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Literature summary for 2.4.1.109 extracted from
- The C-terminal MIR-containing region in the Pmt1 O-mannosyltransferase restrains sporulation and is dispensable for virulence in Beauveria bassiana (2017), Appl. Microbiol. Biotechnol., 101, 1143-1161 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pmt1, recombinant expression of His-tagged full-length wild-type enzyme and of truncated mutant Pmt1DELTA311-902 in Pichia pastoris strain GS115 via transformation by Agrobacterium tumefaciens strain AGL-1, quantitative real-time RT-PCR expression and analysis | Beauveria bassiana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a truncated mutant lacking the C-terminal Pmt1 MIR-containing region (Pmt1DELTA311-902) by targeted gene replacement and construction of the C-terminal Pmt1 MIR-containing region deletion strains and complementation strains. Construction and analysis of the C-terminal Pmt1 MIR-containing region-affected transcriptomes. Complementation by expression of wild-type enzyme. The mutant shows altered conidial yield, conidial germination, hydrophobicity and adhesion, phenotypes, overview | Beauveria bassiana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Beauveria bassiana | 5618 | - |
| membrane | transmembrane helical structure | Beauveria bassiana | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] | Beauveria bassiana | - |
dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein] | - |
? |  |
| dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] | Beauveria bassiana ARSEF 2860 | - |
dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | Beauveria bassiana | - |
dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | Beauveria bassiana ARSEF 2860 | - |
dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beauveria bassiana | J5JB66 | - |
- |
| Beauveria bassiana ARSEF 2860 | J5JB66 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| samples containing both growing mycelia and aerial conidia are harvested from 1:4-diluted SDAY plates grown at 26°C for 15 days, recombinant His-tagged full-length wild-type enzyme and truncated mutant Pmt1DELTA311-902 purified from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, nickel affinity chromatography and gel filtration | Beauveria bassiana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | aerial | Beauveria bassiana | - |
| mycelium | - |
Beauveria bassiana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] | - |
Beauveria bassiana | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] | - |
Beauveria bassiana ARSEF 2860 | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | - |
Beauveria bassiana | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | substrates used are Dol-P-[3H]mannose and 3.5 mM acceptor peptide Ac-YATAV-NH2 | Beauveria bassiana | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | - |
Beauveria bassiana ARSEF 2860 | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
| dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] | substrates used are Dol-P-[3H]mannose and 3.5 mM acceptor peptide Ac-YATAV-NH2 | Beauveria bassiana ARSEF 2860 | dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMT1 | - |
Beauveria bassiana |
| Pmt1 O-mannosyltransferase | - |
Beauveria bassiana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Beauveria bassiana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Beauveria bassiana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the lack of C-terminal Pmt1 MIR-containing region affects transcriptomes, altering the transcription of genes involved in asexual development, e.g. transcription patterns of seven conidiation-associated genes flbA-flbD, fluG, hyd1, and hyd2. Loss of the enzyme's C-terminal Pmt1 MIR-containing region affects mannosylation of the cell wall proteins. The Pmt1DELTA311-902 cells show significant increases in lectin binding when probed with either the ConA or the JAL lectins as compared with the wild-type strain. Increased binding of the WGA lectin is also detected or Pmt1delta311-902 cells, but no obvious difference in reactivity with GNL between the wild-type and Pmt1delta311-902 cells. The mutant shows altered conidial yield, conidial germination, hydrophobicity and adhesion. Phenotypes, overview. Hyphal bodies isolated from insects infected with Pmt1DELTA311-902 cells are elongated and significantly longer than their wild-type counterparts. In addition, hyphal branching is seen in numerous cells, a phenomenon rarely detected in wild-type (and complemented strain) samples | Beauveria bassiana |
| additional information | cell wall composition of wild-type strain and the Pmt1DELTA311-902 mutant strain, overview | Beauveria bassiana |
| physiological function | the C-terminal Pmt1 MIR-containing region affects transcriptomes. Requirement of the C-terminal Pmt1 MIR containing region on Pmt1 O-mannosyltransferase enzyme activity in vivo. The C-terminal MIR-containing domain of Pmt1 is involved in cell wall integrity, cell surface characteristics, and cell adhesion. But the Pmt1 C-terminus domain containing the MIR motifs is dispensable for fungal virulence | Beauveria bassiana |
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