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Literature summary for 2.4.1.20 extracted from

  • Hidaka, M.; Kitaoka, M.; Hayashi, K.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    Structural dissection of the reaction mechanism of cellobiose phosphorylase (2006), Biochem. J., 398, 37-43.
    View publication on PubMed
Search for more literature for 2.4.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
at 25°C using the sitting-drop vapour diffusion method. Cellobiose phosphorylase-SO4 and cellobiose phosphorylase-PO4 structures are determined at 2.0 and 2.1 A resolution to R factor (Rfree) of 17.6% (21.3%) and 18.0% (22%) respectively. The pH values for crystallization are pH 8.2 for cellobiose phosphorylase-PO4 and pH 6.5 for cellobiose phosphorylase-SO4. Cellulomonas gilvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8 9 1,5-anhydroglucitol enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
71
-
 isomaltose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
83
-
 gentiobiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
93
-
 melibiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus

Organism

Organism UniProt Comment Textmining
Cellulomonas gilvus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellulomonas gilvus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydroglucitol + glucose-1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
gentiobiose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
isomaltose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
melibiose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Cellulomonas gilvus

Synonyms

Synonyms Comment Organism
Cbp
-
 Cellulomonas gilvus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 isomaltose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.3
-
 melibiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
3.5
-
 1,5-anhydroglucitol enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
7.2
-
 gentiobiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus