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Literature summary for 2.4.1.31 extracted from

  • Nihira, T.; Saito, Y.; Kitaoka, M.; Nishimoto, M.; Otsubo, K.; Nakai, H.
    Characterization of a laminaribiose phosphorylase from Acholeplasma laidlawii PG-8A and production of 1,3-beta-D-glucosyl disaccharides (2012), Carbohydr. Res., 361C, 49-54.
    View publication on PubMed
Search for more literature for 2.4.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Acholeplasma laidlawii

Inhibitors

Inhibitors Comment Organism Structure
laminaribiose
-
 Acholeplasma laidlawii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 phosphate pH 7.5, 40°C Acholeplasma laidlawii
0.4
-
 D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
0.7
-
 2-deoxy-D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
2.7
-
 laminaribiose pH 7.5, 40°C Acholeplasma laidlawii

Organism

Organism UniProt Comment Textmining
Acholeplasma laidlawii A9NG65
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
-
 Acholeplasma laidlawii 3-O-beta-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
alpha-D-glucose 1-phosphate + 2-deoxy-D-arabino-hexopyranose
-
 Acholeplasma laidlawii ?
-
 r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Acholeplasma laidlawii ?
-
 r
alpha-D-glucose 1-phosphate + D-glucose
-
 Acholeplasma laidlawii laminaribiose + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-glucuronic acid
-
 Acholeplasma laidlawii 3-O-beta-D-glucopyranosyl-D-glucuronic acid + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-mannose
-
 Acholeplasma laidlawii 3-O-beta-D-glucopyranosyl-D-mannose + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-xylose the major product (83%) in the reaction with D-xylose is 3-O-beta-D-glucopyranosyl-D-xylose and the other fraction (17%) contains 2-O-beta-D-glucopyranosyl-D-xylose as the major component Acholeplasma laidlawii ?
-
 r
laminaribiose + phosphate
-
 Acholeplasma laidlawii alpha-D-glucose 1-phosphate + D-glucose
-
 r
laminaritriose + phosphate only weak phosphorylation Acholeplasma laidlawii ?
-
 r

Synonyms

Synonyms Comment Organism
1,3-beta-D-glucobiose
-
 Acholeplasma laidlawii
ACL0729
-
 Acholeplasma laidlawii
laminaribiose phosphorylase
-
 Acholeplasma laidlawii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Acholeplasma laidlawii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable up to 35°C during 15 min incubation Acholeplasma laidlawii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 2-deoxy-D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
1.4
-
 D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
6.5
-
 laminaribiose pH 7.5, 40°C, co-substrate: phosphate Acholeplasma laidlawii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reverse phosphorolytic reaction using alpha-D-glucose 1-phosphate as the donor and D-glucose as the acceptor has optimum at pH 6.0 Acholeplasma laidlawii
7.5
-
 highest apparent phosphorolytic activity on laminaribiose Acholeplasma laidlawii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.5 stable at 4°C for 24h Acholeplasma laidlawii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4
-
 laminaribiose pH 7.5, 40°C Acholeplasma laidlawii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.009
-
 D-mannose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
0.024
-
 D-glucuronic acid pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
0.024
-
 1,5-anhydro-D-glucitol pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
0.064
-
 D-xylose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
1.4
-
 2-deoxy-D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii
3.5
-
 D-glucose pH 6.0, 40°C, reverse reaction Acholeplasma laidlawii