Application | Comment | Organism |
---|---|---|
medicine | using Drosophila as model for investigating the mechanisms of human congenital muscular dystrophies (dystroglycanopathies) resulting from the abnormal glycosylation of alpha-dystroglycan | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants lacking or overexpressing one of the two or both O-mannosyltransferases Rotated Abdomen and Twisted show that both are required for in vivo production of high molecular mass dystroglycan, overexpression of only one isoform does not increase its amount over wild-type, lack of either one of the isoforms or both isoforms lacking double mutant do not produce the high molecular mass dystroglycan | Drosophila melanogaster |
yes | mutants lacking or overexpressing one of the two or both O-mannosyltransferases Rotated Abdomen and Twisted show that both are required for in vivo production of high molecular mass dystroglycan, overexpression of only one isoform does not increase its amount over wild-type, lack of either one of the isoforms or both isoforms lacking double mutant do not produce the high molecular mass dystroglycan | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Drosophila melanogaster | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + alpha-dystroglycan | Drosophila melanogaster | in vitro asssay with both enzyme isoforms (RT and TW), 20 mM Tris, pH 8.0, mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, sugar donor is tritium-labeled, alpha-dystroglycan isoform C contains mucin-type domain that is target of O-mannose modifications in mammals, high grade of O-mannosylation, A isoform without noticeable O-mannosylation, rabbit alpha-dystroglycan as positive control shows lower O-mannosylation | dolichyl phosphate + O-D-mannosyl-[alpha-dystroglycan] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + alpha-dystroglycan | in vitro asssay with both enzyme isoforms (RT and TW), 20 mM Tris, pH 8.0, mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, sugar donor is tritium-labeled, alpha-dystroglycan isoform C contains mucin-type domain that is target of O-mannose modifications in mammals, high grade of O-mannosylation, A isoform without noticeable O-mannosylation, rabbit alpha-dystroglycan as positive control shows lower O-mannosylation | Drosophila melanogaster | dolichyl phosphate + O-D-mannosyl-[alpha-dystroglycan] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Drosophila O-mannosyltransferase Rotated Abdomen | - |
Drosophila melanogaster |
Drosophila O-mannosyltransferase Twisted | - |
Drosophila melanogaster |
O-mannosyltransferase | - |
Drosophila melanogaster |
RT | - |
Drosophila melanogaster |
TW | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | defects in O-mannosylation are associated with muscular dystrophies (dystroglycanopathies) | Drosophila melanogaster |
physiological function | O-mannosylation can modulate the ligand-binding activity of alpha-dystroglycan | Drosophila melanogaster |