Cloned (Comment) | Organism |
---|---|
gene Pig-a, DNA and amino acid sequence determination and analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | GPI anchor deficiency is most often caused by mutation in the Pig-a gene, thus measuring GPI anchor deficiency is considered to be almost equivalent to measuring Pig-a mutation. Development and validation of an in vitro Pig-a assay in L5178Y mouse lymphoma cells, method optimization, overview. Antibodies against GPI-linked CD90.2 and stably expressed CD45 are used to determine GPI-status by flow cytometry, antibody concentration and incubation times are optimized. The optimum phenotypic expression period is 8 days. Application of the in vitro Pig-a assay to a selection of well-validated genotoxic and non-genotoxic compounds. EMS, N-ethyl-N-nitrosourea, and 4-nitroquinoline-N-oxide dose dependently increase the numbers of GPI(-) cells, while etoposide, mitomycin C, and a bacterial-specific mutagen do not dependently increase the numbers of GPI(-) cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | Mus musculus | - |
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q64323 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
L-5178-Y cell | mouse lymphoma cells | Mus musculus | - |
lymphoma cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | - |
Mus musculus | UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PigA | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations in Pig-a prevent GPI-anchor synthesis resulting in loss of cell-surface GPI-linked proteins | Mus musculus |
metabolism | the enzyme is involved in the glycophosphatidylinositol (GPI) anchor synthesis pathway which is highly conserved between species | Mus musculus |
physiological function | hemizygous phosphatidylinositol class A (Pig-a) forms the catalytic subunit of N-acetylglucosaminyltransferase that is required for glycophosphatidylinositol (GPI) anchor biosynthesis | Mus musculus |