta-1,2-glucooligosaccharides by beta-1,2-glucanase. The second synthesis is performed in 25 times the volume for the first synthesis. The hydrolysate solution (1% volume of the reaction solution) is used as an acceptor. After treatment with the beta-1,2-glucanase again, the third synthesis is performed in 200 times the volume of the second synthesis (1 l). More than 140 g of beta-1,2-glucan is synthesized using approximately 20 microg of sophorose as the starting acceptor material
synthesis
-
a method is developed to synthesize sophorose using three enzymes sucrose phosphorylase (cf. EC 2.4.1.7) from Leuconostoc mesenteroides, 1,2-beta-oligoglucan phosphorylase from Enterococcus italicus, and exo beta-1,2-glucooligosaccharide sophorohydrolase (cf. EC 3.2.1.214) from Parabacteroides distasonis in a one-pot reaction, employing inexpensive starting materials. After optimization, a reaction is carried out using 5 mM glucose, 250 mM sucrose, 10 mM inorganic phosphate, and enzyme concentrations of 0.005 mg/ml sucrose phosphorylase, 0.020 mg/ml 1,2-beta-oligoglucan phosphorylase, and 0.050 mg/ml exo beta-1,2-glucooligosaccharide sophorohydrolase at 30°C for 48 h, yielding 108 mM sophorose. Sophorose is purified with a final yield of 45% based on the amount of sucrose used as the donor substrate
synthesis
Listeria innocua Clip11262 ATCC BAA-680
-
synthesis of beta-1,2-glucan with sophorose as an acceptor through three synthesis steps using sucrose phosphorylase and 1,2-beta-oligoglucan phosphorylase. The first step is performed in 200 microl reaction solution containing 5 mM sophorose and 1.0 M sucrose. beta-1,2-Glucan in a part of the resultant solution is hydrolyzed to beta-1,2-glucooligosaccharides by beta-1,2-glucanase. The second synthesis is performed in 25 times the volume for the first synthesis. The hydrolysate solution (1% volume of the reaction solution) is used as an acceptor. After treatment with the beta-1,2-glucanase again, the third synthesis is performed in 200 times the volume of the second synthesis (1 l). More than 140 g of beta-1,2-glucan is synthesized using approximately 20 microg of sophorose as the starting acceptor material
-
synthesis
-
a method is developed to synthesize sophorose using three enzymes sucrose phosphorylase (cf. EC 2.4.1.7) from Leuconostoc mesenteroides, 1,2-beta-oligoglucan phosphorylase from Enterococcus italicus, and exo beta-1,2-glucooligosaccharide sophorohydrolase (cf. EC 3.2.1.214) from Parabacteroides distasonis in a one-pot reaction, employing inexpensive starting materials. After optimization, a reaction is carried out using 5 mM glucose, 250 mM sucrose, 10 mM inorganic phosphate, and enzyme concentrations of 0.005 mg/ml sucrose phosphorylase, 0.020 mg/ml 1,2-beta-oligoglucan phosphorylase, and 0.050 mg/ml exo beta-1,2-glucooligosaccharide sophorohydrolase at 30°C for 48 h, yielding 108 mM sophorose. Sophorose is purified with a final yield of 45% based on the amount of sucrose used as the donor substrate
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakajima, M.; Toyoizumi, H.; Abe, K.; Nakai, H.
Taguchi, H.; Kitaoka, M.: 1,2-beta-Oligoglucan phosphorylase from Listeria innocua
PLoS ONE
9
e92353
2014
Listeria innocua (Q92AT0), Listeria innocua, Listeria innocua CLIP 11262 (Q92AT0)
brenda
Kobayashi, K.; Nakajima, M.; Aramasa, H.; Kimura, S.; Iwata, T.; Nakai, H.; Taguchi, H.
Large-scale preparation of beta-1,2-glucan using quite a small amount of sophorose
Biosci. Biotechnol. Biochem.
83
1867-1874
2019
Listeria innocua Clip11262 (Q92AT0), Listeria innocua Clip11262 ATCC BAA-680 (Q92AT0)
brenda
Tanaka, N.; Nakajima, M.; Aramasa, H.; Nakai, H.; Taguchi, H.; Tsuzuki, W.; Komba, S.
Synthesis of three deoxy-sophorose derivatives for evaluating the requirement of hydroxy groups at position 3 and/or 3 of sophorose by 1,2-beta-oligoglucan phosphorylases
Carbohydr. Res.
468
13-22
2018
Listeria innocua Clip11262 (Q92AT0), Lachnoclostridium phytofermentans (A9KJS6), Lachnoclostridium phytofermentans DSM 18823 (A9KJS6), Listeria innocua Clip11262 ATCC BAA-680 (Q92AT0)
brenda
Nakajima, M.; Tanaka, N.; Furukawa, N.; Nihira, T.; Kodutsumi, Y.; Takahashi, Y.; Sugimoto, N.; Miyanaga, A.; Fushinobu, S.; Taguchi, H.; Nakai, H.
Mechanistic insight into the substrate specificity of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans
Sci. Rep.
7
42671
2017
Lachnoclostridium phytofermentans (A9KJS6), Lachnoclostridium phytofermentans DSM 18823 (A9KJS6)
brenda
Nakajima, M.
beta-1,2-Glucans and associated enzymes
Biologia
78
1741-1757
2023
Listeria innocua (Q92AT0), Lachnoclostridium phytofermentans (A9KJS6), Lachnoclostridium phytofermentans ATCC 700394 (A9KJS6), Listeria innocua ATCC BAA-680 (Q92AT0)
-
brenda
Tatebe, Y.; Yamamoto, Y.; Isono, N.
One-pot enzymatic synthesis of sophorose from sucrose and glucose
J. Appl. Glycosci. (1999)
72
7201201
2025
Enterococcus italicus, Enterococcus italicus DSM 15952
brenda
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