Literature summary for 2.4.1.152 extracted from

Choi, Y.H.; Kim, J.H.; Park, B.S.; Kim, B.G.
Solubilization and Iterative Saturation Mutagenesis of alpha1,3-fucosyltransferase from Helicobacter pylori to enhance its catalytic efficiency (2016), Biotechnol. Bioeng., 113, 1666-1675 .

Search for more literature for 2.4.1.152

Application

Application	Comment	Organism
synthesis	to overcome the poor solubility of FutA upon expression in Escherichia coli, codon optimization, and systematic truncation of the protein at the C-terminus with only one heptad repeat remaining yield 150-200 mg/l of soluble protein of FutA and result in more than an 18fold increase in the 3-fucosyllactose yield. Mutant A128N obtained by focused directed evolution mutant displays a 3.4fold higher catalytic activity than wild-type FutA. Mutant A128N/H129E/Y132I exhibits a 9.6fold improvement in specific activity when compared to wild-type. Mutants A128N/H129E/S46F and A128N/H129E/Y132I/S46F show synergistic effects, that is 14.5- and 15.5fold improvement in specific activity relative to wild-type. The mutations increase the binding affinity for lactose and kcat values for lactose and GDP-fucose. The quadruple mutant A128N/H129E/Y132I/S46F synthesizes 3-fucosyllactose with an improved yield and productivity (more than 96% yield based on 5 mM of GDP-Fuc within 1 h)	Helicobacter pylori

Cloned(Commentary)

Cloned (Comment)	Organism
expresssion in Escherichia coli	Helicobacter pylori

Protein Variants

Protein Variants	Comment	Organism
A128N	mutant obtained by focused directed evolution mutant, 3.4fold higher catalytic activity than wild-type	Helicobacter pylori
A128N/H129E/S46F	14.5fold improvement in specific activity	Helicobacter pylori
A128N/H129E/Y132I	9.6fold improvement in specific activity	Helicobacter pylori
A128N/H129E/Y132I/S46F	15.5fold improvement in specific activity	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	O25142	-	-
Helicobacter pylori 26695	O25142	-	-

Synonyms

Synonyms	Comment	Organism
FutA	-	Helicobacter pylori
HP_0379	-	Helicobacter pylori

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Release 2023.1 (January 2023)