Literature summary for 2.4.1.31 extracted from

Kuhaudomlarp, S.; Walpole, S.; Stevenson, C.E.M.; Nepogodiev, S.A.; Lawson, D.M.; Angulo, J.; Field, R.A.
Unravelling the specificity of laminaribiose phosphorylase from Paenibacillus sp. YM-1 towards donor substrates glucose/mannose 1-phosphate by using X-ray crystallography and saturation transfer difference NMR spectroscopy (2019), ChemBioChem, 20, 181-192 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Paenibacillus sp. YM-1

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol 3350, 0.1 M Tris-citrate buffer, pH 6.0, 0.3 M ammonium sulfate	Paenibacillus sp. YM-1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.8	-	alpha-D-mannose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
4.2	-	alpha-D-glucose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
6.04	-	D-glucose	at pH 7.0 and 45°C	Paenibacillus sp. YM-1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
240000	-	gel filtration	Paenibacillus sp. YM-1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-D-glucose 1-phosphate + D-glucose	Paenibacillus sp. YM-1	-	laminaribiose + phosphate	-	r
alpha-D-mannose 1-phosphate + D-glucose	Paenibacillus sp. YM-1	-	beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus sp. YM-1	D7UT17	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex S200 gel filtration	Paenibacillus sp. YM-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate + D-glucose	-	Paenibacillus sp. YM-1	laminaribiose + phosphate	-	r
alpha-D-mannose 1-phosphate + D-glucose	-	Paenibacillus sp. YM-1	beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate	-	r
additional information	no activity with alpha-D-galactosamine 1-phosphate, alpha-D-glucosamine 1-phosphate, alpha-D-galacturonic acid 1-phosphate and alpha-D-galactose 1-phosphate	Paenibacillus sp. YM-1	?	-	-

Subunits

Subunits	Comment	Organism
homodimer	2 * 100000, SDS-PAGE	Paenibacillus sp. YM-1
homodimer	2 * 106000, calculated from amino acid sequence	Paenibacillus sp. YM-1

Synonyms

Synonyms	Comment	Organism
LBP	-	Paenibacillus sp. YM-1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.08	-	alpha-D-mannose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
13	-	alpha-D-glucose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
15.4	-	D-glucose	at pH 7.0 and 45°C	Paenibacillus sp. YM-1

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.02	-	alpha-D-mannose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
2.55	-	D-glucose	at pH 7.0 and 45°C	Paenibacillus sp. YM-1
3.07	-	alpha-D-glucose 1-phosphate	at pH 7.0 and 45°C	Paenibacillus sp. YM-1

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Release 2023.1 (January 2023)