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Literature summary for 2.4.1.16 extracted from

  • Gooday, G.W.; de Rousset-Hall, A.
    Properties of chitin synthetase from Coprinus cinereus (1975), J. Gen. Microbiol., 89, 137-145.
No PubMed abstract available
Search for more literature for 2.4.1.16

General Stability

General Stability Organism
treatment with digitonin causes an increase in specific activity and stability Coprinopsis cinerea

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Coprinopsis cinerea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stipe
-
 Coprinopsis cinerea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
-
 Coprinopsis cinerea

Storage Stability

Storage Stability Organism
-20°C, solubilized preparations show no loss of activity after 5 weeks Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Coprinopsis cinerea UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Coprinopsis cinerea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
9 43 50% of maximal activity at 9°C and at 43°C Coprinopsis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 2 h, solubilized preparations retain 80% of its activity Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Coprinopsis cinerea