Application | Comment | Organism |
---|---|---|
food industry | lactosucrose, a rare trisaccharide formed from sucrose and lactose by enzymatic transglycosylation, is a type of indigestible carbohydrate with a good prebiotic effect. Lactosucrose biosynthesis is efficiently carried out by a purified levansucrase from Leuconostoc mesenteroides B-512 | Leuconostoc mesenteroides |
Cloned (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3) | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | Q5IS34 | - |
- |
Leuconostoc mesenteroides B?512 | Q5IS34 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
791 | - |
purified recombinant His-tagged enzyme, pH 6.5, 50°C, transfructosylation with lactose as acceptor substrate | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + lactose | optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme | Leuconostoc mesenteroides | D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside | i.e. lactosefructose or lactosucrose | ? | |
sucrose + lactose | optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme | Leuconostoc mesenteroides B?512 | D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside | i.e. lactosefructose or lactosucrose | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Leuconostoc mesenteroides |