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Literature summary for 2.4.1.16 extracted from

  • Machida, S.; Saito, M.
    Purification and characterization of membrane-bound chitin synthase (1993), J. Biol. Chem., 268, 1702-1707.
    View publication on PubMed
Search for more literature for 2.4.1.16

Activating Compound

Activating Compound Comment Organism Structure
GlcNAc stimulates Absidia glauca

Inhibitors

Inhibitors Comment Organism Structure
Cl- 10 mM Absidia glauca
Co2+ 10 mM CoCl2, 20% inhibition Absidia glauca
Cu2+ 10 mM CuSO4, 95% inhibition Absidia glauca
Polyoxin D 0.01 mM, 80% loss of activity; 0.1 mM, complete inhibition Absidia glauca
SO42- 10 mM Absidia glauca
UDP 0.05 mM, 50% loss of activity; 0.1 mM, 95% inhibition Absidia glauca
Zn2+ 10 mM ZnCl2, 95% inhibition Absidia glauca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 UDP-GlcNAc
-
 Absidia glauca

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Absidia glauca
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Absidia glauca
Mn2+ slight activation Absidia glauca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Absidia glauca
520000
-
 gel filtration Absidia glauca
540000
-
 calculation from sedimentation data Absidia glauca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n Absidia glauca key enzyme in chitin biosynthesis UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Absidia glauca
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Absidia glauca
proteolytic modification activity increases up to 20times by digestion with trypsin Absidia glauca

Purification (Commentary)

Purification (Comment) Organism
-
 Absidia glauca

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Absidia glauca
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0055
-
-
 Absidia glauca

Storage Stability

Storage Stability Organism
-80°C, 20% glycerol, stable Absidia glauca
4°C, 20% glycerol, 50% loss of activity after several days Absidia glauca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Absidia glauca UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n key enzyme in chitin biosynthesis Absidia glauca UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Subunits

Subunits Comment Organism
polymer x * 30000, SDS-PAGE Absidia glauca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Absidia glauca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, complete loss of activity Absidia glauca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Absidia glauca

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: 40% of maximal activity, pH 8.0: 95% of maximal activity Absidia glauca