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Information on EC 2.4.1.31 - laminaribiose phosphorylase
for references in articles please use BRENDA:EC2.4.1.31
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.31 laminaribiose phosphorylase
IUBMB Comments
Also acts on 1,3-β-D-oligoglucans. Differs in specificity from EC 2.4.1.30 (1,3-β-oligoglucan phosphorylase) and EC 2.4.1.97 (1,3-β-D-glucan phosphorylase).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
- 2.4.1.31
-
euglena
- phosphorylases
- gracilis
- glycoside
- 1-phosphate
-
phosphorolysis
- polymerization
-
bienzymatic
- oligosaccharide
-
sepabeads
-
biocatalysts
- glucan
- cellobiose
- laminaritriose
-
epoxy
- gps
- paenibacillus
Reaction Schemes
showSynonyms
laminaribiose phosphorylase, acl0729, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate
ordered bi-bi mechanism
-
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
laminaribiose degradation
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SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucosyl-D-glucose:phosphate alpha-D-glucosyltransferase
Also acts on 1,3-beta-D-oligoglucans. Differs in specificity from EC 2.4.1.30 (1,3-beta-oligoglucan phosphorylase) and EC 2.4.1.97 (1,3-beta-D-glucan phosphorylase).
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CAS REGISTRY NUMBER
COMMENTARY
37257-29-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
3-O-beta-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-arabino-hexopyranose
?
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
alpha-D-glucose 1-phosphate + D-glucuronic acid
3-O-beta-D-glucopyranosyl-D-glucuronic acid + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-mannose
3-O-beta-D-glucopyranosyl-D-mannose + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-xylose
?
Substrates: the major product (83%) in the reaction with D-xylose is 3-O-beta-D-glucopyranosyl-D-xylose and the other fraction (17%) contains 2-O-beta-D-glucopyranosyl-D-xylose as the major component
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + laminaritetraose
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + salicin
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-mannose 1-phosphate + D-glucose
beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
D-glucose + alpha-D-glucose 1-phosphate
3-beta-D-glucosyl-D-glucose + phosphate
-
Substrates: -
Products: -
Products: -
?
laminariheptaose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
laminarihexaose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
laminaripentaose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
laminaritetraose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
Astasia ocellata
-
Substrates: the enzyme is specific for the alpha-isomer
Products: -
Products: -
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
Astasia ocellata
-
Substrates: -
Products: oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose
Products: oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
Astasia ocellata
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Substrates: at equilibrium synthesis of laminaribiose is favoured
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
Astasia ocellata
-
Substrates: specific for alpha-D-glucose 1-phosphate as glucosyl donor
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
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Substrates: initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
Substrates: specific for alpha-D-glucose 1-phosphate as glucosyl donor
Products: amount of individual products depends on substrate concentration and reaction time
Products: amount of individual products depends on substrate concentration and reaction time
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + laminaribiose
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + laminaritriose
?
Astasia ocellata
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + laminaritriose
?
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
?
Astasia ocellata
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
?
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
?
Astasia ocellata
-
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
?
-
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: -
Products: -
?
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: -
Products: -
?
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Substrates: -
Products: -
Products: -
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Substrates: -
Products: -
Products: -
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
Substrates: -
Products: -
Products: -
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
laminaritriose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
laminaritriose + phosphate
?
Substrates: only weak phosphorylation
Products: -
Products: -
r
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
?
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
Substrates: -
Products: -
Products: -
r
additional information
?
-
Astasia ocellata
-
Substrates: in presence of arsenate, laminaribiose is completely converted into glucose
Products: -
Products: -
?
additional information
?
-
Astasia ocellata
-
Substrates: weak or no activity observed with other carbohydrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: EC 1.4.1.31 and EC 1.4.1.30 catalyse the same reaction but with different quantitative specificity, EC 1.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose while the opposite behaviour is observed with EC 1.4.1.31
Products: -
Products: -
?
additional information
?
-
-
Substrates: arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate
Products: -
Products: -
?
additional information
?
-
-
Substrates: weak or no activity observed with other carbohydrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: weak or no activity observed with other carbohydrates
Products: -
Products: -
?
additional information
?
-
Substrates: on reverse phosphorolysis enzyme is most active on glucose as acceptor and is weakly active on several monosaccharides (e.g. mannose) with alpha-D-glucose 1-phosphate as donor
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with alpha-D-galactosamine 1-phosphate, alpha-D-glucosamine 1-phosphate, alpha-D-galacturonic acid 1-phosphate and alpha-D-galactose 1-phosphate
Products: -
Products: -
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-mannose 1-phosphate + D-glucose
beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate
Substrates: -
Products: -
Products: -
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
Substrates: -
Products: -
Products: -
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
Substrates: -
Products: -
Products: -
r
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
p-hydroxymercuribenzoate
-
40% inhibition at 0.2 mM and 80% inhibition at 2 mM
additional information
-
not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
-
D-glucose
higher concentrations of glucose cause competitive substrate inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
linear within certain limits
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
reverse phosphorolytic reaction using alpha-D-glucose 1-phosphate as the donor and D-glucose as the acceptor has optimum at pH 6.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
metagenomic libraries screened for enzyme activity are sourced from a fecal sample of the North American beaver, Castor canadensis
-
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
200000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
homodimer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE