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Literature summary for 2.4.1.214 extracted from

  • Mercx, S.; Smargiasso, N.; Chaumont, F.; De Pauw, E.; Boutry, M.; Navarre, C.
    Inactivation of the beta(1,2)-xylosyltransferase and the alpha(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 cells by a Multiplex CRISPR/Cas9 strategy results in glycoproteins without plant-specific glycans (2017), Front. Plant Sci., 8, 403 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.214

Application

Application Comment Organism
synthesis a KO line lacking two XylT genes and four FucT genes expressing a human IgG2 antibody shows an IgG2 expression level as high as in a control transformant. The IgG glycosylation profile shows no beta(1,2)-xylose or alpha(1,3)-fucose present on the glycosylation moiety. The dominant glycoform is the GnGn structure Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum A0A1S4AG82
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Nicotiana tabacum A0A1S4DHZ6
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Nicotiana tabacum D6RSA0
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Source Tissue

Source Tissue Comment Organism Textmining
TBY-2 cell
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 Nicotiana tabacum
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Synonyms

Synonyms Comment Organism
FucT
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 Nicotiana tabacum
FucTA
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 Nicotiana tabacum
FucTB
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 Nicotiana tabacum
FucTC
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 Nicotiana tabacum
LOC107797347 locus name Nicotiana tabacum
LOC107830046 locus name Nicotiana tabacum
NM_001324945
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 Nicotiana tabacum
XM_016620229
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 Nicotiana tabacum
XM_016657530
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 Nicotiana tabacum

General Information

General Information Comment Organism
physiological function knocking-out of two XylT genes and four FucT genes in BY-2 suspension cells using CRISPR/Cas9. Three lines show a strong reduction of beta(1,2)-xylose and alpha(1,3)-fucose, while two lines are completely devoid of them, indicating complete gene inactivation. The KO lines do not show any particular morphology and grow as the wild-type. A KO line transformed with genes encoding a human IgG2 antibody shows an IgG2 expression level as high as in a control transformant. The IgG glycosylation profile shows no beta(1,2)-xylose or alpha(1,3)-fucose present on the glycosylation moiety. The dominant glycoform is the GnGn structure Nicotiana tabacum