Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ADP-glucose | enzyme oligomer, pH not specified in the publication, temperature not specified in the publication | Oryza sativa | |
0.03 | - |
ADP-glucose | enzyme monomer, pH not specified in the publication, temperature not specified in the publication | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Oryza sativa Zh11 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation status is strongly enhanced by mitogen-activated protein kinase MAPK and ATP treatment and is sharply weakened by protein phosphatase treatment | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + (1,4-alpha-D-glucosyl)n | - |
Oryza sativa | ADP + (1,4-alpha-D-glucosyl)n+1 | - |
? | |
ADP-glucose + (1,4-alpha-D-glucosyl)n | - |
Oryza sativa Zh11 | ADP + (1,4-alpha-D-glucosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | and oligomer, gel filtration and SDS-PAGE | Oryza sativa |
oligomer | and monomer, gel filtration and SDS-PAGE | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
physiological function | GBSS1 can form oligomers in rice endosperm, and oligomerized OsGBSS1 exhibits much higher specific enzymatic activity than the monomer. A monomer-oligomer transition equilibrium occurs in the endosperm during development. Redox potential is a key factor affecting the oligomer percentage as well as the enzymatic activity. Adenosine diphosphate glucose, the direct donor of glucose, also impacts GBSS1 oligomerization in a concentration-dependent manner. GBSS1 oligomerization is influenced by phosphorylation status, which is strongly enhanced by mitogen-activated protein kinase MAPK and ATP treatment and is sharply weakened by protein phosphatase treatment | Oryza sativa |