Any feedback?
Information on EC 2.4.1.143 - alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
for references in articles please use BRENDA:EC2.4.1.143Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.4.1.143 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseIUBMB Comments
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its activity initiates the synthesis of the second antenna of di-antennary complex N-glycans. While the natural substrate (produced by EC 3.2.1.114, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->6)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]-beta-D-Man-R.
Specify your search results
Word Map
- 2.4.1.143
- n-glycans
-
carbohydrate-deficient
-
complex-type
-
dyserythropoietic
-
oligomannose
- hempas
-
schachter
-
monoantennary
- phosphomannomutase
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
=
+
+
=
+
Synonyms
gntii, n-acetylglucosaminyltransferase ii, gnt ii, glcnac-t ii, gnt-ii, beta-1,2-n-acetylglucosaminyltransferase ii, gnt ii-a, gnt ii-b, tbgntii, n-acetylglucosaminyltransferase-ii, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase II
-
-
-
-
alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
-
-
-
-
alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase
-
-
-
-
beta-1,2-N-acetylglucosaminyltransferase II
EC 2.4.1.51
-
-
formerly, part transferred
-
GlcNAcT-II
GnT II
GnT-II
GnTII
Mgat2
N-acetylglucosaminyltransferase II
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
-
-
-
-
TbGnTII
TbGT15
UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II
-
UDP-GlcNAc:mannoside alpha1-6 acetylglucosaminyltransferase
-
-
-
-
uridine diphosphoacetylglucosamine-alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase
-
-
-
-
uridine diphosphoacetylglucosamine-alpha-D-mannoside beta1-2-acetylglucosaminyltransferase
-
-
-
-
uridine diphosphoacetylglucosamine-mannoside alpha1->6-acetylglucosaminyltransferase
-
-
-
-
N-acetylglucosaminyltransferase II
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its activity initiates the synthesis of the second antenna of di-antennary complex N-glycans. While the natural substrate (produced by EC 3.2.1.114, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->6)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]-beta-D-Man-R.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
105913-04-0
-
202420-38-0
-
91755-74-7
-
91847-11-9
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-Man-(1->6)-[beta-D-GlcNAc(1->2)-alpha-D-Man(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + UDP-GlcNAc
?
i.e. pyridylaminated MGn glycan, an intermediate during N-glycan processing
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
?
Gbeta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn glycan acceptor (GlcNAc-Man3-GlcNAc2-Asn) is used as acceptor substrate in enzyme assay, generated by enzymatic modification of a sialylglycopeptide (SGP) isolated from hen egg yolks and purified
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP-GlcNAc + GlcNAcMan3GlcNAc2-pyridylamine
UDP + GlcNAc2Man3GlcNAc2-pyridylamine
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 3-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)3-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 3-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(3-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 3-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)3-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)4-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(4-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP-N-acetyl-D-glucosamine + 4-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 6-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)6-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 6-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(6-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 6-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)6-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-2-aminopyridine
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-2-aminopyridine
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
the enzyme catalyzes an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
essential for biosynthesis of complex N-linked oligosaccharides
-
?
UDP-N-acetyl-D-glucosamine + dideoxytetrasaccharide
UDP + dideoxypentasaccharide
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)-6-deoxy-Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)-6-deoxy-Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))-4-deoxy-Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))-4-deoxy-Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP-N-acetyl-D-glucosamine + Manalpha(1->6)[GlcNAcbeta(1->2)-Manbeta(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridyl-aminoside
?
-
the enzyme can transfer a second N-acetylglucosamine residue to the product of the first transferase reaction but in an extremely slow reaction that is unlikely to be biologically relevant
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
-
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->3)-alpha-D-Man-(1->6)-alpha-D-Man
?
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->3)-alpha-D-Man-(1->6)-alpha-D-Man
?
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(4-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(4-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
or 1,4-(+/-)-fucose-1,6-N-acetylglucosamine-pyridinylamine (with or without xylose in 1,2-beta-linkage to the beta-mannosyl residue)
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
R represents the remainder of the N-oligosaccharide core (n fucose), R: H or 1,4-(+/-)-fucose-1,6-N-acetylglucosaminyl-1-N-asparagine
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
acceptors are free and protein-matrix-bound glycans
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
best acceptor, high specificity
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
UDP-N-acetyl-D-glucosamine cannot be replaced by UDP-N-acetylgalactosamine, UDPgalactose, UDPxylose, UDPglucose or GDPfucose
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
no acceptor substrates: overview
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
-
?
additional information
?
-
-
the gene is alternatively spliced and developmmentally regulated
-
-
?
additional information
?
-
-
the enzyme is required for the chain initiation and elongation of the heparan sulfate backbone
-
-
?
additional information
?
-
binding structure of acceptor substrate and UDP, overview
-
-
-
additional information
?
-
-
binding structure of acceptor substrate and UDP, overview
-
-
-
additional information
?
-
the recombinant enzyme exhibits glycosyltransferase activity and strict substrate specificity. The acceptor substrate specificity of enzyme mutant HF-hGnTIIDELTA29 is examined using UDP-GlcNAc as a donor substrate and MM-PA, GnM-PA, GnGn-PA, and p-nitrophenyl-alpha-mannopyranoside as acceptor substrates instead of MGn-PA, overview
-
-
-
additional information
?
-
-
the recombinant enzyme exhibits glycosyltransferase activity and strict substrate specificity. The acceptor substrate specificity of enzyme mutant HF-hGnTIIDELTA29 is examined using UDP-GlcNAc as a donor substrate and MM-PA, GnM-PA, GnGn-PA, and p-nitrophenyl-alpha-mannopyranoside as acceptor substrates instead of MGn-PA, overview
-
-
-
additional information
?
-
-
the enzyme cannot use Man3GlcNAc2 and Manalpha(1->6)[Manbeta(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridyl-aminoside as substrate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-Man-(1->6)-[beta-D-GlcNAc(1->2)-alpha-D-Man(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + UDP-GlcNAc
?
i.e. pyridylaminated MGn glycan, an intermediate during N-glycan processing
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
the enzyme catalyzes an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
essential for biosynthesis of complex N-linked oligosaccharides
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
-
-
-
?
UDP-alpha-D-GlcNAc + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[VSG221 protein]
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->3)-alpha-D-Man-(1->6)-alpha-D-Man
?
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->3)-alpha-D-Man-(1->6)-alpha-D-Man
?
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
-
-
-
?
UDP-GlcNAc + alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)-[alpha-D-Man-(1->3)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc
-
-
-
?
additional information
?
-
-
the gene is alternatively spliced and developmmentally regulated
-
-
?
additional information
?
-
-
the enzyme is required for the chain initiation and elongation of the heparan sulfate backbone
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
additional information
additional information
-
Ca2+, Co2+, Cu2+, Hg2+, Mg2+ or Zn2+ cannot replace Mn2+, at 1 or 2.5 mM each
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-deoxy-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
3-O-(4,4-azopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
3-O-(5-aminopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
6-deoxy-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-beta-D-mannosyl-octyl
-
-
O-(4,4-azo)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
O-(5-amino)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
O-(5-iodoacetamido)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
irreversible inhibition
O-pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
O-(4,4-azo)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
irreversible inhibition
O-(4,4-azo)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
alpha-1,6-mannosyl-glycoprotein 2-beta-n-acetylglucosaminyltransferase deficiency
Carbohydrate-deficient glycoprotein syndrome type II. An autosomal recessive N-acetylglucosaminyltransferase II deficiency different from typical hereditary erythroblastic multinuclearity, with a positive acidified-serum lysis test (HEMPAS).
alpha-1,6-mannosyl-glycoprotein 2-beta-n-acetylglucosaminyltransferase deficiency
Hypoglycosylation of a brain glycoprotein (beta-trace protein) in CDG syndromes due to phosphomannomutase deficiency and N-acetylglucosaminyl-transferase II deficiency.
Anemia
Primary defect of congenital dyserythropoietic anemia type II. Failure in glycosylation of erythrocyte lactosaminoglycan proteins caused by lowered N-acetylglucosaminyltransferase II.
Anemia, Dyserythropoietic, Congenital
Defective glycosylation of erythrocyte membrane glycoconjugates in a variant of congenital dyserythropoietic anemia type II: association of low level of membrane-bound form of galactosyltransferase.
Anemia, Dyserythropoietic, Congenital
HEMPAS. Hereditary erythroblastic multinuclearity with positive acidified serum lysis test.
Anemia, Dyserythropoietic, Congenital
Incomplete synthesis of N-glycans in congenital dyserythropoietic anemia type II caused by a defect in the gene encoding alpha-mannosidase II.
Anemia, Dyserythropoietic, Congenital
Primary defect of congenital dyserythropoietic anemia type II. Failure in glycosylation of erythrocyte lactosaminoglycan proteins caused by lowered N-acetylglucosaminyltransferase II.
Congenital Disorders of Glycosylation
Carbohydrate-deficient glycoprotein syndrome type II. An autosomal recessive N-acetylglucosaminyltransferase II deficiency different from typical hereditary erythroblastic multinuclearity, with a positive acidified-serum lysis test (HEMPAS).
Infections
Engineering the baculovirus genome to produce galactosylated antibodies in lepidopteran cells.
Neuroblastoma
Knockdown of N-Acetylglucosaminyltransferase-II Reduces Matrix Metalloproteinase 2 Activity and Suppresses Tumorigenicity in Neuroblastoma Cell Line.
phosphomannomutase deficiency
Hypoglycosylation of a brain glycoprotein (beta-trace protein) in CDG syndromes due to phosphomannomutase deficiency and N-acetylglucosaminyl-transferase II deficiency.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.16
4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37°C
0.55
alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37°C
0.13
alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37°C
0.0166 - 0.19
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
0.062 - 0.557
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
0.0166
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
pH 6.5
0.1
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
37°C
0.19
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
pH 6.5, 37°C
0.062
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293F cells
0.0782
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293S cells
0.115
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant N318A
0.145
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant Y294A
0.17
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant W346A
0.223
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant R198A
0.29
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant E259A
0.557
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant Y344A
0.295
UDP-alpha-D-GlcNAc
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293S cells
0.349
UDP-alpha-D-GlcNAc
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293F cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.000083 - 1.003
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
0.000083
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant E259A
0.0023
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant Y344A
0.005
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant W346A
0.05
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant N318A
0.137
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant R198A
0.167
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant Y294A
0.682
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293S cells
1.003
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293F cells
0.593
UDP-alpha-D-GlcNAc
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293S cells
0.773
UDP-alpha-D-GlcNAc
pH 7.0, 37°C, recombinant wild-type mutant expressed from HEK-293F cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00029 - 16.18
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
0.00029
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant E259A
0.0041
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn
pH 7.0, 37°C, recombinant mutant Y344A