Literature summary for 2.4.1.143 extracted from

Miyazaki, T.; Kato, T.; Park, E.Y.
Heterologous expression, purification and characterization of human beta-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system (2018), J. Biosci. Bioeng., 126, 15-22 .

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Cloned(Commentary)

Cloned (Comment)	Organism
construction of a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system, and functional expression of His-FLAG-tagged human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region. The enzyme is secreted. The enzyme expressed in silkworm is glycosylated, the structure of N-glycans in the recombinant hGnTII is suggested to be of the complex type, pauci-mannosidic-type glycans, and the removal of the glycans does not affect the enzymatic activity	Homo sapiens

Cloned (Comment)

Organism

construction of a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system, and functional expression of His-FLAG-tagged human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region. The enzyme is secreted. The enzyme expressed in silkworm is glycosylated, the structure of N-glycans in the recombinant hGnTII is suggested to be of the complex type, pauci-mannosidic-type glycans, and the removal of the glycans does not affect the enzymatic activity

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of an enzyme mutant HF-hGnTIIDELTA29 lacking the N-terminal cytosolic tail and transmembrane region	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi membrane	GnTII is a Golgi-localized type II transmembrane enzyme	Homo sapiens	139	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q10469	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	analysis of N-glycan attached to HF-hGnTIIDELTA29 mutant enzyme recombinantly expressed in silkworm using Endoglycosidase H (Endo H) and peptide:N-glycanase F (PNGase F)	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble His-FLAG-tagged hGnTII from silkworm larval hemolymph in tandem affinity purification and ultrafiltration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the recombinant enzyme exhibits glycosyltransferase activity and strict substrate specificity. The acceptor substrate specificity of enzyme mutant HF-hGnTIIDELTA29 is examined using UDP-GlcNAc as a donor substrate and MM-PA, GnM-PA, GnGn-PA, and p-nitrophenyl-alpha-mannopyranoside as acceptor substrates instead of MGn-PA, overview	Homo sapiens	?	-	-

Synonyms

Synonyms	Comment	Organism
beta-1,2-N-acetylglucosaminyltransferase II	-	Homo sapiens
GnTII	-	Homo sapiens
Mgat2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	beta-1,2-N-acetylglucosaminyltransferase II (GnTII) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans	Homo sapiens