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Literature summary for 2.4.1.16 extracted from

  • Sburlati, A.; Cabib, E.
    Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae (1986), J. Biol. Chem., 261, 15147-15152.
    View publication on PubMed
Search for more literature for 2.4.1.16

Activating Compound

Activating Compound Comment Organism Structure
GlcNAc stimulates both Chs1 and Chs2 about 3fold Saccharomyces cerevisiae
papain fairly good activator of Chs1, poor activator of Chs2 Saccharomyces cerevisiae
Pronase stimulates both Chs1 and Chs2 Saccharomyces cerevisiae
proteinase B from Saccharomyces cerevisiae, stimulates Chs1, no effect on Chs2 Saccharomyces cerevisiae
proteinase K stimulates both Chs1 and Chs2 Saccharomyces cerevisiae
Staphylococcus V8 protease best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibits Chs1 Saccharomyces cerevisiae
EDTA 0.1 mM, Chs1 and Chs2 are almost completely inhibited Saccharomyces cerevisiae
NaCl 0.5 M, 86% inhibition of Chs1 and 29% inhibition of Chs2 Saccharomyces cerevisiae
Polyoxin D Chs2 shows less sensitivity to inhibition than Chs1 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 UDP-GlcNAc Chs1 Saccharomyces cerevisiae
0.9
-
 UDP-GlcNAc Chs1 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane most of the enzyme is associated with Saccharomyces cerevisiae 5886
-
protoplast
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ in stimulation of Chs2 Co2+ is twice as effective on an enzyme activated by trypsin Saccharomyces cerevisiae
Mg2+ best stimulator of Chs1. Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 Saccharomyces cerevisiae
Mn2+ Mg2+ and Mn2+ lead to similar maximal stimulation of Chs2 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n Saccharomyces cerevisiae chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 chitin synthetase 2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification pronase and proteinase K stimulates both Chs1 and Chs2. Proteinase B from Saccharomyces cerevisiae stimulates Chs1 and has no effect on Chs2, Staphylococcus V8 protease is the best activator of Chs2 in presence of Co2+, elicits little Mg2+-stimulatable activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 chitin ?
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n chitin synthetase 2 is the physiological agent for chitin deposition in strains with a disrupted CHS1 gene Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 Polyoxin D Chs1 Saccharomyces cerevisiae
0.015
-
 Polyoxin D Chs2 Saccharomyces cerevisiae