BRENDA - Enzyme Database
show all sequences of 2.4.1.132

The biosynthesis of oligosaccharide-lipids. Partial purification and characterization of mannosyltransferase II

Jensen, J.W.; Schutzbach, J.S.; J. Biol. Chem. 256, 12899-12904 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dolichol
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
dolichyl phosphate
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
Nonidet P-40
activation, up to 0.0225% v/v
Oryctolagus cuniculus
General Stability
General Stability
Organism
glycerol, 0.5% v/v, stabilizes during storage and purification
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
weak
Oryctolagus cuniculus
ATP
weak
Oryctolagus cuniculus
EDTA
-
Oryctolagus cuniculus
GDP
50% inhibition at 0.0006 mM
Oryctolagus cuniculus
GDPglucose
-
Oryctolagus cuniculus
glycerol
1-2% v/v
Oryctolagus cuniculus
GMP
50% inhibition at 0.094 mM
Oryctolagus cuniculus
GTP
50% inhibition at 0.006 mM
Oryctolagus cuniculus
additional information
no inhibition by amphomycin
Oryctolagus cuniculus
NiCl2
-
Oryctolagus cuniculus
UDP
weak
Oryctolagus cuniculus
UDP-N-acetylglucosamine
weak
Oryctolagus cuniculus
UDPglucose
weak
Oryctolagus cuniculus
UTP
weak
Oryctolagus cuniculus
ZnCl2
-
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
tetrasaccharide-diphosphoryl-lipid
detergent-stimulated
Oryctolagus cuniculus
0.00065
-
GDPmannose
detergent-stimulated
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, about 60% as effective as Mg2+
Oryctolagus cuniculus
Co2+
activation, about 4% as effective as Mg2+
Oryctolagus cuniculus
Mg2+
10 mM; requirement
Oryctolagus cuniculus
Mn2+
activation, about 40% as effective as Mg2+
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
no acceptor is dolichol-phosphate
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636752
Oryctolagus cuniculus
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
GDPmannose + tetrasaccharide-diphosphoryl-lipid
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.3
-
Oryctolagus cuniculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dolichol
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
dolichyl phosphate
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
Nonidet P-40
activation, up to 0.0225% v/v
Oryctolagus cuniculus
General Stability (protein specific)
General Stability
Organism
glycerol, 0.5% v/v, stabilizes during storage and purification
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
weak
Oryctolagus cuniculus
ATP
weak
Oryctolagus cuniculus
EDTA
-
Oryctolagus cuniculus
GDP
50% inhibition at 0.0006 mM
Oryctolagus cuniculus
GDPglucose
-
Oryctolagus cuniculus
glycerol
1-2% v/v
Oryctolagus cuniculus
GMP
50% inhibition at 0.094 mM
Oryctolagus cuniculus
GTP
50% inhibition at 0.006 mM
Oryctolagus cuniculus
additional information
no inhibition by amphomycin
Oryctolagus cuniculus
NiCl2
-
Oryctolagus cuniculus
UDP
weak
Oryctolagus cuniculus
UDP-N-acetylglucosamine
weak
Oryctolagus cuniculus
UDPglucose
weak
Oryctolagus cuniculus
UTP
weak
Oryctolagus cuniculus
ZnCl2
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
tetrasaccharide-diphosphoryl-lipid
detergent-stimulated
Oryctolagus cuniculus
0.00065
-
GDPmannose
detergent-stimulated
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, about 60% as effective as Mg2+
Oryctolagus cuniculus
Co2+
activation, about 4% as effective as Mg2+
Oryctolagus cuniculus
Mg2+
10 mM; requirement
Oryctolagus cuniculus
Mn2+
activation, about 40% as effective as Mg2+
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + tetrasaccharide-diphosphoryl-lipid
no acceptor is dolichol-phosphate
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636752
Oryctolagus cuniculus
?
GDPmannose + tetrasaccharide-diphosphoryl-lipid
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
GDPmannose + tetrasaccharide-diphosphoryl-lipid
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.3
-
Oryctolagus cuniculus
Other publictions for EC 2.4.1.132
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736467
Imamura
Human immunodeficiency virus t ...
Mus musculus, Mus musculus C57/BL6J
J. Biol. Chem.
289
9865-9879
2014
-
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1
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1
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1
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3
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7
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7
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1
3
3
1
-
-
735876
Cossins
Congenital myasthenic syndrome ...
Homo sapiens, Mus musculus
Brain
136
944-956
2013
-
-
2
-
3
-
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2
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4
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1
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1
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5
5
-
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-
709008
Kämpf
Biochemical characterization a ...
Saccharomyces cerevisiae
J. Biol. Chem.
284
11900-11912
2009
-
-
-
-
20
-
-
-
1
-
-
1
-
1
-
1
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-
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2
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1
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1
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20
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1
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1
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1
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2
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
707472
O'Reilly
In vitro evidence for the dual ...
Saccharomyces cerevisiae
Biochemistry
45
9593-9603
2006
-
-
1
-
3
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
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1
-
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-
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1
-
-
3
-
-
-
-
-
1
-
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1
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
658848
Gao
Physical interactions between ...
Saccharomyces cerevisiae
Glycobiology
14
559-570
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
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-
-
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1
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-
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-
-
-
636759
Thiel
A new type of congenital disor ...
Homo sapiens, Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Biol. Chem.
278
22498-22505
2003
-
1
2
-
3
-
-
-
-
-
-
3
-
14
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
3
-
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-
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3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
636760
Lellouch
Phytanyl-pyrophosphate-linked ...
Komagataeibacter xylinus
Biochem. Biophys. Res. Commun.
272
290-292
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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2
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1
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2
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-
-
-
-
-
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636761
Doering
-
alpha-1,3-Mannosyltransferase ...
Cryptococcus neoformans
PCT Int. Appl.
2000
38pp
2000
-
1
-
-
-
-
1
-
-
-
-
-
-
1
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1
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1
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1
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636757
Romero
Mnt2p and Mnt3p of Saccharomyc ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Glycobiology
9
1045-1051
1999
-
-
1
-
-
-
-
-
1
-
-
2
-
10
-
-
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-
-
-
-
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6
-
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1
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1
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2
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6
-
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-
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-
-
-
-
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-
636763
Doering
A unique alpha-1,3-mannosyltra ...
Cryptococcus neoformans
J. Bacteriol.
181
5482-5488
1999
-
1
-
-
-
-
3
-
1
-
-
1
-
1
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1
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1
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3
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1
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1
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1
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636764
Reynolds
The high osmolarity glycerol r ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Cell Biol.
143
935-946
1998
-
-
-
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1
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1
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10
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1
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1
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636765
Wiggins
Activity of the yeast MNN1 alp ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Proc. Natl. Acad. Sci. USA
95
7945-7940
1998
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-
-
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1
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10
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2
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1
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2
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-
636766
Shpakov
Yeast dolichol-coupled mannosy ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Zh. Evol. Biokhim. Fiziol.
32
3-18
1996
-
-
1
-
-
-
-
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-
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10
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2
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1
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2
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-
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-
-
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-
636755
Graham
Sorting of yeast alpha 1,3 man ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
Mol. Biol. Cell
6
809-824
1995
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-
1
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1
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1
2
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9
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1
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4
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1
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4
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636756
Graham
Clathrin-dependent localizatio ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae A
J. Cell. Biol.
127
667-678
1994
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1
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1
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1
2
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9
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1
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4
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1
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1
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