Literature summary for 2.4.1.255 extracted from

Alam, S.M.D.; Tsukamoto, Y.; Ogawa, M.; Senoo, Y.; Ikeda, K.; Tashima, Y.; Takeuchi, H.; Okajima, T.
N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization (2020), J. Biol. Chem., 295, 8560-8574 .

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Protein Variants

Protein Variants	Comment	Organism
N263Q/N354Q	a decrease in O-GlcNAc stoichiometry is observed in Notch1 co-expressed with an N263Q/N354Q variant compared with wild-type enzyme. The N263Q/N354Q variant exhibits altered subcellular distribution within the endoplasmic reticulum in HEK293T cells, indicating that N-glycosylation of EOGT is required for its localization in the endoplasmic reticulum at the cell periphery	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8BYW9	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	N-glycosylation sites Asn-263 and Asn-354, but not Asn-493, are modified with N-glycans. Both residues are modified with oligomannose N-glycans. Loss of an individual N-glycan does not affect endoplasmic reticulum localization of the enzyme (EOGT), enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK-293T cells. Simultaneous substitution of both N-glycosylation sites affects both enzyme maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for enzyme maturation and expression	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
EGF domain-specific O-GlcNAc transferase	-	Mus musculus
EOGT	-	Mus musculus
epidermal growth factor domain-specific O-GlcNAc transferase	-	Mus musculus

General Information

General Information	Comment	Organism
malfunction	several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database	Mus musculus

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Release 2023.1 (January 2023)