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Literature summary for 2.4.1.152 extracted from

  • Bai, J.; Wu, Z.; Sugiarto, G.; Gadi, M.R.; Yu, H.; Li, Y.; Xiao, C.; Ngo, A.; Zhao, B.; Chen, X.; Guan, W.
    Biochemical characterization of Helicobacter pylori alpha1-3-fucosyltransferase and its application in the synthesis of fucosylated human milk oligosaccharides (2019), Carbohydr. Res., 480, 1-6 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.422
-
 N-acetyllactosamine pH 7.5, 37°C Helicobacter pylori
3.729
-
 lactose pH 7.5, 37°C Helicobacter pylori
5.3392
-
 GDP-beta-fucose cosubstrate lactose, pH 7.5, 37°C Helicobacter pylori
6.448
-
 GDP-beta-fucose cosubstrate N-acetyllactosamine, pH 7.5, 37°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal cation required Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O30511
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-fucose + lacto-N-neotetraose
-
 Helicobacter pylori GDP + Galbeta1-4(Fucalpha1-3)GlcNAc
-
 ?
GDP-beta-fucose + lactose N-acetyllactosamine is preferred over lactose Helicobacter pylori GDP + alpha1,3-fucosyllactose
-
 ?
GDP-beta-fucose + N-acetyllactosamine N-acetyllactosamine is preferred over lactose. At a low GDP-fucose to acceptor ratio, the enzyme selectively fucosylates N-acetyllactosamine Helicobacter pylori GDP + alpha1,3-fucosyl-N-acetyllactosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 44200, SDS-PAGE of recombinant His-tagged protein Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 lactose pH 7.5, 37°C Helicobacter pylori
0.043
-
 GDP-beta-fucose cosubstrate lactose, pH 7.5, 37°C Helicobacter pylori
0.367
-
 N-acetyllactosamine pH 7.5, 37°C Helicobacter pylori
0.617
-
 GDP-beta-fucose cosubstrate N-acetyllactosamine, pH 7.5, 37°C Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5
-
 Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0054
-
 lactose pH 7.5, 37°C Helicobacter pylori
0.0079
-
 GDP-beta-fucose cosubstrate lactose, pH 7.5, 37°C Helicobacter pylori
0.096
-
 GDP-beta-fucose cosubstrate N-acetyllactosamine, pH 7.5, 37°C Helicobacter pylori
0.87
-
 N-acetyllactosamine pH 7.5, 37°C Helicobacter pylori