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Literature summary for 2.4.1.17 extracted from

  • Davis, B.D.; Brodbelt, J.S.
    Regioselectivity of human UDP-glucuronosyl-transferase 1A1 in the synthesis of flavonoid glucuronides determined by metal complexation and tandem mass spectrometry (2008), J. Am. Soc. Mass Spectrom., 19, 246-256.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.17

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ten flavonoid aglycons are used as substrates, spanning the subclasses of flavones, flavonols, and flavanones. The products are characterized by LC-MS and LC-MSn, with post-column cobalt complexation employed to pinpoint the specific sites of conjugation. The dissociation of complexes of the form [Co(II) (flavonoid glucuronide-H) (4,7-diphenyl-1,10-phenanthroline)2]+ allow identification of the products and differentiation of isomers. The correlation between glycosylation site and elution order is used to provide additional structural confirmation. Flavonoids lacking a 3' hydroxyl group are glucuronidated only at position 7, while those containing this functionality also form 3'-O-glucuronides and sometimes 4'-O-glucuronides, thus supporting the conclusion that the presence or absence of the 3'-OH group is the major determinant of the regioselectivity of glucuronidation. Moreover, the specific distribution of multiple glucuronide products, 7-O, 3'-O, 4'-O, is governed by the subclass of flavonoid, structure-activity relationships, overview Homo sapiens ?
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 ?
UDP-D-glucuronate + acacetin
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 Homo sapiens UDP + acacetin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + acceptor
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 Homo sapiens UDP + acceptor beta-D-glucuronoside
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 ?
UDP-D-glucuronate + apigenin
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 Homo sapiens UDP + apigenin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + eriodictyol
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 Homo sapiens UDP + eriodictyol 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + hesperetin
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 Homo sapiens UDP + hesperetin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + homoeriodictyol
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 Homo sapiens UDP + homoeriodictyol 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + kaempferol
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 Homo sapiens UDP + kaempferol 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + luteolin
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 Homo sapiens UDP + luteolin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + myricetin
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 Homo sapiens UDP + myricetin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + naringenin
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 Homo sapiens UDP + naringenin 7-O-beta-D-glucuronoside
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 ?
UDP-D-glucuronate + quercetin
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 Homo sapiens UDP + quercetin 7-O-beta-D-glucuronoside
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 ?

Synonyms

Synonyms Comment Organism
UDP-glucuronosyl-transferase 1A1
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 Homo sapiens
UGT1A1
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 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens