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Literature summary for 2.4.1.173 extracted from

  • Singh, G.; Tiwari, M.; Singh, S.P.; Singh, S.; Trivedi, P.K.; Misra, P.
    Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera (2016), Sci. Rep., 6, 25562 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.173

Protein Variants

Protein Variants Comment Organism
additional information analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifer. Phenotype, overview Withania somnifera
additional information analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifera. Phenotype, overview Withania somnifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + a sterol Withania somnifera
-
 UDP + a sterol 3-beta-D-glucoside
-
 ?
UDP-glucose + beta-sitosterol Withania somnifera
-
 UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
UDP-glucose + stigmasterol Withania somnifera
-
 UDP + stigmasterol 3-beta-D-glucoside
-
 ?
UDP-glucose + withaferin A Withania somnifera
-
 UDP + ?
-
 ?
UDP-glucose + withanolide A Withania somnifera
-
 UDP + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Withania somnifera
-
-
-
Withania somnifera Q2I014
-
-
Withania somnifera Q2I015
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Withania somnifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + a sterol
-
 Withania somnifera UDP + a sterol 3-beta-D-glucoside
-
 ?
UDP-glucose + beta-sitosterol
-
 Withania somnifera UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
UDP-glucose + stigmasterol
-
 Withania somnifera UDP + stigmasterol 3-beta-D-glucoside
-
 ?
UDP-glucose + withaferin A
-
 Withania somnifera UDP + ?
-
 ?
UDP-glucose + withanolide A
-
 Withania somnifera UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
SGTL1
-
 Withania somnifera
SgtL2
-
 Withania somnifera
SgtL4
-
 Withania somnifera
sterol glycosyltransferase
-
 Withania somnifera

General Information

General Information Comment Organism
malfunction analysis of the function of SGTs by silencing SGTL1, SGTL2 and SGTL4 in Withania somnifera. Downregulation of SGTs by artificial miRNAs leads to the enhanced accumulation of withanolide A, withaferin A, sitosterol, stigmasterol and decreased content of withanoside V in virus induced gene silencing (VIGS) lines. This is further correlated with increased expression of WsHMGR, WsDXR, WsFPPS, WsCYP710A1, WsSTE1 and WsDWF5 genes, involved in withanolide biosynthesis. These variations of withanolide concentrations in silenced lines result in pathogen susceptibility as compared to control plants. The infection of Alternaria alternata causes increased salicylic acid, callose deposition, superoxide dismutase and H2O2 in aMIR-VIGS lines. The expression of biotic stress related genes, namely, WsPR1, WsDFS, WsSPI and WsPR10 is also enhanced in aMIR-VIGS lines in time dependent manner. Salicylic acid level increases the expression of defence related genes in silenced lines. A positive feedback regulation of withanolide biosynthesis occurs by silencing of SGTLs which results in reduced biotic tolerance Withania somnifera
physiological function sterol glycosyltransferases (SGTs) catalyse transfer of glycon moiety to sterols and their related compounds to produce diverse glyco-conjugates or steryl glycosides with different biological and pharmacological activities. SGTs from Withania somnifera play a role in abiotic stresses Withania somnifera